Nucleic acid from beans extracted by ethanediamine magnetic particles

Abstract

Ethanediamine magnetite nanoparticles (EDAMPs) were used as adsorbents to isolate genomic DNA from various bean-species. A "single-pot" preparation of EDAMPs was described. Further characterization, including transmission electronic microscopy (TEM), scanning electronic microscopy (SEM), thermo-gravimetric analysis (TGA), X-ray powder diffraction (XRD) and Fourier Transform Infrared Spectrophotometer (FT-IR) were used to demonstrate the efficiency of this simple and general identification method. The EDAMPs provided excellent yields of genomic DNA. The isolated DNAwas suitable for use in further applications by the Polymerase Chain Reaction (PCR) for the detection of Genetically Modified (GM) food and non-GM food. The EDAMPs are effective for bioseparation applications.

Electrochemical Detection of Ultra-trace Cu(II) and Interaction Mechanism Analysis Between Amine-Groups Functionalized CoFe 2 O 4 /Reduced Graphene Oxide Composites and Metal Ion

“…Bands around 1,660 and 1670 cm −1 correspond to N−H bending vibration in PEI/CoFe2O4/rGO and EDA/CoFe2O4/rGO composites in Fig. 2(B−c,d), it is -NH2 rather than protonated -NH3 + , lack a characteristic peak located at 2100 cm -1 , indicating the synthesis of amine-groups functionalized CoFe2O4/rGO composites [45][46][47]. The intense band at 3,500 cm −1 indicates the stretching vibration of O−H in H2O molecular.…”