Nucleic acid aptamer molecular recognition principles and application in liquid chromatography and capillary electrophoresis

Peyrin, Eric

doi:10.1002/jssc.200900061

Cited by 57 publications

(42 citation statements)

References 66 publications

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“…The specic conformational structures depend on properties of aptamer and environmental variables. 7,8 Identication and quantication of small molecules are essential for clinical diagnosis, environmental security, and food safety. stems, loops, bulges, hairpins, pseudoknots, triplexes, or quadruplexes) and molecular interactions (e.g.…”

Section: Introductionmentioning

confidence: 99%

Conformational structure-dependent molecular recognition of two aptamers for tetracycline

et al. 2015

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Different aptamers towards one target molecule can be selected bySystematic Evolution of Ligands by Exponential Enrichment (SELEX), however, not all aptamers have real world practicability. In this study, conformational structure-dependent molecular recognition of two aptamers towards tetracycline (TC), 76 mer and 40 mer, was studied both quantitatively and computationally. Two formats of competitive enzyme-linked aptamer assay (ELAA), a molecular docking module and Isothermal Titration Calorimeter (ITC) analysis were used to further investigate the two selected aptamers. With longer strand length, more G, C bases, and more recognition sites for TC, the 76 mer aptamer showed better performance than the 40 mer aptamer.Deciphering the relevance of aptamers with different molecular characteristics towards one target molecule can furnish as a referral guidance for aptamer selection and further practical application.

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Section: Introductionmentioning

confidence: 99%

Conformational structure-dependent molecular recognition of two aptamers for tetracycline

et al. 2015

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“…(50) As a result, sorbent materials with high affinity to their targets have been developed. In 2010, Stead et al (51) used a 38-mer consensus RNA sequence for Malachite green (MG) previously isolated by Grate and Wilson.…”

Section: Aptamersmentioning

confidence: 99%

Selective Nanoparticles in Microextraction

Ríos-Gómez

Lasarte‐Aragonés

Cárdenas

et al. 2016

Encyclopedia of Analytical Chemistry

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Sorption capacity is a critical issue in microextraction techniques where the amount of sorbent is reduced to the low milligram range and even lower. In this context, the use of sorbents with a high superficial area is really desirable. Nanoparticles (NPs) fulfill this main requirement while, at the same time, provide the analyst with a wide variety of interaction chemistries that can be easily selected depending on the analytical problem under study. In addition, certain NPs present especial properties (e.g. superparamagnetism) that make them so attractive in the microextraction context. Focusing on sorption capacity is meaningless when complex samples, the usual situation in Bioanalysis, are processed as matrix components may overload the sorbent avoiding the extraction of the target analytes. In this scenario, selectivity then becomes a critical variable of concern. This article discusses the role of selectivity in microextraction and provides a general overview of the main coatings used to boost the selectivity of NPs. In particular, this article describes the use of two groups of coatings as selectivity enhancers. On the one hand, the use of synthetic polymers including molecularly imprinted polymers (MIPs) and restricted access materials (RAMs) is a well‐established alternative in analytical sciences. On the other hand, biopolymers, such as antibodies and aptamers, exploit biorecognition that is by far the most selective interaction. Both alternatives are described in detail in this article.

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“…Upon binding of the target, the aptamer undergoes structural changes and the target is bound by an induced-fit mechanism [9]. The general molecular recognition properties are reviewed in Hermann and Patel (2000) and Peyrin (2009) [9,21]. The binding of the target is based on molecular recognition of complementary surfaces of the aptamer and the target.…”

Section: Binding Of the Targetmentioning

confidence: 99%

Aptamers as affinity ligands for downstream processing

Walter

Stahl

Scheper

2012

Engineering in Life Sciences

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Aptamers are single‐stranded synthetic oligonucleotides that are able to capture their target molecule with high affinity and specificity. Therefore, they can be thought of as nucleic acid‐based alternative to antibodies, which have several advantages over their amino acid‐based counterparts. Consequently, aptamers can be used in different applications based on molecular recognition including affinity separations. This review will summarize the state‐of‐the‐art in aptamer‐based affinity separations; will discuss the current limitations and will highlight possible future prospects. The first part will point out the advantages of aptamers in downstream processing. Here, the properties of aptamers will be discussed along with their implications on downstream processing from a user's point of view. In the second part, a brief summary of the literature is given with focus on aptamer‐based separation of proteins. Finally, some drawbacks of aptamers will be illustrated and possibilities to overcome these limitations will be suggested. New technologies in the fields of aptamer selection and synthesis are expected to further promote the use of aptamers as affinity ligands in downstream processing.

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Nucleic acid aptamer molecular recognition principles and application in liquid chromatography and capillary electrophoresis

Cited by 57 publications

References 66 publications

Conformational structure-dependent molecular recognition of two aptamers for tetracycline

Conformational structure-dependent molecular recognition of two aptamers for tetracycline

Selective Nanoparticles in Microextraction

Aptamers as affinity ligands for downstream processing

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