Nuclear Pore Complexes: “Press-Stud” Elements of Chromosomes in Pairing and Control

Engelhardt, Peter; Pusa, K.

doi:10.1038/newbio240163a0

Cited by 51 publications

(19 citation statements)

References 42 publications

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“…Some authors have suggested that the chromatin fibrils arc attached to the inner part of the annulus (Claude 1964;DuPraw 1965DuPraw , 1968DuPraw , 1970Comings & Okada 1970a;Engelhardt & Pusa 1972;La Cour & Wells 1972;Sorsa 1972). The inner annular granules would then constitute the attachment points of the deoxyribonucleoproteins (DNP) of the chromatin illament (Comings 1968;DuPraw 1968;Comings & Okada 1970b;Engelhardt & Pusa 1972;Sorsa 1972).…”

Section: Introductionmentioning

confidence: 99%

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Superchromosomal organization and its cytogenetic consequences in the Eukaryota

Chiarelli

Brøgger²

1978

Genetica

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Section: Introductionmentioning

confidence: 99%

“…The inner annular granules would then constitute the attachment points of the deoxyribonucleoproteins (DNP) of the chromatin illament (Comings 1968;DuPraw 1968;Comings & Okada 1970b;Engelhardt & Pusa 1972;Sorsa 1972). Convincing evidence for this has not yet been found.…”

Section: Introductionmentioning

confidence: 99%

Superchromosomal organization and its cytogenetic consequences in the Eukaryota

Chiarelli

Brøgger²

1978

Genetica

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“…6-8 and 17). However, the PCs appear to occur in association with the synaptonemal complexes (14,18,19) and inside the nucleus (18,20). Thorough inspection of the NM with negative staining and scanning (20,21) and stereo (22) electron microscopy reveals a number of particles similar to annular granules of the PCs which may be involved in the biogenesis of the latter (20,21).…”

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confidence: 99%

Granules 25-30 nm in diameter: basic constituent of the nuclear matrix, chromosome scaffold, and nuclear envelope.

Engelhardt¹,

Plagens²,

Zbarsky³

et al. 1982

Proc. Natl. Acad. Sci. U.S.A.

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Rat liver nuclear matrix and similar structures derived from isolated Chironomus polytene chromosomes, nuclear envelopes, and intranuclear bodies of frog late oocytes (the karyospheres) were studied by electron microscopy with platinum shadowing and negative staining. We have shown that the treatment of whole nuclei, nuclear envelopes, polytene chromosomes, or karyospheres with nonionic detergent, high salt, and RNase and DNase followed by dilute alkali or hyaluronidase digestion reveals numerous rather uniform granules 25-30 nm in diameter. With omission ofthe nucleases the granules appear to be associated with DNA strands mostly organized in loops. Many granules form clusters and are arranged in linear or arch-like aggregates or cycles resembling the pore complexes. We suppose that these spherical bodies constitute a basic component of the nuclear matrix, chromosome scaffold, and nuclear envelope and are bound together by hyaluronic acid or some similar glycosaminoglycan.When isolated nuclei are extracted with a high-salt solution, the chromatin is removed and a nonhistone residue remains; most ofthe residue is soluble in dilute alkali (1). Nuclei extracted with high-salt solution consist of residual nucleoli, intranuclear ribonucleoprotein network, and remnants of the nuclear enve-

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“…The blurring and brush-like images observed in the periphery of the nuclei after moderate digestions may be the result of the enzyme induced disruption of the nuclear envelope with the subsequent protrusion of the chromatin. It is well known that the chromatin is attached to multiple sites of the nuclear envelope by mean of structures called nu clear-pore complexes which are resistant to various types of disruptive treatments (Engelhardt and Pusa 1972). Accordingly, it seems reasonable to assume that filaments forming the brush-like image may be composed of large groups of chro matin fibers protruding out after the disruption of the nuclear membrane and still attached to their corresponding nuclear-pore complexes.…”

Section: Discussionmentioning

confidence: 99%