Nucleic Acid Carriers Based on Precise Polymer Conjugates

Troiber, Christina; Wagner, Ernst

doi:10.1021/bc200251r

Cited by 70 publications

(50 citation statements)

References 149 publications

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“…7,11,15,16,22,36 Receptor-mediated endocytosis can enhance the cellular uptake of nanoparticles. 11,16,22,31 In our experiments, the U-87 cells could not be transfected effectively with unmodified PEI nanoparticles at an N/P ratio of 10 ( Figures 3 and 4). In general, the VTW peptide facilitated the transfection of PEI nanoparticles into U-87 cells via receptor-mediated uptake, while it reduced the cytotoxicity (Figure 4) due to less residual positive charge on the surface of the nanoparticles.…”

Section: Discussionmentioning

confidence: 69%

“…Many techniques, including purification, preparation, and chemical modification, have been used to improve the properties of nanoparticles. 16,31 A heterobifunctional crosslinker, SPDP, has an amine-reactive radical called the N-hydroxysuccinimide ester and a sulfhydryl-reactive radical called 2-pyridyldithio. [32][33][34] This crosslinker guaranteed that PEI-PDT and VTW were conjugated accurately.…”

Section: Discussionmentioning

confidence: 99%

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A peptide-mediated targeting gene delivery system for malignant glioma cells

Wang¹,

Ning

Wang³

et al. 2013

IJN

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Glioblastoma multiforme (GBM) is the most common and malignant glioma. Although there has been considerable progress in treatment strategies, the prognosis of many patients with GBM remains poor. In this work, polyethylenimine (PEI) and the VTWTPQAWFQWV (VTW) peptide were modified and synthesized into GBM-targeting nanoparticles. The transfection efficiency of U-87 (human glioblastoma) cells was evaluated using fluorescence microscopy and flow cytometry. Cell internalization was investigated to verify the nanoparticle delivery into the cytoplasm. Results showed that the methods of polymer conjugation and the amount of VTW peptide were important factors to polymer synthesis and transfection. The PEI-VTW 20 nanoparticles increased the transfection efficiency significantly. This report describes the use of VTW peptide-based PEI nanoparticles for intracellular gene delivery in a GBM cell-specific manner.

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Section: Discussionmentioning

confidence: 69%

Section: Discussionmentioning

confidence: 99%

A peptide-mediated targeting gene delivery system for malignant glioma cells

Wang¹,

Ning

Wang³

et al. 2013

IJN

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“…Currently, the use of polymer for siRNA or drug delivery have been reported extensively (Nimesh et al, 2011;Singha et al, 2011;Troiber and Wagner, 2011;Duncan, 2011). RNA is a polymer (polynucleic acid).…”

Section: Defined Size Structure and Stoichiometrymentioning

confidence: 99%

Uniqueness, Advantages, Challenges, Solutions, and Perspectives in Therapeutics Applying RNA Nanotechnology

Guo¹,

Haque²,

Hallahan³

et al. 2013

RNA Nanotechnology and Therapeutics

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The field of RNA nanotechnology is rapidly emerging. RNA can be manipulated with the simplicity characteristic of DNA to produce nanoparticles with a diversity of quaternary structures by self-assembly. Additionally RNA is tremendously versatile in its function and some RNA molecules display catalytic activities much like proteins. Thus, RNA has the advantage of both worlds. However, the instability of RNA has made many scientists flinch away from RNA nanotechnology. Other concerns that have deterred the progress of RNA therapeutics include the induction of interferons, stimulation of cytokines, and activation of other immune systems, as well as short pharmacokinetic profiles in vivo. This review will provide some solutions and perspectives on the chemical and thermodynamic stability, in vivo half-life and biodistribution, yield and production cost, in vivo toxicity and side effect, specific delivery and targeting, as well as endosomal trapping and escape.

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“…Various strategies for RNA delivery have been explored, such as the application of lipid-based delivery systems (lipoplexes and liposomes) [4][5][6] , nanoparticles [7][8][9] , viruses 10 , peptide-based systems 7 , polymers [11][12][13][14] and gold-based materials [15][16][17][18] .…”

Section: Introductionmentioning

confidence: 99%

Phosphonium Polymethacrylates for Short Interfering RNA Delivery: Effect of Polymer and RNA Structural Parameters on Polyplex Assembly and Gene Knockdown

et al. 2015

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(2015) Phosphonium polymethacrylates for siRNA delivery: effect of polymer and RNA structural parameters on polyplex assembly and gene knockdown. Biomacromolecules, 16 (11). pp. 3480-3490. ISSN 1525-7797 Access from the University of Nottingham repository: http://eprints.nottingham.ac.uk/35894/1/Phosphonium%20Polymethacrylates%20for %20Short%20Interfering%20RNA%20Delivery%20-%20%20Effect%20of%20Polymer %20and%20RNA%20Structural%20Parameters%20on%20Polyplex%20Assembly%20and %20Gene%20Knockdown%20-%20AAM.pdf Copyright and reuse:The Nottingham ePrints service makes this work by researchers of the University of Nottingham available open access under the following conditions. This article is made available under the Creative Commons Attribution Non-commercial licence and may be reused according to the conditions of the licence. For more details see: http://creativecommons.org/licenses/by-nc/2.5/ A note on versions:The version presented here may differ from the published version or from the version of record. If you wish to cite this item you are advised to consult the publisher's version. Please see the repository url above for details on accessing the published version and note that access may require a subscription. ABSTRACT: Synthetic polymers containing quaternary phosphonium salts are an emerging class of materials for the delivery of oligo/polynucleotides. In this work, cationic phosphonium saltcontaining polymethacrylates -and their corresponding ammonium analogues-were synthesized by RAFT polymerization. Both the nature of the charged heteroatom (N vs. P) and the length of the spacer separating the cationic units along the polymer backbone (oxyethylene vs. trioxyethylene) were systematically varied. Polymers efficiently bound siRNA at N + /P -or P + /Pratios of 2 and above. At a 20:1 ratio, small polyplexes (Rh: 4-15 nm) suitable for cellular uptake were formed that displayed low cytotoxicity. Whilst siRNA polyplexes from both ammonium and phosphonium polymers were efficiently internalised by GFP-expressing 3T3 cells, no knockdown of GFP expression was observed. However, 65% Survivin gene knockdown was observed when short interfering RNA (siRNA) was replaced with novel, multimerised long interfering liRNA 2 (liRNA) in HeLa cells, demonstrating the importance of RNA macromolecular architecture on RNA-mediated gene silencing.

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Nucleic Acid Carriers Based on Precise Polymer Conjugates

Cited by 70 publications

References 149 publications

A peptide-mediated targeting gene delivery system for malignant glioma cells

A peptide-mediated targeting gene delivery system for malignant glioma cells

Uniqueness, Advantages, Challenges, Solutions, and Perspectives in Therapeutics Applying RNA Nanotechnology

Phosphonium Polymethacrylates for Short Interfering RNA Delivery: Effect of Polymer and RNA Structural Parameters on Polyplex Assembly and Gene Knockdown

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