Nuclear protein(s) binding to the conserved DNA hexameric sequence postulated to regulate transcription of wheat histone genes

Mikami, Koji; Tabata, Tetsuya; Kawata, Takefumi; Nakayama, Takuya; Iwabuchi, Masaki

doi:10.1016/0014-5793(87)80303-4

Cited by 81 publications

(50 citation statements)

References 25 publications

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“…In addition to these motifs, there is also a pentanucleotide (TGACG) repeat located between -82 to -78 and -70 to -66 ( Figure 3). This pentanucleotide is highly homologous to the hexamer motif identified upstream of histone genes by Iwabuchi and co-workers (Mikami et al, 1987). The relative importance of these sequence motifs to the 35S activity is being evaluated.…”

Section: The -90 To -46 Region Plays An Accessory Role In Transcriptionmentioning

confidence: 89%

Multiple cis regulatory elements for maximal expression of the cauliflower mosaic virus 35S promoter in transgenic plants.

et al. 1989

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The 35S promoter is a major promoter of the cauliflower mosaic virus that infects crucifers. This promoter is still active when excised from cauliflower mosaic virus and integrated into the nuclear genome of transgenic tobacco. Previous work has shown that the -343 to -46 upstream fragment is responsible for the majority of the 35S promoter strength (Odell, J.T., Nagy, F., and Chua, N.-H. [1985]. Nature 313, 810-812). Here we show by 5', 3', and internal deletions that this upstream fragment can be subdivided into three functional regions, -343 to -208, -208 to -90, and -90 to -46. The first two regions can potentiate transcriptional activity when tested with the appropriate 35S promoter sequence. In contrast, the -90 to -46 region by itself has little activity but it plays an accessory role by increasing transcriptional activity of the two distal regions. Finally, we show that monomers and multimers of a 35S fragment (-209 to -46) can act as enhancers to potentiate transcription from a heterologous promoter.

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Section: The -90 To -46 Region Plays An Accessory Role In Transcriptionmentioning

confidence: 89%

Multiple cis regulatory elements for maximal expression of the cauliflower mosaic virus 35S promoter in transgenic plants.

et al. 1989

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“…The basic region is implicated in DNA binding and the leucine zipper mediates homo-and heterodimerization. Several plant bZIP proteins have been shown to bind the ACGT cis-acting element identified in the promoters of bacterial, viral, and plant genes (Mikami et al 1987;Giuliano et al 1988;Bouchez et al 1989;Lam et al 1989;Schindler et al 1992aSchindler et al , 1992b. Based on their DNA-binding specificity and heterodimerization characteristics, these plant bZIP proteins can be classified into at least two classes: the G box (CCACGTGG)-binding factor (GBF) family and the TGACGT/C-binding proteins (Schindler et al 1992a;Foster et al 1994).…”

Section: Pan Encodes a Bzip Proteinmentioning

confidence: 99%

The PERIANTHIA gene encodes a bZIP protein involved in the determination of floral organ number in Arabidopsis thaliana

