Nuclear receptor NR5A2 is involved in the calreticulin gene regulation during renal fibrosis

Arvaniti, Eleni; Vakrakou, Athina; Kaltezioti, Valeria; Στεργιόπουλος, Αθανάσιος; Prakoura, Niki; Politis, Panagiotis K.; Charonis, Aristidis S.

doi:10.1016/j.bbadis.2016.06.013

Cited by 8 publications

(13 citation statements)

References 34 publications

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“…Interestingly, many of the individual proteins we found to be differentially abundant between CKD-DS and CKD-RS rats were previously identified in other reports on CKD and kidney associated diseases [16, 30, 32, 33, 37, 38]. Importantly, changes in these proteins were previously identified either in plasma or in urine.…”

Section: Resultssupporting

confidence: 62%

Metaproteomics reveals potential mechanisms by which dietary resistant starch supplementation attenuates chronic kidney disease progression in rats

et al. 2019

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BackgroundResistant starch is a prebiotic metabolized by the gut bacteria. It has been shown to attenuate chronic kidney disease (CKD) progression in rats. Previous studies employed taxonomic analysis using 16S rRNA sequencing and untargeted metabolomics profiling. Here we expand these studies by metaproteomics, gaining new insight into the host-microbiome interaction.MethodsDifferences between cecum contents in CKD rats fed a diet containing resistant starch with those fed a diet containing digestible starch were examined by comparative metaproteomics analysis. Taxonomic information was obtained using unique protein sequences. Our methodology results in quantitative data covering both host and bacterial proteins.Results5,834 proteins were quantified, with 947 proteins originating from the host organism. Taxonomic information derived from metaproteomics data surpassed previous 16S RNA analysis, and reached species resolutions for moderately abundant taxonomic groups. In particular, the Ruminococcaceae family becomes well resolved–with butyrate producers and amylolytic species such as R. bromii clearly visible and significantly higher while fibrolytic species such as R. flavefaciens are significantly lower with resistant starch feeding. The observed changes in protein patterns are consistent with fiber-associated improvement in CKD phenotype. Several known host CKD-associated proteins and biomarkers of impaired kidney function were significantly reduced with resistant starch supplementation. Data are available via ProteomeXchange with identifier PXD008845.ConclusionsMetaproteomics analysis of cecum contents of CKD rats with and without resistant starch supplementation reveals changes within gut microbiota at unprecedented resolution, providing both functional and taxonomic information. Proteins and organisms differentially abundant with RS supplementation point toward a shift from mucin degraders to butyrate producers.

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Section: Resultssupporting

confidence: 62%

Metaproteomics reveals potential mechanisms by which dietary resistant starch supplementation attenuates chronic kidney disease progression in rats

et al. 2019

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“…Interestingly, many of the individual proteins we found to be differentially abundant between CKD-DS and CKD-RS rats were previously identified in other reports on CKD and kidney associated diseases (16,24,26,27,31,32). Importantly, these proteins were identified either in plasma or in urine.…”

Section: Rat Proteinssupporting

confidence: 56%

Metaproteomics Reveals Potential Mechanisms by which Dietary Resistant Starch Supplementation Attenuates Chronic Kidney Disease Progression in Rats

Zybaylov

Glazko

Rahmatallah

et al. 2018

Preprint

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“…For example, Cpd3 was used to identify Calreticulin as a novel LRH-1 target gene, thus establishing a role for LRH-1 during renal fibrosis. 161 The first in vivo application of Cpd3 in zebrafish embryos demonstrated that LRH-1 is essentially involved in multiple stages of liver and pancreas organogenesis. 162 Confirming a vital role for LRH-1 in cell proliferation, SR1848 treatment was shown to suppress the cell division activity of primary murine granulosa cells ex vivo.…”

Section: Potential Of Lrh-1 Targeting Approaches In Cancer Therapymentioning

confidence: 99%

“…Thus, these small molecule LRH‐1 antagonists have been successfully used in various studies to investigate LRH‐1‐regulated processes. For example, Cpd3 was used to identify Calreticulin as a novel LRH‐1 target gene, thus establishing a role for LRH‐1 during renal fibrosis 161 . The first in vivo application of Cpd3 in zebrafish embryos demonstrated that LRH‐1 is essentially involved in multiple stages of liver and pancreas organogenesis 162 .…”

Section: Potential Of Lrh‐1 Targeting Approaches In Cancer Therapymentioning

confidence: 99%

Nuclear‐mitochondrial crosstalk: On the role of the nuclear receptor liver receptor homolog‐1 (NR5A2) in the regulation of mitochondrial metabolism, cell survival, and cancer

Michalek

Brunner

2020

IUBMB Life

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Liver receptor homolog-1 (LRH-1, NR5A2) is an orphan nuclear receptor with widespread activities in the regulation of development, stemness, metabolism, steroidogenesis, and proliferation. Many of the LRH-1-regulated processes target the mitochondria and associated activities. While under physiological conditions, a balanced LRH-1 expression and regulation contribute to the maintenance of a physiological equilibrium, deregulation of LRH-1 has been associated with inflammation and cancer. In this review, we discuss the role and mechanism(s) of how LRH-1 regulates metabolic processes, cell survival, and cancer in a nuclear-mitochondrial crosstalk, and evaluate its potential as a pharmacological target.

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Nuclear receptor NR5A2 is involved in the calreticulin gene regulation during renal fibrosis

Abstract: These data establish for the first time a role for NR5A2 and its SUMOylation on the transcriptional regulation of the calreticulin gene in a rodent model of renal fibrosis and raise the possibility that NR5A2 might be a novel target for future anti-fibrotic interventions.

Cited by 8 publications

References 34 publications

Metaproteomics reveals potential mechanisms by which dietary resistant starch supplementation attenuates chronic kidney disease progression in rats

Metaproteomics reveals potential mechanisms by which dietary resistant starch supplementation attenuates chronic kidney disease progression in rats

Metaproteomics Reveals Potential Mechanisms by which Dietary Resistant Starch Supplementation Attenuates Chronic Kidney Disease Progression in Rats

Nuclear‐mitochondrial crosstalk: On the role of the nuclear receptor liver receptor homolog‐1 (NR5A2) in the regulation of mitochondrial metabolism, cell survival, and cancer

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