Nucleic Acid Association to Human Prostasomes

Olsson, Inge; Ronquist, Gunnar

doi:10.3109/01485019008986852

Cited by 24 publications

(15 citation statements)

References 21 publications

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“…The presence of prostasomes in fractions was demonstrated by assay for amino-peptidase activity, 16 and spectrophotometry at 280 nm. 17 Prostasome containing fractions were pooled, and the protein concentration measured. 18 The suspension was subdivided into 1 ml aliquots and frozen at À201C.…”

Section: Prostasomesmentioning

confidence: 99%

Seminal prostasomes inhibit the angiogenesis activity of rat aortic rings

Delves

Goyal

Lwaleed³

et al. 2006

Prostate Cancer Prostatic Dis

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Prostasomes are organelles secreted by prostatic epithelial cells, and are believed to have a role in fertility and prostatic disease. They are known to influence sperm motility and the acrosome reaction, and are thought to have a role in cell transformation, immunosuppression, proliferation, facilitation of local invasion, and angiogenesis. Previously, we have demonstrated the inhibitory effect of prostasomes derived from human semen on angiogenesis using HUVEC cells grown on matrigel. In this study, we use the rat aortic ring assay system, arguably a closer reflection of the in vivo situation. Quantification was by a spectrophotometric method, and underlying mechanisms assessed. Prostasomes demonstrated a clear inhibition of angiogenesis, and this effect persisted after heat treatment of prostasomes to denature protein. This fits with other known effects of prostasomes known to be due to the membrane lipid component, which is unusually high in sphingomyelin and cholesterol.

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Section: Prostasomesmentioning

confidence: 99%

Seminal prostasomes inhibit the angiogenesis activity of rat aortic rings

Delves

Goyal

Lwaleed³

et al. 2006

Prostate Cancer Prostatic Dis

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show abstract

“…The presence of prostasomes in fractions was demonstrated by assay for amino-peptidase activity, 15 and spectrophotometry at 280 nm. 16 Prostasome containing fractions were pooled, and the protein concentration measured. 17 The suspension was subdivided into 1 ml aliquots and frozen at À201C.…”

Section: Preparation Of Prostasomesmentioning

confidence: 99%

In vitro inhibition of angiogenesis by prostasomes

Delves

Stewart²,

Lwaleed

et al. 2005

Prostate Cancer Prostatic Dis

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Prostasomes are biologically active organelles that are secreted by human prostate epithelial cells, and it is believed that they have a role in prostatic disease. We studied the effect of prostasomes on the human umbilical vein endothelial cell (HUVEC)/Matrigel model of angiogenesis, and the association of labelled prostasomes with HUVECs. The growth inhibitory effect of prostasomes on HUVECs was assayed by spectrophotometric measurement of residual biomass. Preparations of HUVECs on a Matrigel base were exposed to prostasomes, and the development of capillary-like networks was quantified. Prostasomes were labelled with PKH-26, and cultured with HUVECs. Prostasomes were not shown to have a significant effect on HUVEC survival. Angiogenesis assays showed inhibition. The PKH-26-labelled particles were shown to have adhered to the HUVECs. This study adds the inhibition of an in vitro correlate of angiogenesis to the known actions of prostasomes.

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“…Furthermore, exosomes are usually characterized by a bilayer of membranes, whereas prostasomes can have a multilayer membrane [74]. Moreover, in prostasomes, chromosomal DNA, which appears to be absent in exosomes, has been reported [71, 75]. In addition to lipids and DNA, the cargo of prostasomes could also consist of proteins, such as enzymes, transport proteins, structural proteins, signal transduction proteins, and GTP-binding proteins [76].…”

Section: Extracellular Vesiclesmentioning

confidence: 99%

Extracellular Vesicles in Prostate Cancer: New Future Clinical Strategies?

Giusti

Dolo

2014

BioMed Research International

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Prostate cancer (PCa) is the most common cancer—excluding skin tumors—in men older than 50 years of age. Over time, the ability to diagnose PCa has improved considerably, mainly due to the introduction of prostate-specific antigen (PSA) in the clinical routine. However, it is important to take into account that although PSA is a highly organ-specific marker, it is not cancer-specific. This shortcoming suggests the need to find new and more specific molecular markers. Several emerging PCa biomarkers have been evaluated or are being assessed for their potential use. There is increasing interest in the prospective use of extracellular vesicles as specific markers; it is well known that the content of vesicles is dependent on their cellular origin and is strongly related to the stimulus that triggers the release of the vesicles. Consequently, the identification of a disease-specific molecule (protein, lipid or RNA) associated with vesicles could facilitate their use as novel biological markers. The present review describes several in vitro studies that demonstrate the role of vesicles in PCa progression and several in vivo studies that highlight the potential use of vesicles as PCa biomarkers.

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Nucleic Acid Association to Human Prostasomes

Cited by 24 publications

References 21 publications

Seminal prostasomes inhibit the angiogenesis activity of rat aortic rings

Seminal prostasomes inhibit the angiogenesis activity of rat aortic rings

In vitro inhibition of angiogenesis by prostasomes

Extracellular Vesicles in Prostate Cancer: New Future Clinical Strategies?

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