Nucleic acid delivery: Where material sciences and bio-sciences meet

Remaut, Katrien; Sanders, Niek N.; Geest, Bruno G. De; Braeckmans, Kevin; Demeester, Jo; Smedt, Stefaan C. De

doi:10.1016/j.mser.2007.06.001

Cited by 90 publications

(104 citation statements)

References 367 publications

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“…The ideal delivery system should guide the nucleic acids to their target cells, increase their cellular uptake and help the nucleic acids to escape from the endosomal compartment into the cytoplasm of the cells. Also, the possible degradation of the nucleic acids in the intracellular environment should be taken into account, as the intact sequence is necessary to maintain their biological activity (1).…”

Section: Introductionmentioning

confidence: 99%

Lysosomal capturing of cytoplasmic injected nanoparticles by autophagy: An additional barrier to non viral gene delivery

Remaut

Oorschot²,

Braeckmans

et al. 2014

Journal of Controlled Release

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Autophagy or 'self-eating' is a process by which defective organelles and foreign material can be cleared from the cell's cytoplasm and delivered to the lysosomes in which degradation occurs. It remains an open question, however, whether nanoparticles that did not enter the cell through endocytosis can also be captured from the cytoplasm by autophagy. We demonstrate that nanoparticles that are introduced directly in the cytoplasm of the cells by microinjection, can trigger an autophagy response. Moreover, both polystyrene beads and plasmid DNA containing poly-ethylene-imine complexes colocalize with autophagosomes and lysosomes, as was confirmed by electron microscopy.This indicates that cytoplasmic capturing of nanoparticles can occur by an autophagy response. The capturing of nanoparticles from the cytoplasm most likely limits the time frame in which efficient nucleic acid delivery can be obtained. Hence, autophagy forms an additional barrier to non-viral gene delivery, a notion that was not often taken into account before. Furthermore, these findings urges us to reconsider the idea that a single endosomal escape event is sufficient to have the long-lasting presence of nanoparticles in the cytoplasm of the cells.

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Section: Introductionmentioning

confidence: 99%

Lysosomal capturing of cytoplasmic injected nanoparticles by autophagy: An additional barrier to non viral gene delivery

Remaut

Oorschot²,

Braeckmans

et al. 2014

Journal of Controlled Release

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“…This low expression efficiency can be explained by taking into account several hurdles which are intrinsically associated with lipo-or polyplex-based gene delivery: 1) cellular uptake of the complexes, 2) the translocation of the complexes from the endosomes to the cytoplasm, 3) dissociation of the DNA from the lipid or polymer carrier and 4) translocation of the DNA from the cytoplasm to the nuclear compartment [11,97,98]. Which one of these barriers limits the transfection efficiency in the most pronounced way is debatable.…”

Section: Mitotic Partitioning Of Inorganic Quantum Dots Gold and Iromentioning

confidence: 99%

Intracellular partitioning of cell organelles and extraneous nanoparticles during mitosis

Symens

Soenen

Rejman

et al. 2012

Advanced Drug Delivery Reviews

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“…Real-time multiple particle tracking (MPT) has been used by several investigators to study the endocytosis of nanomedicines and reveal new insights into their complex transport (Bausinger et al 2006;Payne 2007;Suh et al 2003;Suk et al 2007). Other fluorescence microscopy methods, including Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP), and fluorescence correlation spectroscopy (FCS), are widely used to study intracellular trafficking of nanomedicines within living cells (Remaut et al 2007). While FRAP and FCS measure averaged transport properties of a large ensemble of nanoparticles, MPT provides detailed dynamic transport information of each individual nanoparticle.…”

Section: Biophysical Methods To Study Traffickingmentioning

confidence: 99%

The emergence of multiple particle tracking in intracellular trafficking of nanomedicines

Kim

2012

Biophys Rev

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A growing number of nanoparticle systems, termed "nanomedicines", are being developed for diagnostic and therapeutic applications. Nanoparticles can employ various cellular entry pathways and trafficking mechanisms to effectively deliver drugs, biomolecules, and imaging agents to precise sub-cellular locations. However, the dynamic transport of nanoparticles through the complex intracellular environment is not well understood, having been primarily studied with static or bulk averaged methods in the past. Such techniques do not provide detailed information regarding the transport mechanism and rates of individual nanoparticles, where understanding of the interaction of nanoparticles with the cellular environment remains incomplete. Recent advances in live-cell fluorescence microscopy and real-time multiple particle tracking (MPT) have facilitated an improved understanding of cell trafficking pathways. Understanding the dynamic transport of nanoparticles as they are delivered into complex cellular components may lead to rational improvements in the design of nanomedicines. This review discusses different cellular uptake and trafficking pathways of nanomedicines, briefly highlights current fluorescence microscopy tools, and provides examples from the recent literature on the use of MPT and its applications.

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Nucleic acid delivery: Where material sciences and bio-sciences meet

Cited by 90 publications

References 367 publications

Lysosomal capturing of cytoplasmic injected nanoparticles by autophagy: An additional barrier to non viral gene delivery

Lysosomal capturing of cytoplasmic injected nanoparticles by autophagy: An additional barrier to non viral gene delivery

Intracellular partitioning of cell organelles and extraneous nanoparticles during mitosis

The emergence of multiple particle tracking in intracellular trafficking of nanomedicines

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