Nucleic acid detection systems for enteroviruses

Rotbart, Harley A.

doi:10.1128/cmr.4.2.156

Cited by 70 publications

(33 citation statements)

References 96 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The clinical features of neurological disease due to non-polio enteroviruses can overlap those caused by herpesviruses. There may be unnecessary hospitalization and anti-herpetic therapy may, therefore, be initiated for patients with mild enterovirus infection (32). However, additional studies are required in order to evaluate the present assay with a greater number of CSF specimens in the clinical context by testing it against well-defined specimens that have been thoroughly characterized by other methods and, finally, in order to assess its clinical significance and its utility in monitoring the efficacy of treatment with antiviral drugs.…”

Section: Discussionmentioning

confidence: 99%

Laboratory Diagnosis of Common Herpesvirus Infections of the Central Nervous System by a Multiplex PCR Assay

et al. 2001

View full text Add to dashboard Cite

A sensitive multiplex PCR assay for single-tube amplification that detects simultaneous herpes simplex virus type 1 (HSV-1), herpes simplex virus type 2 (HSV-2), varicella-zoster virus (VZV), human cytomegalovirus (CMV), and Epstein-Barr virus (EBV) is reported with particular emphasis on how the method was optimized and carried out and its sensitivity was compared to previously described assays. The assay has been used on a limited number of clinical samples and must be thoroughly evaluated in the clinical context. A total of 86 cerebrospinal fluid (CSF) specimens from patients which had the clinical symptoms of encephalitis, meningitis or meningoencephalitis were included in this study. The sensitivity of the multiplex PCR was determined to be 0.01 and 0.03 50% tissue culture infective doses/the reciprocal of the highest dilution positive by PCR for HSV-1 and HSV-2 respectively, whereas for VZV, CMV and EBV, 14, 18, and 160 ag of genomic DNA were detected corresponding to 48, 66, and 840 genome copies respectively. Overall, 9 (10.3%) of the CSF samples tested were positive in the multiplex PCR. HSV-1 was detected in three patients (3.5%) with encephalitis, VZV was detected in four patients (4.6%) with meningitis, HSV-2 was detected in one neonate (1.16%), and CMV was also detected in one neonate (1.16%). None of the samples tested was positive for the EBV genome. None of the nine positive CSF samples presented herpesvirus coinfection in the central nervous system. Failure of DNA extraction or failure to remove any inhibitors of DNA amplification from CSF samples was avoided by the inclusion in the present multiplex PCR assay of ␣-tubulin primers. The present multiplex PCR assay detects simultaneously five different herpesviruses and sample suitability for PCR in a single amplification round of 40 cycles with an excellent sensitivity and can, therefore, provide an early, rapid, reliable noninvasive diagnostic tool allowing the application of antiviral therapy on the basis of a specific viral diagnosis. The results of this preliminary study should prompt a more exhaustive analysis of the clinical value of the present multiplex PCR assay.

show abstract

Section: Discussionmentioning

confidence: 99%

Laboratory Diagnosis of Common Herpesvirus Infections of the Central Nervous System by a Multiplex PCR Assay

et al. 2001

View full text Add to dashboard Cite

show abstract

“…In the present study, the set of primers used for the human EVs were capable of amplifying the 650-bp fragment encoding the 3) one third of 5) NTR, the entire VP4, and a 5) one third of VP2 of all prototype strains of human EVs. These primers have also been shown to be capable of amplifying the HRV genome to produce a fragment that is 120 bp shorter than that of EVs [4,6,7]. This led us to use the primers to develop an RT-PCR and phylogenetic analysis-based diagnosis of HRV.…”

Section: Discussionmentioning

confidence: 99%

Human Rhinovirus 87 Identified as Human Enterovirus 68 by VP4-Based Molecular Diagnosis

Ishiko

Miura²,

Shimada³

et al. 2002

Intervirology

View full text Add to dashboard Cite

Human rhinoviruses (HRVs) are the major cause of respiratory infections. We developed a diagnostic method for HRVs based on the reverse-transcription polymerase chain reaction (RT-PCR) and VP4-based phylogenetic analysis. A set of primers used in the RT-PCR of human enteroviruses (EVs) appeared to be capable of amplifying all prototype strains of HRVs, each of which generated a 530-bp fragment. The single exception was HRV-87, which generated a 650-bp fragment, as observed in human EVs. The VP4 nucleotide sequence of HRV-87 showed more than 97% nucleotide identity with human EV-68, and formed a monophyletic cluster along with the prototype strain of EV-68 in the human EV-D cluster. HRV-87 showed the second highest homology (76.8%) with EV-70, another member of the human EV-D, in a sample of 66 human EVs and 12 HRVs. Therefore, HRV-87 should be reclassified into the cluster containing human EV-68.

show abstract

“…The recent advances toward the adaptation of nucleic acid-based detection systems to clinical EV diseases 17,18 and the promising anti-EV compounds developing 19,20 will aid the clinician in rapid diagnosis and the use of specific antiviral therapy. Efforts should be made in the future to do larger prospective studies in this region of the world for a better understanding of the epidemiology of EV infection among both symptomatic and asymptomatic patients.…”

Section: Discussionmentioning

confidence: 99%

“…; 17(1): [16][17][18][19] Enterovirus (EV) infections are responsible for significant and frequent human illness throughout the world. Control of polioviruses in much of the world, including Saudi Arabia, has focused attention on the nonpolio EV.…”

Section: S Al-hajjar J Akhter H Arishi Smh Qadri Enteroviruses Atmentioning

confidence: 99%

Enteroviruses at a Tertiary Care Center in Saudi Arabia, 1989-1995

et al. 1997

View full text Add to dashboard Cite

Enteroviruses (EVs) are among the most common viral pathogens affecting humans. Enterovirus (EV) infections occur worldwide in temperate climates with a marked summer/fall season and have a high year-round incidence in tropical and subtropical areas. In Saudi Arabia, the epidemiology of enteroviruses is unknown. Of the clinical specimens submitted for diagnosis during a six-year period (1989)(1990)(1991)(1992)(1993)(1994)(1995) at King Faisal Specialist Hospital and Research Centre (KFSH&RC) in Riyadh, a wide variety of diseases were associated with the isolation of enteroviruses. Among the diseases were herpangina, sepsis-like illness, hand-foot-and-mouth syndrome, laryngotracheitis (croup), aseptic meningitis, pneumonia, and gastrointestinal illness. During the six-year study, a variety of enteroviruses were isolated. Although epidemics were seen in mid-winter and early spring, EV which may cause sporadic diseases were isolated in each of the 12 months. Health care providers must become aware of the epidemic potential not only in mid-winter and early spring, but also throughout the year, as this will aid in the diagnosis and management of this illness.

show abstract

Nucleic acid detection systems for enteroviruses

Cited by 70 publications

References 96 publications

Laboratory Diagnosis of Common Herpesvirus Infections of the Central Nervous System by a Multiplex PCR Assay

Laboratory Diagnosis of Common Herpesvirus Infections of the Central Nervous System by a Multiplex PCR Assay

Human Rhinovirus 87 Identified as Human Enterovirus 68 by VP4-Based Molecular Diagnosis

Enteroviruses at a Tertiary Care Center in Saudi Arabia, 1989-1995

Contact Info

Product

Resources

About