Nucleic acid detection based on the use of microbeads: a review

Rödiger, Stefan; Liebsch, Claudia; Schmidt, Carsten; Lehmann, W.; Resch‐Genger, Ute; Schedler, Uwe; Schierack, Peter

doi:10.1007/s00604-014-1243-4

Cited by 77 publications

(68 citation statements)

References 196 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is also possible that non-specific products, including primer dimers and other long DNAs, could be amplified by DNA amplification methods similar to PCR. This problem could potentially be minimized by using a sequence-specific attachment scheme, such as previously described hybridization-based DNA-microbead attachment methods [ 23 ]. We will examine this in future studies.…”

Section: Resultsmentioning

confidence: 99%

Comparison of Sensitivity and Quantitation between Microbead Dielectrophoresis-Based DNA Detection and Real-Time PCR

2017

View full text Add to dashboard Cite

In this study, we describe a microbead-based method using dielectrophoresis (DEP) for the fast detection of DNA amplified by polymerase chain reaction (PCR). This electrical method measures the change in impedance caused by DEP-trapped microbeads to which biotinylated target DNA molecules are chemically attached. Using this method, measurements can be obtained within 20 min. Currently, real-time PCR is among the most sensitive methods available for the detection of target DNA, and is often used in the diagnosis of infectious diseases. We therefore compared the quantitation and sensitivity achieved by our method to those achieved with real-time PCR. We found that the microbead DEP-based method exhibited the same detection limit as real-time PCR, although its quantitative detection range was slightly narrower at 10–105 copies/reaction compared with 10–107 copies/reaction for real-time PCR. Whereas real-time PCR requires expensive and complex instruments, as well as expertise in primer design and experimental principles, our novel method is simple to use, inexpensive, and rapid. This method could potentially detect viral and other DNAs efficiently in combination with conventional PCR.

show abstract

Section: Resultsmentioning

confidence: 99%

Comparison of Sensitivity and Quantitation between Microbead Dielectrophoresis-Based DNA Detection and Real-Time PCR

2017

View full text Add to dashboard Cite

show abstract

“…Diagnostic applications include microRNA profiling, gene expression analysis, pathogen quantification, and cancer diagnostics (2,3 ). There is an ongoing development of novel devices on the basis of capillary systems, microfluidics, point-of-care testing, microbeads, and related technologies (6,(7)(8)(9)(10)(11)(12).…”

confidence: 99%

Impact of Smoothing on Parameter Estimation in Quantitative DNA Amplification Experiments

et al. 2015

Self Cite

View full text Add to dashboard Cite

BACKGROUND: Quantification cycle (Cq) and amplification efficiency (AE) are parameters mathematically extracted from raw data to characterize quantitative PCR (qPCR) reactions and quantify the copy number in a sample. Little attention has been paid to the effects of preprocessing and the use of smoothing or filtering approaches to compensate for noisy data. Existing algorithms largely are taken for granted, and it is unclear which of the various methods is most informative. We investigated the effect of smoothing and filtering algorithms on amplification curve data.

show abstract

“…Monodisperse microbeads and microdroplets are indispensable for a number of applications ranging from research to biomedical and industrial applications. 1 Common use of these objects are as spacers for displays and chips, size standards, bioassays, 2,3 coupled resonator optical waveguides 4 and drug delivery systems in pharmaceutical and biomedical fields. [5][6][7] Monodisperse microbeads from materials such as polystyrene and polyĲmethyl methacrylate) are mostly being manufactured by chemical methods, for example dispersion polymerization.…”

Section: Introductionmentioning

confidence: 99%

Optical-resonance-assisted generation of super monodisperse microdroplets and microbeads with nanometer precision

2020

View full text Add to dashboard Cite

show abstract

Nucleic acid detection based on the use of microbeads: a review

Cited by 77 publications

References 196 publications

Comparison of Sensitivity and Quantitation between Microbead Dielectrophoresis-Based DNA Detection and Real-Time PCR

Comparison of Sensitivity and Quantitation between Microbead Dielectrophoresis-Based DNA Detection and Real-Time PCR

Impact of Smoothing on Parameter Estimation in Quantitative DNA Amplification Experiments

Optical-resonance-assisted generation of super monodisperse microdroplets and microbeads with nanometer precision

Contact Info

Product

Resources

About