Nucleic acid aptamers in diagnosis of colorectal cancer

Ahmadyousefi, Yaghoub; Malih, Sara; Mirzaee, Younes; Saidijam, Massoud

doi:10.1016/j.biochi.2018.09.009

Cited by 25 publications

(11 citation statements)

References 85 publications

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“…Aptamers can be used in the diagnosis, prognosis, and therapy (theranosis) of cancer. Recently, a new technique of cell-based SELEX has been developed and used in CRC; this technique exhibits higher affinity and specificity than SELEX since the target cells are used to screen aptamers ( Cui et al, 2016 ; Chen et al, 2017 ; Ahmadyousefi et al, 2019 ).…”

Section: Different Formulations Of Lactoferrin With Potential Activit...mentioning

confidence: 99%

Lactoferrin: A Nutraceutical with Activity against Colorectal Cancer

et al. 2022

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Homeostasis in the human body results from the tight regulation of several events, since too little inflammation disrupts the process of tissue repair and remodeling, whereas too much exerts a collateral effect by causing tissue damage with life-threatening consequences. In some clinical conditions, such as inflammatory bowel disease (IBD), inflammation functions as a double-edged sword by either enabling or inhibiting cancer development and progression. Generally, cancer develops through evasion mechanisms that regulate cell growth, causing a high rate of uncontrolled proliferation, and mechanisms for evading cell death, such as apoptosis. Moreover, chronic inflammation is a factor that contributes to colorectal cancer (CRC), as observed in individuals with IBD; all these conditions favor an increased rate of angiogenesis and eventual metastasis. Lactoferrin (Lf) is a mammalian iron-binding multifunctional glycoprotein regarded as a natural compound that up- and downregulates both humoral and cellular components of immunity involved in regulating the inflammatory response and maintaining gut homeostasis. Human and bovine Lf share high sequence homology and have very similar antimicrobial, anti-inflammatory, and immunomodulatory activities. Bovine Lf from milk is considered a safe molecule and is commercially available in large quantities. This review mainly focuses on the regulatory effects of orally administered bovine Lf on the inflammatory response associated with CRC; this approach indicates that CRC is one of the most frequently diagnosed cancers and affects the intestinal tract with high clinical and epidemiologic relevance. Thus, this review may provide foundations for the potential use of bovine Lf alone or as a natural adjunct agent to increase the effectiveness and reduce the side effects of anticancer chemotherapy.

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Section: Different Formulations Of Lactoferrin With Potential Activit...mentioning

confidence: 99%

Lactoferrin: A Nutraceutical with Activity against Colorectal Cancer

et al. 2022

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“…14 Aptamers, 15,16 short single-stranded DNAs or RNAs capable of binding specific targets through their tertiary structure, have gained considerable interest owing to their unique advantages, including easy chemical synthesis, high chemical stability, low molecular weight, low immunogenicity, low cost, and ease of modification. 17,18 Generally, aptamers are obtained from a random oligonucleotide library through the in vitro systematic evolution of ligands by the exponential enrichment (SELEX) process. [19][20][21] Most aptamers are screened using purified proteins, such as tumor markers and growth factors.…”

Section: Introductionmentioning

confidence: 99%

Selection of CD133-targeted DNA aptamers for the efficient and specific therapy of colorectal cancer

Wang

Gao

et al. 2022

J. Mater. Chem. B

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“…Consequently, research endeavors ought to be directed towards the improvement of diagnostic tools and the identification of therapeutic targets. In this sense, novel molecular tools such as aptamers have shown a high affinity and specificity for cancer detection and therapy for several cancer types including breast [6], cervical [7], colorectal [8], liver [9], lung [10], leukemia [11], and others.…”

Section: Introductionmentioning

confidence: 99%

Cell-Internalization SELEX of RNA Aptamers as a Starting Point for Prostate Cancer Research

Rodríguez-Dorantes

Cortés-Ramírez

Cruz-Burgos

et al. 2020

Methods in Molecular Biology

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In the treatment of cancer, over the last decade different drugs delivery systems have been developed to increase therapeutic specificity to improve drug's efficacy, and safety by increasing bioavailability. Among these systems, small nucleic acid molecules with a three-dimensional structure, known as aptamers, have shown several advantages. Several approaches to design aptamers require modifications from starting libraries of DNA sequences. Here, we describe cell-internalization SELEX (Systematic Evolution of Ligands by Exponential Enrichment), a sophisticated technique based on RNA aptamers as a starting point, that enables design functional aptamers as drug-delivery tools. This variation of the original SELEX technique using RNA aptamers instead DNA aptamers allows to obtain aptamers that are internalized in prostate cancer cells using as a starting point an RNA aptamer library with 76 nucleotides. The major advantage of this technique is that modifications are not required in the initial library, as initial T7 transcription promoter or 2 0 F nucleotides before sequencing.

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Nucleic acid aptamers in diagnosis of colorectal cancer

Cited by 25 publications

References 85 publications

Lactoferrin: A Nutraceutical with Activity against Colorectal Cancer

Lactoferrin: A Nutraceutical with Activity against Colorectal Cancer

Selection of CD133-targeted DNA aptamers for the efficient and specific therapy of colorectal cancer

Cell-Internalization SELEX of RNA Aptamers as a Starting Point for Prostate Cancer Research

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