Nuclear pore complex acetylation regulates <scp>mRNA</scp> export and cell cycle commitment in budding yeast

Gomar-Alba, Mercè; Pozharskaia, Vasilisa; Cichocki, B.; Schaal, Celia; Kumar, Arun; Jacquel, Basile; Igual, J. Carlos; Mendoza, Manuel

doi:10.15252/embj.2021110271

Cited by 5 publications

(4 citation statements)

References 78 publications

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“…Their general structure and part of their components remain conserved from yeast to humans, although recent evidence suggests that some elements vary substantially between species [ 11 , 13 , 14 ]. Far from being mere support structures, they have been shown to be involved in processes such as alternative DNA recombination/repair, recombination-mediated telomere elongation, and gene expression regulation, while also acting as regulatory hubs for the coordination of transcript elongation, processing, and export [ 15 , 16 , 17 ].…”

Section: The Yeast Nucleus: a Model To Address Nuclear Morphology And...mentioning

confidence: 99%

A Yeast Mitotic Tale for the Nucleus and the Vacuoles to Embrace

Santana-Sosa

Matos-Perdomo

Ayra-Plasencia

et al. 2023

IJMS

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The morphology of the nucleus is roughly spherical in most eukaryotic cells. However, this organelle shape needs to change as the cell travels through narrow intercellular spaces during cell migration and during cell division in organisms that undergo closed mitosis, i.e., without dismantling the nuclear envelope, such as yeast. In addition, the nuclear morphology is often modified under stress and in pathological conditions, being a hallmark of cancer and senescent cells. Thus, understanding nuclear morphological dynamics is of uttermost importance, as pathways and proteins involved in nuclear shaping can be targeted in anticancer, antiaging, and antifungal therapies. Here, we review how and why the nuclear shape changes during mitotic blocks in yeast, introducing novel data that associate these changes with both the nucleolus and the vacuole. Altogether, these findings suggest a close relationship between the nucleolar domain of the nucleus and the autophagic organelle, which we also discuss here. Encouragingly, recent evidence in tumor cell lines has linked aberrant nuclear morphology to defects in lysosomal function.

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Section: The Yeast Nucleus: a Model To Address Nuclear Morphology And...mentioning

confidence: 99%

A Yeast Mitotic Tale for the Nucleus and the Vacuoles to Embrace

Santana-Sosa

Matos-Perdomo

Ayra-Plasencia

et al. 2023

IJMS

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“…In Saccharomyces cerevisiae , HOS3 plays an important role in life span (Pang et al., 2022 ). HOS3 specifically associates with nuclear pore complexes in daughter cells during mitosis and deacetylates Nup60 in G1 daughter cells, thus delaying cell cycle entry to restrain their premature commitment to a new cell division cycle (Gomar‐Alba et al., 2022 ; Kumar et al., 2018 ). Yeast HOS3 has an intrinsic HDAC activity and a distinct specificity to H2AK7, H2BK11, H3K14, H3K23, H4K5 and H4K8 in vitro.…”

Section: Introductionmentioning

confidence: 99%

UvHOS3‐mediated histone deacetylation is essential for virulence and negatively regulates ustilaginoidin biosynthesis in Ustilaginoidea virens

Wang,

Duan,

Liu

et al. 2024

Molecular Plant Pathology

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Ustilaginoidea virens is the causal agent of rice false smut, which has recently become one of the most important rice diseases worldwide. Ustilaginoidins, a major type of mycotoxins produced in false smut balls, greatly deteriorates grain quality. Histone acetylation and deacetylation are involved in regulating secondary metabolism in fungi. However, little is yet known on the functions of histone deacetylases (HDACs) in virulence and mycotoxin biosynthesis in U. virens. Here, we characterized the functions of the HDAC UvHOS3 in U. virens. The ΔUvhos3 deletion mutant exhibited the phenotypes of retarded growth, increased mycelial branches and reduced conidiation and virulence. The ΔUvhos3 mutants were more sensitive to sorbitol, sodium dodecyl sulphate and oxidative stress/H2O2. ΔUvhos3 generated significantly more ustilaginoidins. RNA‐Seq and metabolomics analyses also revealed that UvHOS3 is a key negative player in regulating secondary metabolism, especially mycotoxin biosynthesis. Notably, UvHOS3 mediates deacetylation of H3 and H4 at H3K9, H3K18, H3K27 and H4K8 residues. Chromatin immunoprecipitation assays indicated that UvHOS3 regulates mycotoxin biosynthesis, particularly for ustilaginoidin and sorbicillinoid production, by modulating the acetylation level of H3K18. Collectively, this study deepens the understanding of molecular mechanisms of the HDAC UvHOS3 in regulating virulence and mycotoxin biosynthesis in phytopathogenic fungi.

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“…Indeed, RNA-seq measures RNA steady-state abundance, which depends on both RNA synthesis and degradation rates. Notably, the budding yeast homologue of TIP60 has been shown to promote both the synthesis and nuclear export of mRNA (Gomar-Alba et al, 2022), suggesting that TIP60 may also regulate gene expression post-transcriptionally. Therefore, characterisation of multiple steps in RNA metabolism is essential to understand the role of TIP60 in gene expression regulation.…”

Section: Introductionmentioning

confidence: 99%

Gene-specific RNA homeostasis revealed by perturbation of coactivator complexes

Forouzanfar,

Plassard,

Furst

et al. 2024

Preprint

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Transcript buffering entails the reciprocal modulation of mRNA synthesis and degradation rates, ensuring a constant RNA concentration amidst changes in cellular conditions. While an increasing body of research supports a global, non-sequence-specific linkage between mRNA synthesis and degradation, the underlying mechanisms remain elusive. To explore this, we investigated alterations in RNA metabolism following the acute depletion of TIP60/KAT5, the transcriptional coactivator and acetyltransferase subunit of the NuA4 complex, in mouse embryonic stem cells. By combining RNA sequencing of nuclear, cytoplasmic, and newly synthesised transcript fractions with biophysical modelling, we show that TIP60 activates transcription of numerous genes, with substantially fewer genes undergoing transcriptional repression. Surprisingly, specific RNA species' transcription changes triggered by TIP60 depletion were counterbalanced by compensatory adjustments in RNA export and/or stability within the nucleus and in RNA stability within the cytoplasm. These discoveries imply that transcript buffering operates on a gene-specific level and suggest that cells continually monitor RNA molecule counts in nuclear and cytoplasmic compartments to maintain cellular homeostasis.

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Nuclear pore complex acetylation regulates mRNA export and cell cycle commitment in budding yeast

Cited by 5 publications

References 78 publications

A Yeast Mitotic Tale for the Nucleus and the Vacuoles to Embrace

A Yeast Mitotic Tale for the Nucleus and the Vacuoles to Embrace

UvHOS3‐mediated histone deacetylation is essential for virulence and negatively regulates ustilaginoidin biosynthesis in Ustilaginoidea virens

Gene-specific RNA homeostasis revealed by perturbation of coactivator complexes

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