Nuclear Size and Chromatin Concentration in Transitional Cell Carcinoma of the Human Urinary Bladder

Fosså, Sophie D.; Kaalhus, Olav

doi:10.1016/s0005-8165(76)80098-4

Cited by 29 publications

(11 citation statements)

References 30 publications

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“…These results are consistent with a previous study in which DNA, nuclear proteins and nuclear size increased in malignant cells [20,21,22,23]. Enlarged nuclei due to reactive changes may also appear in RM, with a small number of focus layers and few 3-dimensional properties (fig.…”

Section: Discussionsupporting

confidence: 93%

Discriminant Analysis between Malignant Mesothelioma and Reactive Mesothelium Using Nuclear Three-Dimensional Analysis Is Useful for Morphologically Suspicious Cases

et al. 2013

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Objective: Morphological discrimination between malignant mesothelioma (MM) and reactive mesothelium (RM) is often difficult. Stereological analysis of nuclear luminance using centrifuged smear samples from coelomic fluid and discriminant analysis based on Mahalanobis distance may help to more accurately discriminate between MM and RM. In the present study, discriminant analysis was conducted on cytological specimens using the auto-smear method in a blinded manner with regard to histological results. Study Design: Coelomic fluid samples of 28 cases, cytologically diagnosed using the auto-smear method, were analyzed to determine pixel counts, the number of focus layers, 3-dimensional variation in the coefficient of variation of nuclear luminance between the focus layers as well as roundness in about 30-50 atypical cell nuclei per case. These measurements were employed to determine malignancy based on Mahalanobis distance. Results: Discrimination rates were as high as 91.7% for MM and 82.7% for RM. The discrimination rates of MM with histology were >80% in 8 of 10 suspicious cases with the initial cytology. Conclusion: Our method allowed accurate discrimination between MM and RM and provides a useful alternative for the diagnosis of suspicious cases where morphological diagnosis of malignancy is difficult.

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Section: Discussionsupporting

confidence: 93%

Discriminant Analysis between Malignant Mesothelioma and Reactive Mesothelium Using Nuclear Three-Dimensional Analysis Is Useful for Morphologically Suspicious Cases

et al. 2013

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“…MM nuclei showed more 3D structures than RM nuclei (mean number of focus layers, 5.4 vs. 4.4), with the mean nuclear size being 1.7 times larger than that of RM. These results are consistent with the observation that DNA and nucleoprotein increase in cancer cells, resulting in enlarged nuclei [26,27,28,29]. In addition, the steric alterations in nuclear luminance (×10 –3 ) were smaller for MM (4.9 ± 3.6) than RM (10.7 ± 7.5).…”

Section: Discussionsupporting

confidence: 91%

Discriminant Analysis of Malignant Mesothelioma and Reactive Mesothelium Using Three-Dimensional Nuclear Estimation

et al. 2012

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Objective: The cytological diagnosis of coelomic fluid is essential for examining malignant mesothelioma (MM). However, reactive mesothelium (RM), caused by various factors, is morphologically similar to MM and thus often complicates the differential diagnosis. Here, nuclear luminance and steric alterations were assessed for the discriminant analysis of MM and RM. Study Design: Thirteen epithelial MM and 11 RM cases were included. One hundred alterations in the numbers of nuclear pixels and focus layers and the coefficient of variation of nuclear luminance among layers were determined for each case to conduct discriminant analysis using the Mahalanobis distance. Results: A cutoff value of 0.072 allowed highly accurate discrimination of MM (89.5%) and RM (89.6%). Fifteen cells appeared in 6 agglomerates of indiscriminable MM cases. The 6 agglomerates were individually examined. Malignant cells were dominant in 3 agglomerates (50%), allowing the discrimination of malignant cases. Conclusion: Discrimination using nuclear luminance and steric alterations is useful for morphologically indiscriminable MM cases. Three-dimensional analysis of agglomerates will be further investigated to improve the diagnostic accuracy.

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“…The results show that there is good correlation between the grade of malignancy and the DNA distribution pattern in urothelial bladder cancer (Levi et al, 1969;Fossa and Kaalhus, 1976 ; Tribukait et al, 1979; Gustafson et al, 1982a).…”

Section: Discussionmentioning

confidence: 93%

“…One of the most valuable aims of the method used, therefore, is the possibility of standardisation of histological grading. Fossa and Kaalhus (1976) used the same grading system (Bergkvist et al, 1965), and this represents a clearly defined schedule. However, there seems to be a marked difference between the grading method used by Fossa and Kaalhus (1976) and that used in our histopathological laboratory.…”

Section: Discussionmentioning

confidence: 99%

“…Fossa and Kaalhus (1976) used the same grading system (Bergkvist et al, 1965), and this represents a clearly defined schedule. However, there seems to be a marked difference between the grading method used by Fossa and Kaalhus (1976) and that used in our histopathological laboratory. Twelve of 30 grade I1 tumours in the study by Fossa and Kaalhus (1976) displayed nondiploid tumour stem lines, whereas nearly all of our tumours of the same malignancy group showed diploid stem lines.…”

Section: Discussionmentioning

confidence: 99%

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Biological Behaviour and DNA Cytophotometry of Urothelial Bladder Carcinoma

Hofstaedter

Jakse

Lederer

et al. 1984

British Journal of Urology

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In this prospective study, primary urothelial bladder tumour specimens from 64 patients were investigated by means of DNA Feulgen cytophotometry. The patients were followed clinically for at least 7 years. The objective cytophotometric parameters correlated well with the histopathological grading and the cytochemical data were of considerable prognostic value. They were closely correlated with the stage of the tumour, 5- and 7-year survival rates and the probability of tumour recurrence. It is concluded that DNA cytophotometry can serve as a method for the standardisation of histological grading and can give more objective and more detailed prognostic information than conventional histopathology.

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Nuclear Size and Chromatin Concentration in Transitional Cell Carcinoma of the Human Urinary Bladder

Cited by 29 publications

References 30 publications

Discriminant Analysis between Malignant Mesothelioma and Reactive Mesothelium Using Nuclear Three-Dimensional Analysis Is Useful for Morphologically Suspicious Cases

Discriminant Analysis between Malignant Mesothelioma and Reactive Mesothelium Using Nuclear Three-Dimensional Analysis Is Useful for Morphologically Suspicious Cases

Discriminant Analysis of Malignant Mesothelioma and Reactive Mesothelium Using Three-Dimensional Nuclear Estimation

Biological Behaviour and DNA Cytophotometry of Urothelial Bladder Carcinoma

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