Nuclear receptor HR3 controls locust molt by regulating chitin synthesis and degradation genes of Locusta migratoria

Zhao, Xiaoming; Qin, Zhongyu; Liu, Weimin; Liu, Xiaojian; Moussian, Bernard; Ma, Enbo; Li, Sheng; Zhang, Jianzhen

doi:10.1016/j.ibmb.2017.11.001

Cited by 65 publications

(52 citation statements)

References 52 publications

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“…RT‐qPCR showed the mRNA level of LmHR39 was significantly down‐regulated when LmEcR mRNA levels were depleted after injection of ds LmEcR for 48 h compared with that of the control (Figs B, S1A). We previously reported that the transcripts of LmHR3 are induced by 20E in vivo and were significantly suppressed by successful knock down of the expression of LmEcR (Zhao et al ., ). Thus, we hypothesized that LmHR39 might respond to the LmEcR‐LmHR3‐mediated 20E signalling pathway.…”

Section: Resultsmentioning

confidence: 97%

“…First, the cuticle begins to undergo apolysis, and then the old cuticle is degraded; meanwhile, the new cuticle is gradually synthesized. These processes are governed by 20E, which binds to its receptor (EcR/USP) and activates the expression of a series of nuclear transcription factors, such as E75, HR3, HR4 and FTZ‐F1 (Carney et al ., ; Broadus et al ., ; Li et al ., ; Xu et al ., ; Zhao et al ., ). The two different FTZ‐F1 proteins, betaFTZ‐F1 and FTZ‐F1beta (HR39), are encoded by the Drosophila genome (Ohno et al ., ).…”

Section: Discussionmentioning

confidence: 97%

“…Surprisingly, the chitin synthesis pathway gene, UAP1 , was up‐regulated in the transcriptome data, whereas the expression of CHS1 did not change (Fig. C), which is different from the regulation mediated by LmHR3 (Zhao et al ., ). It seems that HR3 and LmHR39 mediated different regulatory pathways in the chitin synthesis pathway.…”

Section: Discussionmentioning

confidence: 97%

“…The quality and quantity of total RNA were evaluated on 1.5% agarose gels and using a NanoDrop 2000 (Thermo Fisher, Waltham, MA, USA). The synthesis of cDNA and RT‐qPCR analysis were performed following previously described methods (Zhao et al ., ). Relative mRNA levels of target genes were calculated using the 2 −∆∆Ct method by normalizing to the expression of the internal marker gene ribosomal protein L32 , which exhibited the most stable expression at different stages and in different tissues (Livak and Schmittgen, ; Yang et al ., ).…”

Section: Methodsmentioning

confidence: 97%

“…Chitin is the main component of the insect cuticle, and its synthesis and degradation genes respond to regulation of the 20E signalling pathway (Yao et al ., ; Li et al ., ; Zhang and Zheng, ; Liu et al ., ). Our previous study verified that LmHR3 is involved in the moulting of Locusta migratoria by regulating the chitin synthesis and degradation genes (Zhao et al ., ). However, whether the LmHR39‐mediated 20E signalling pathway regulates locust moulting remains unclear.…”

Section: Introductionmentioning

confidence: 97%

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Nuclear receptor hormone receptor 39 is required for locust moulting by regulating the chitinase and carboxypeptidase genes

Zhao

Qin

Zhang

et al. 2019

Insect Molecular Biology

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The nuclear receptor‐mediated 20‐hydroxyecdysone (20E) signalling pathway plays crucial roles in insects by initiating and regulating moulting and metamorphosis. In the present study, we identified and characterized a cDNA encoding a putative nuclear receptor protein (Locusta migratoria hormone receptor 39, LmHR39) based on L. migratoria transcriptomics data. Reverse‐transcription quantitative PCR (RT‐qPCR) revealed that LmHR39 shows low‐level expression in the early days of fifth‐instar nymphs, and peak expression occurs on day 5, which is followed by a decrease before ecdysis. LmHR39 transcription could be induced by 20E in vivo and was significantly suppressed by knocking down the expression of the L. migratoria ecdysone receptor gene and early‐late gene LmHR3. After RNA interference of LmHR39 with double‐stranded RNA (dsRNA), 85% of the insects showed abnormal morphology, with curly wings after moulting and delayed eclosion time. Haematoxylin and eosin staining indicated that apolysis of the integument and wing pad cuticle in the dsLmHR39‐treated insects was delayed compared to that in the dsRNA for green fluorescent protein‐injected control. Furthermore, RNA‐sequencing and RT‐qPCR analysis showed the expression level of carboxypeptidase genes (Carboxypeptidase A (CPA) and Carboxypeptidase M (CPM)) and chitin degrading genes (LmChitinase5 (LmCHT5) and LmChitinase10 (LmCHT10)) dramatically declined in the dsLmHR39‐treated insects, implying that the LmHR39‐mediated 20E signalling pathway is involved in the regulation of carboxypeptidase genes (CPA and CPM) and chitinase genes (LmCHT5 and LmCHT10), and participated in apolysis of the integument and wing pads during locust moulting.

