Nucleic acid carrier composed of a branched fatty acid lysine conjugate—Interaction studies with blood components

Giselbrecht, Julia; Wiedemann, Sophia J.; Pinnapireddy, Shashank Reddy; Goergen, Nathalie; Loppnow, Harald; Sedding, Daniel; Erdmann, Frank; Hause, Gerd; Lúcio, Marlene; Langner, Andreas; Wölk, Christian

doi:10.1016/j.colsurfb.2019.110547

Cited by 7 publications

(6 citation statements)

References 40 publications

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“…The protein abundance of corona is affected by particle size and charge, which vary with lipid compositions. 41 , 55 , 56 Ren et al. 57 investigated whether liposomes with a slight negative charge might enhance their blood circulation time due to reduced combinations with plasma proteins and blood cells compared to CLs.…”

Section: Main Textmentioning

confidence: 99%

“…The protein abundance of corona is affected by particle size and charge, which vary with lipid compositions. 41,55,56 Ren et al 57 investigated whether liposomes with a slight negative charge might enhance their blood circulation time due to reduced combinations with plasma proteins and blood cells compared to CLs. However, after extensive experiments on the effects of lipid compositions on corona protein components, it has been discovered that not all of the formation of PC has an adverse effect on CLs.…”

Section: The Extracellular Barriersmentioning

confidence: 99%

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Barriers and Strategies of Cationic Liposomes for Cancer Gene Therapy

Liu

Zhang

Zhu

et al. 2020

Molecular Therapy - Methods & Clinical Development

133

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Cationic liposomes (CLs) have been regarded as the most promising gene delivery vectors for decades with the advantages of excellent biodegradability, biocompatibility, and high nucleic acid encapsulation efficiency. However, the clinical use of CLs in cancer gene therapy is limited because of many uncertain factors in vivo . Extracellular barriers such as opsonization, rapid clearance by the reticuloendothelial system and poor tumor penetration, and intracellular barriers, including endosomal/lysosomal entrapped network and restricted diffusion to the nucleus, make CLs not the ideal vector for transferring extrinsic genes in the body. However, the obstacles in achieving productive therapeutic effects of nucleic acids can be addressed by tailoring the properties of CLs, which are influenced by lipid compositions and surface modification. This review focuses on the physiological barriers of CLs against cancer gene therapy and the effects of lipid compositions on governing transfection efficiency, and it briefly discusses the impacts of particle size, membrane charge density, and surface modification on the fate of CLs in vivo , which may provide guidance for their preclinical studies.

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Section: Main Textmentioning

confidence: 99%

Section: The Extracellular Barriersmentioning

confidence: 99%

Barriers and Strategies of Cationic Liposomes for Cancer Gene Therapy

Liu

Zhang

Zhu

et al. 2020

Molecular Therapy - Methods & Clinical Development

133

View full text Add to dashboard Cite

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“…One possible reason may be that the lipid complex is positively charged in the modifier, because the increased positive charge will increase the possibility of activation of the complement system. Another reason may be the structural change of the coronary protein serum protein, which was confirmed by Giselbrecht's research [42] . Studies on PEGylated liposomes have found that, although the mechanism is not clear, the activation of complement caused by PEGylated liposomes has been attributed to the anionic phosphate portion of the PEG-lipid conjugate.…”

Section: Hemocompatibility Characteristics or Improvement Strategies Of Various Nanocarriersmentioning

confidence: 75%

“…The blood compatibility of liposomes is also one of the factors that are utilized, and it also has characteristics that are different from other nano drug delivery systems ( Fig 2 ). Giselbrecht screened the branched-chain fatty acid lysine conjugate T14diLys (a newly designed cationic lipid for lipid transfection) and prepared a lipid preparation, which achieved good blood compatibility [42] . Kuznetsova used 2% sialic acid Lewis X/A tetrasaccharide based on 8:1 (mol) natural phosphatidylcholine (PC) and phosphatidylinositol (PI) ligand modification, and proved that liposomes can be considered non-hemolytic, and at the same time will reduce the plasma clotting ability [43] .…”

Section: Hemocompatibility Characteristics or Improvement Strategies Of Various Nanocarriersmentioning

confidence: 99%

Relationship and improvement strategies between drug nanocarrier characteristics and hemocompatibility: What can we learn from the literature

Guo

Shi

et al. 2021

Asian Journal of Pharmaceutical Sciences

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This article discusses the various blood interactions that may occur with various types of nano drug-loading systems. Nanoparticles enter the blood circulation as foreign objects. On the one hand, they may cause a series of inflammatory reactions and immune reactions, resulting in the rapid elimination of immune cells and the reticuloendothelial system, affecting their durability in the blood circulation. On the other hand, the premise of the drug-carrying system to play a therapeutic role depends on whether they cause coagulation and platelet activation, the absence of hemolysis and the elimination of immune cells. For different forms of nano drug-carrying systems, we can find the characteristics, elements and coping strategies of adverse blood reactions that we can find in previous researches. These adverse reactions may include destruction of blood cells, abnormal coagulation system, abnormal effects of plasma proteins, abnormal blood cell behavior, adverse immune and inflammatory reactions, and excessive vascular stimulation. In order to provide help for future research and formulation work on the blood compatibility of nano drug carriers.

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“…This was consistent with results for liposomes composed of a branched fatty acid lysine on serum proteins. [36] hFIB is composed of three pairs of nonidentical chains (Aα2, Bβ2, and γ2) and is negatively charged at physiological pH values. [28] In this study, the K SV value was greater for the hFIB-SG-CCDs compared with for the other three proteins of the SG-CCD systems, and the value of E a for the hFIB-SG-CCDs was smaller than those of the HSA-SG-CCD and hTRF-SG-CCD systems.…”

Section: Effect Of Sg-ccds On In Vitro Blood Coagulationmentioning

confidence: 99%

Synthesis of cationic carbon dots and their effects on human serum proteins and in vitro blood coagulation

et al. 2021

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Cationic carbon dots (CCDs) are a promising alternative to gene-delivery systems, and good biosafety levels are crucial for their in vivo use. In this study, spherical and monodispersed CCDs with an average surface potential of +28.7 mV were prepared using sucrose and glutamate (denoted SG-CCDs) using a one-pot autoclave-assisted method. Molecular interactions between the SG-CCDs and four major human serum proteins (albumin, immunoglobulin G, fibrinogen, and transferrin) were investigated.The results were further verified on human serum, and the effect of the SG-CCDs on in vitro blood coagulation was examined. The results showed that the fluorescence of human serum was clearly quenched by the SG-CCDs through a dynamic collision mechanism. Moreover, SG-CCDs at a concentration of 20 μM exhibited minor effects on the secondary structure of human serum. The activated partial thromboplastin and prothrombin time as well as the fibrinogen concentration were not changed, indicating that the SG-CCDs did not interfere with the coagulation process. This study provided an understandable background on the behaviour of CCDs in clinical applications.

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Nucleic acid carrier composed of a branched fatty acid lysine conjugate—Interaction studies with blood components

Cited by 7 publications

References 40 publications

Barriers and Strategies of Cationic Liposomes for Cancer Gene Therapy

Barriers and Strategies of Cationic Liposomes for Cancer Gene Therapy

Relationship and improvement strategies between drug nanocarrier characteristics and hemocompatibility: What can we learn from the literature

Synthesis of cationic carbon dots and their effects on human serum proteins and in vitro blood coagulation

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