Chuang¹,

Running²,

Williams³

et al. 1999

Genes & Development

198

145

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Mutations in the PERIANTHIA (PAN) gene of Arabidopsis thaliana specifically transform flowers from tetramerous to largely pentamerous, which is a characteristic of flowers of ancestral plants. We have cloned the PAN gene and here we show that it encodes a member of the basic region/leucine zipper class of transcription factors. Immunohistochemical analysis shows that the encoded protein is present in the apical meristem, the floral meristem, each whorl of organ primordia, and in ovule primordia during wild-type flower development. PAN expression occurs independently of genes affecting floral meristem identity, floral meristem size, or floral organ number. The near absence of a phenotype in transgenic plants overexpressing PAN and the contrast between the broad expression of PAN and the specificity of its mutant phenotype suggest that its activity may be regulated post-translationally or by the presence of partner proteins. Based on these results and on data reported previously, we propose models for the role of PAN in the evolution of flower pattern in the mustard family. Arabidopsis flowers arise initially as undifferentiated bulges on the flank of the apical meristem. Soon afterward, whorls of organ primordia arise sequentially. Four sepal primordia develop along the edges of the floral meristem, establishing the first whorl. Then four petal and six stamen primordia initiate in whorls 2 and 3, respectively, followed by development of two carpel primordia in the center of the floral meristem (Smyth et al. 1990).A proposed mechanism of floral organ primordium initiation and formation must account for how a primordium is formed and also when and where on the floral meristem it develops. The eventual fate of organ primordia is determined by the organ identity genes (Komaki et al. 1988;Bowman et al. 1989Bowman et al. , 1991Bowman et al. , 1993Hill and Lord 1989;Kunst et al. 1989;Irish and Sussex 1990;Jack et al. 1992;Weigel and Meyerowitz 1994); however, the position in which they arise (and thus their number) appears to be established independently (Meyerowitz 1997). How proper floral organ number is achieved is not well understood.One way to understand the molecular mechanisms by which appropriate floral organ number is determined is to find mutations that affect floral organ number but do not affect floral organ identity or floral meristem identity, and then to elucidate the nature and the function of the corresponding wild-type genes. There are a number of reports demonstrating the existence of such muta-, and wiggum (wig) (Leyser and Furner 1992;Clark et al. 1993Clark et al. , 1995Clark et al. , 1997Roe et al. 1993Roe et al. , 1997Sessions and Zambryski 1995;Talbert et al. 1995;Running and Meyerowitz 1996;Sessions et al. 1997;Laufs et al. 1998;Kayes and Clark 1998;Running et al. 1998). In clv1, clv3, and wig mutants, the increase in organ number is correlated with increased cell number in the floral meristem (Clark et al. 1993(Clark et al. , 1995Running et al. 1998). This indicates a mechanism by which the pos...

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“…The as-1 element is able to confer expression primarily in root tissues, but also interacts synergistically with other cis-elements (Lam et al, 1989) and has been shown to bind AS1 tobacco nuclear factor (Fromm et al, 1989). This region also contains a sequence, ACGTCA, with homology to the hexamer motif of plant histone promoters (Mikami et al, 1987). The presence of this motif in the genomes of several other ssDNA plant viruses has been suggested to reflect an evolutionary conservation of transcription control mechanisms within this group (Morozov et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

Promoter activity associated with the intergenic regions of banana bunchy top virus DNA-1 to -6 in transgenic tobacco and banana cells.

Dugdale

Beetham

Becker

et al. 1998

Journal of General Virology

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Promoter regions associated with each of the six ssDNA components of banana bunchy top virus (BBTV) have been characterized. DNA segments incorporating the intergenic regions of BBTV DNA-1 to -6 were isolated and fused to the uidA (β-glucuronidase) reporter gene to assess promoter activity. In tobacco cell suspensions, the BBTV DNA-2 and -6 promoters generated levels of GUS expression 2-fold greater and similar to the 800 bp CaMV 35S promoter, respectively. Deletion analysis of the BBTV DNA-6 promoter suggested all the necessary promoter elements required for strong expression were located within 239 nucleotides upstream of the translational start codon. In transgenic tobacco plants, the BBTV-derived promoters generally provided a weak, tissue-specific GUS

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Nuclear protein(s) binding to the conserved DNA hexameric sequence postulated to regulate transcription of wheat histone genes

Cited by 81 publications

References 25 publications

Multiple cis regulatory elements for maximal expression of the cauliflower mosaic virus 35S promoter in transgenic plants.

Multiple cis regulatory elements for maximal expression of the cauliflower mosaic virus 35S promoter in transgenic plants.

The PERIANTHIA gene encodes a bZIP protein involved in the determination of floral organ number in Arabidopsis thaliana

Promoter activity associated with the intergenic regions of banana bunchy top virus DNA-1 to -6 in transgenic tobacco and banana cells.

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