Nuclear Stress Bodies

Biamonti, Giuseppe; Vourc’h, Claire

doi:10.1101/cshperspect.a000695

Cited by 203 publications

(221 citation statements)

References 69 publications

(76 reference statements)

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Section: Resultsmentioning

confidence: 99%

“…Nuclear stress bodies (nSBs), another type of RNA-dependent nuclear body, occur in response to heat shock in a process initiated by the synthesis of lncRNA derived from pericentric tandem repeats of Satellite III (Sat III) sequences (22). Sat III lncRNA sequesters several splicing-related factors, such as serine/arginine-rich splicing factor 1 and scaffold attachment factor B (SAFB) (22). To determine whether SWI/SNF complexes are required for Sat III lncRNA-dependent formation of nSBs, the numbers of nSB-positive control HeLa cells, ΔBRG1/ ΔBRM HeLa cells, and SW13 cells lacking functional SWI/SNF complexes were determined before and after heat shock at 42°C for 1 h. The formation of nSBs in response to heat shock was impaired in ΔBRG1/ΔBRM HeLa cells and SW13 cells (Fig.…”

Section: Resultsmentioning

confidence: 99%

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SWI/SNF chromatin-remodeling complexes function in noncoding RNA-dependent assembly of nuclear bodies

Kawaguchi

Tanigawa²,

Naganuma

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

137

126

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Paraspeckles are subnuclear structures that form around nuclear paraspeckle assembly transcript 1 (NEAT1) long noncoding RNA (lncRNA). Recently, paraspeckles were shown to be functional nuclear bodies involved in stress responses and the development of specific organs. Paraspeckle formation is initiated by transcription of the NEAT1 chromosomal locus and proceeds in conjunction with NEAT1 lncRNA biogenesis and a subsequent assembly step involving >40 paraspeckle proteins (PSPs). In this study, subunits of SWItch/Sucrose NonFermentable (SWI/SNF) chromatin-remodeling complexes were identified as paraspeckle components that interact with PSPs and NEAT1 lncRNA. EM observations revealed that SWI/SNF complexes were enriched in paraspeckle subdomains depleted of chromatin. Knockdown of SWI/SNF components resulted in paraspeckle disintegration, but mutation of the ATPase domain of the catalytic subunit BRG1 did not affect paraspeckle integrity, indicating that the essential role of SWI/SNF complexes in paraspeckle formation does not require their canonical activity. Knockdown of SWI/SNF complexes barely affected the levels of known essential paraspeckle components, but markedly diminished the interactions between essential PSPs, suggesting that SWI/SNF complexes facilitate organization of the PSP interaction network required for intact paraspeckle assembly. The interactions between SWI/SNF components and essential PSPs were maintained in NEAT1-depleted cells, suggesting that SWI/SNF complexes not only facilitate interactions between PSPs, but also recruit PSPs during paraspeckle assembly. SWI/SNF complexes were also required for Satellite III lncRNA-dependent formation of nuclear stress bodies under heat-shock conditions. Our data suggest the existence of a common mechanism underlying the formation of lncRNA-dependent nuclear body architectures in mammalian cells.chromatin-remodeling complex | long noncoding RNA | nuclear bodies | ribonucleoprotein assembly

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

SWI/SNF chromatin-remodeling complexes function in noncoding RNA-dependent assembly of nuclear bodies

Kawaguchi

Tanigawa²,

Naganuma

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

137

126

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“…Later, Sarge et al [96] showed that HSF1 concentrates in these nSBs. Interestingly, nSBs seem to be limited to primate cells, where they can be induced by heat-shock, chemical and hypertonic stress (for reviews, see Jolly and Lakhotia [97]; Biamonti and Vourc'h [98]). …”

Section: Cell Stressmentioning

confidence: 99%

The increasing diversity of functions attributed to the SAFB family of RNA-/DNA-binding proteins

Norman

Rivers

Lee

et al. 2016

Biochemical Journal

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RNA-binding proteins play a central role in cellular metabolism by orchestrating the complex interactions of coding, structural and regulatory RNA species. The SAFB (scaffold attachment factor B) proteins (SAFB1, SAFB2 and SAFB-like transcriptional modulator, SLTM), which are highly conserved evolutionarily, were first identified on the basis of their ability to bind scaffold attachment region DNA elements, but attention has subsequently shifted to their RNA-binding and protein-protein interactions. Initial studies identified the involvement of these proteins in the cellular stress response and other aspects of gene regulation. More recently, the multifunctional capabilities of SAFB proteins have shown that they play crucial roles in DNA repair, processing of mRNA and regulatory RNA, as well as in interaction with chromatin-modifying complexes. With the advent of new techniques for identifying RNA-binding sites, enumeration of individual RNA targets has now begun. This review aims to summarise what is currently known about the functions of SAFB proteins.

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“…Alu and B2 SINE RNA ncRNAs bind to RNA polymerase II and inhibit its functional interaction with promoter DNA [56,57]. By contrast, satellite III ncRNAs promote the formation of nuclear stress bodies that rapidly effect changes to gene expression [58,59]. Nuclear stress bodies consist of transcriptional regulators and RNA processing factors, including the heat shock transcription factor 1, which promotes transcription of cytoprotective genes.…”

Section: Deployment Of Stress Responsesmentioning

confidence: 99%

Long Non-coding RNAs: Novel Targets for Nervous System Disease Diagnosis and Therapy

2013

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The human genome encodes tens of thousands of long non-coding RNAs (lncRNAs), a novel and important class of genes. Our knowledge of lncRNAs has grown exponentially since their discovery within the last decade. lncRNAs are expressed in a highly cell-and tissue-specific manner, and are particularly abundant within the nervous system. lncRNAs are subject to post-transcriptional processing and inter-and intra-cellular transport. lncRNAs act via a spectrum of molecular mechanisms leveraging their ability to engage in both sequence-specific and conformational interactions with diverse partners (DNA, RNA, and proteins). Because of their size, lncRNAs act in a modular fashion, bringing different macromolecules together within the three-dimensional context of the cell. lncRNAs thus coordinate the execution of transcriptional, post-transcriptional, and epigenetic processes and critical biological programs (growth and development, establishment of cell identity, and deployment of stress responses). Emerging data reveal that lncRNAs play vital roles in mediating the developmental complexity, cellular diversity, and activitydependent plasticity that are hallmarks of brain. Corresponding studies implicate these factors in brain aging and the pathophysiology of brain disorders, through evolving paradigms including the following: (i) genetic variation in lncRNA genes causes disease and influences susceptibility; (ii) epigenetic deregulation of lncRNAs genes is associated with disease; (iii) genomic context links lncRNA genes to disease genes and pathways; and (iv) lncRNAs are otherwise interconnected with known pathogenic mechanisms. Hence, Electronic supplementary material The online version of this article

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Nuclear Stress Bodies

Cited by 203 publications

References 69 publications

SWI/SNF chromatin-remodeling complexes function in noncoding RNA-dependent assembly of nuclear bodies

SWI/SNF chromatin-remodeling complexes function in noncoding RNA-dependent assembly of nuclear bodies

The increasing diversity of functions attributed to the SAFB family of RNA-/DNA-binding proteins

Long Non-coding RNAs: Novel Targets for Nervous System Disease Diagnosis and Therapy

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