Nuclear transplant studies of the determination of mating type in germ nuclei of Paramecium tetraurelia

Mikami, K.; Koizumi, Sadaaki

doi:10.1016/0014-4827(82)90041-6

Cited by 13 publications

(8 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…If the effect is transmitted through subsequent autogamies this result would confirm the presence of cytoplasmically localized determinants for E. He was unable to find an effect of transferring O cytoplasm into E cells. It was also reported (Koizumi and Kobayashi 1981, 1984; Koizumi, Kobayashi, and Mikami 1986; Mikami and Koizumi 1982) that macronucleoplasm transplanted from O cells to E cells transformed the E cells to O and that this was perpetuated through autogamy. In the case of transfer of nucleoplasm, transfer of the gene itself makes it impossible to distinguish the difference between genes and nucleoplasmic “factors”.…”

Section: Epigenetic Mechanisms In Macronuclear Developmentmentioning

confidence: 87%

“…Do the micronuclei have a role in epigenetic mating type determination? Evidence that they do was produced (Brygoo et al 1980; Sonneborn 1954) and the results were later confirmed (Mikami and Koizumi 1982). They were able to generate amicronucleate strains and crossed them with normal strains.…”

Section: Epigenetic Mechanisms In Macronuclear Developmentmentioning

confidence: 92%

“…Micronuclei have been transplanted into amicronucleate strains and then autogamy has been induced (Mikami and Koizumi 1982). In contrast to the results cited above they found no evidence for predetermination of the micronuclei.…”

Section: Epigenetic Mechanisms In Macronuclear Developmentmentioning

confidence: 99%

See 2 more Smart Citations

Epigenetic Mechanisms Affecting Macronuclear Development in Paramecium and Tetrahymena

Preer

2000

J Eukaryotic Microbiology

View full text Add to dashboard Cite

Epigenetic inheritance includes all non-Mendelian inheritance, in fact any inheritance that does not arise from base changes. Ciliates, particularly Paramecium and Tetrahymena, undergo epigenetic changes to their macronuclei when they are formed at nuclear reorganization. Once set, however, they are reproduced in a constant fashion, except for allelic segregations, during vegetative fissions in Tetrahymena and certain life cycle changes in both Paramecium and Tetrahymena. This review is meant to be inclusive, discussing all the known cases of epigenetic changes in macronuclei. They involve virtually all traits. We find that these macronuclear changes are subject to a variety of modifications in the way that they are implemented. They constitute a major feature of ciliate genetics, probably because the separation of generative and vegetative functions to micronuclei and macronuclei makes such changes possible.

show abstract

Section: Epigenetic Mechanisms In Macronuclear Developmentmentioning

confidence: 87%

Section: Epigenetic Mechanisms In Macronuclear Developmentmentioning

confidence: 92%

See 1 more Smart Citation

Epigenetic Mechanisms Affecting Macronuclear Development in Paramecium and Tetrahymena

Preer

2000

J Eukaryotic Microbiology

View full text Add to dashboard Cite

show abstract

“…It contains both micronuclear and macronuclear deletions of the A surface protein gene (13,15) as well as a complete macronuclear deletion of the B surface protein gene (17). It (9) and Mikami and Koizumi (12). Since P. tetraurelia contains two micronuclei, two steps of manipulation were necessary.…”

Section: Methodsmentioning

confidence: 99%

Non-Mendelian inheritance of macronuclear mutations is gene specific in Paramecium tetraurelia.

Scott¹,

Mikami²,

Leeck³

et al. 1994

Mol. Cell. Biol.

View full text Add to dashboard Cite

The ciliated protozoan Paramecium tetraurelia possesses two kinds of nuclei within a single cell. The highly polyploid macronucleus is transcriptionally active and therefore determines the phenotype of the cell. In contrast, the two diploid micronuclei are transcriptionally silent but participate in the nuclear reorganization events of autogamy (self-fertilization) and conjugation (reciprocal exchange of nuclei with cell of the opposite mating type). During either of these events, a new macronuclear genome is produced from the micronuclear genome through a process which involves extensive amplification and rearrangement of micronuclear DNA. This process of macronuclear development results in differences in the genetic content of the two types of nuclei.The previously isolated mutant d48 provides an example of how the macronuclear content in the Paramecium cell can differ from the micronuclear content.

show abstract

“…There are no previous reports to our knowledge of transformation in Paramecium using cloned or isolated genomic materials, although transfers of nucleoplasm have been shown to change several different characteristics (3)(4)(5). It has been reported (6, 7) that Stylonychia can be transformed to G418 resistance.…”

mentioning

confidence: 95%

Transformation of Paramecium by microinjection of a cloned serotype gene.

Godiska

Aufderheide

Gilley

et al. 1987

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Paramecia of a given serotype express only one of several possible surface proteins called immobilization antigens (i-antigens when the old macronucleus is replaced by a new one derived from the micronucleus. DNA from transformants contained the injected plasmid sequences, which were replicated within the paramecia. No evidence for integration was obtained. The majority of replicated plasmid DNA comigrated with a linearized form of the input plasmid. Nonetheless, the pattern of restriction fragments generated by transformant DNA and that generated by input plasmid DNA are identical and consistent with a circular rather than a linear map. These conflicting observations can be reconciled by assuming that a mixture of different linear fragments is present in the transformants, each derived from the circular plasmid by breakage at a different point. Copy-number determinations suggest the presence of 45,000-135,000 copies of the injected plasmid per transformed cell. These results suggest that the injected DNA contains information sufficient for both controlled expression and autonomous replication in Paramecium.

show abstract

Nuclear transplant studies of the determination of mating type in germ nuclei of Paramecium tetraurelia

Cited by 13 publications

References 8 publications

Epigenetic Mechanisms Affecting Macronuclear Development in Paramecium and Tetrahymena

Epigenetic Mechanisms Affecting Macronuclear Development in Paramecium and Tetrahymena

Non-Mendelian inheritance of macronuclear mutations is gene specific in Paramecium tetraurelia.

Transformation of Paramecium by microinjection of a cloned serotype gene.

Contact Info

Product

Resources

About