Nuclear transportation of exogenous epidermal growth factor receptor and androgen receptor via extracellular vesicles

Read, Jolene; Ingram, Alistair J.; Saleh, Hassan A. Al; Platko, Khrystyna; Gabriel, Kathleen; Kapoor, Anil; Pinthus, Jehonathan H.; Majeed, Fadwa; Qureshi, Talha; Al‐Nedawi, Khalid

doi:10.1016/j.ejca.2016.10.017

Cited by 53 publications

(51 citation statements)

References 45 publications

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“…Our data reveal a potential mechanism by which tumor cells modulate the level and activity of transcriptor factors in the nucleus of target cells via exosomes. A previous study reports the intercellular transport of nuclear receptor via extravesicles . This novel mechanism of intercellular communication leading to transcriptional regulation may contribute to an in‐depth understanding of the biology of CRC cells.…”

Section: Discussionmentioning

confidence: 74%

Colorectal cancer exosomes induce lymphatic network remodeling in lymph nodes

Sun

Zhou

Fang

et al. 2019

Intl Journal of Cancer

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The lymphatic network remodeling may guide tumor metastasis in a sentinel lymph node (SLN). Although tumor-derived exosomes have been demonstrated to modify the microenvironment in adjacent organs and initiate a premetastatic niche, their influence on the lymphatic network in SLNs has not been explained. Here, we show that CT26 cell exosomes (Exo) promote the proliferation of lymphatic endothelial cells and the formation of lymphatic network in SLN, facilitating the SLN metastasis of colorectal cancer (CRC). Uptake of Exo by macrophages promoted VEGFC secretion both in vivo and in vitro. Exo increased the frequency of F4/80 + macrophages in the SLN. Macrophage ablation by clodrosome prevented the exosomal effect on lymphatic network remodeling and SLN metastasis. Exosomal IRF-2 was highly expressed in serum exosomes isolated from CRC patients with LN metastasis relative to patients without LN metastasis and healthy controls. Mechanistically, exosomal IRF-2 induced the release of VEGFC by macrophages. An IRF-2 knockdown attenuated the lymphatic network remodeling in the SLN and suppressed the SLN metastasis. Our data suggest that exosomal IRF-2 remodels the lymphatic network in an SLN and may predict the development of CRC LN metastases.

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Section: Discussionmentioning

confidence: 74%

Colorectal cancer exosomes induce lymphatic network remodeling in lymph nodes

Sun

Zhou

Fang

et al. 2019

Intl Journal of Cancer

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“…Extracellular vesicles were collected from conditioned media for various cell lines and blood plasma as previously described . This study is supported by approval from the McMaster University research ethics board (REB # 13‐288‐T).…”

Section: Methodsmentioning

confidence: 99%

“…Extracellular vesicles were collected from conditioned media for various cell lines and blood plasma as previously described. 7,8 Protein bands were excised from the gel and subjected to MS-based proteomic analysis. Data searching and MS/MS spectra analysis were performed using PEAKS against the Human1302S database.…”

Section: Materials S and Me Thodsmentioning

confidence: 99%

The role of circulating extracellular vesicles in breast cancer classification and molecular subtyping

et al. 2019

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Currently, tumor biopsies are used for breast cancer molecular subtyping. Biopsies are associated with various pathological changes and are thought to contribute to the dissemination of tumor cells. Extracellular vesicles shed by tumor cells into circulation exhibit the molecular signature of the parent cells. Herein, we show that proteomic analysis of circulating EV can discriminate BC patients from healthy subjects and indicate stage of the disease. Also, we performed a correlation between the BC molecular subtype using plasma EV and immunohistochemistry of tumor biopsies. Circulating EV may represent a useful, non‐invasive tool to study the molecular makeup of BC tumors.

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“…DU145 cells were transfected to express EGFRvIII as described previously. 26 EGFRvIII expression in transfected Du145 cells is linked to a Tet-off system, 19 meaning the gene is expressed in the absence of, and blocked in the presence of tetracycline or doxycycline, which have not been used in this study. CHO cells were transfected to express the dual tagged PAR-1 using the same protocol used to generate EGFRvIII.…”

Section: Cell Transfectionmentioning

confidence: 99%

Thrombotic characteristics of extracellular vesicles derived from prostate cancer cells

et al. 2018

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The active oncogene EGFRvIII increases the concentration of TF and PAI-1 in EV. The procoagulant activity of EV is associated with the oncogenic and metastatic characteristics of their PC cells. Also, EV may contribute to the high procoagulant activity in the tumour microenvironment by the intercellular exchange of TF. Finally, through the generation of thrombin, EV can activate PAR-1, which evidently contributes to cancer progression, linking the coagulation system to tumor progression.

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Nuclear transportation of exogenous epidermal growth factor receptor and androgen receptor via extracellular vesicles

Cited by 53 publications

References 45 publications

Colorectal cancer exosomes induce lymphatic network remodeling in lymph nodes

Colorectal cancer exosomes induce lymphatic network remodeling in lymph nodes

The role of circulating extracellular vesicles in breast cancer classification and molecular subtyping

Thrombotic characteristics of extracellular vesicles derived from prostate cancer cells

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