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Section: Resultsmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 97%

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Nuclear receptor hormone receptor 39 is required for locust moulting by regulating the chitinase and carboxypeptidase genes

Zhao

Qin

Zhang

et al. 2019

Insect Molecular Biology

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Functional analysis of nuclear receptor HR96 gene in Bombyx mori exposed to phoxim

Liu

et al. 2022

Arch Insect Biochem Physiol

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The nuclear receptor (NRs) gene family functions as liganddependent transcription factors in a variety of animals, which participates in a variety of biological processes, such as cell differentiation, metabolic regulation, reproduction, development, insect metamorphosis. In this study, a nuclear receptor HR96 gene in silkworm Bombyx mori (BmHR96) was identified, and the responses of BmHR96 gene to 20-hydroxyecdysone (20E), three insecticides, and two disinfectants were analyzed and its function in phoxim exposure was explored. Quantitative real-time polymerase chain reaction indicated that the expression of BmHR96 mRNA was the highest in ovary of 5th instar Day 3 silkworm larvae and in silk gland of the wandering stage. The expression patterns of BmHR96 gene in ovary, head, testis, and midgut of different stages were different. After injecting 20E into B. mori, the expression of BmHR96 mRNA had no significant difference compared with control. Three insecticides and two disinfectants were used to treat B. mori, respectively, and it was found that they had different influence patterns on the expression level of BmHR96. siRNA of BmHR96 was injected into silkworm larvae and the expression of BmHR96 was decreased significantly after injecting 72 h. After silencing of BmHR96, B. mori was fed with phoxim-treated leaves. The results showed that the mortality of B. mori after silencing of BmHR96 was significantly higher than the control. Our results indicated

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Genome‐wide identification and analysis of nuclear receptors genes for lethal screening against Bemisia tabaci Q

Liu

Liang

et al. 2020

Pest Management Science

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BACKGROUND: Nuclear receptors (NRs) play an essential role in diverse biological processes, such as insect metamorphosis. Here, transcriptome analysis and functional studies were used to determine whether NRs are involved in metamorphosis of whitefly Bemisia tabaci Q, a serious pest to crops, and to find some potential insecticide targets.RESULTS: Twenty NRs were identified in the Bemisia tabaci Q genome and categorized into the NR0-NR6 subfamilies. The phylogenetic tree of NRs from Bemisia tabaci Q and other representative species was constructed, which provided evolutionary insight into their genetic distances. The results of spatiotemporal gene expression indicated that the majority of NR gene expression was higher in the head than the abdomen and higher in eggs than adults. Further functional analysis using RNA interference (RNAi) showed that NR genes play an important role in Bemisia tabaci Q pupation and eclosion. With respect to high mortality and effects on growth, this was reflected in the unable to become pupa when the third-stage nymph treated with double-stranded RNA (dsRNA) and the developmental time delay (4-7 days) when pupae were treated with dsRNA for the 12 NR genes during molting compared with the development time in the control.CONCLUSION: This study provides insight into NR functions during the metamorphosis stages of Bemisia tabaci Q. Several candidate genes could be potential insecticide targets for whitefly pest control due to their important roles in insect development.

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Nuclear receptor HR3 controls locust molt by regulating chitin synthesis and degradation genes of Locusta migratoria

Cited by 65 publications

References 52 publications

Nuclear receptor hormone receptor 39 is required for locust moulting by regulating the chitinase and carboxypeptidase genes

Nuclear receptor hormone receptor 39 is required for locust moulting by regulating the chitinase and carboxypeptidase genes

Functional analysis of nuclear receptor HR96 gene in Bombyx mori exposed to phoxim

Genome‐wide identification and analysis of nuclear receptors genes for lethal screening against Bemisia tabaci Q

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