Nuclear Organisation of Immunoglobulin Genes

Skok, Jane; Brown, K; Azuara, Véronique; Caparros, Marta; Baxter, Jeff; Takács, Krisztina; Dillon, Niall; Gray, David; Perry, Robert P.; Merkenschlager, Matthias; Fisher, A.G.

doi:10.1100/tsw.2002.909

Cited by 1 publication

(2 citation statements)

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“…Probes were directly labeled by nick translation with dUTP-Cy3 or dUTP-A488 (Invitrogen). The g-satellite probe (Skok et al, 2001) was labeled with dUTP-Cy5 (GE Healthcare). Cells were analyzed by confocal microscopy on a Leica Sp5 AOBS (Acoustica Optical Beam Splitter) system.…”

Section: Immunoblottingmentioning

confidence: 99%

“…Three-Dimensional DNA FISH Three-dimensional DNA-FISH experiments were performed as previously described (Skok et al, 2001) The Igk DNA probes were generated from BACs 101G13 (Vk24) and 387E13 (Igk constant region) in combination with a g-satellite probe. Probes were directly labeled by nick translation with dUTP-Cy3 or dUTP-A488 (Invitrogen).…”

Section: Immunoblottingmentioning

confidence: 99%

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Regulation of Immunoglobulin Light-Chain Recombination by the Transcription Factor IRF-4 and the Attenuation of Interleukin-7 Signaling

et al. 2008

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Productive rearrangement of the immunoglobulin heavy-chain locus triggers a major developmental checkpoint that promotes limited clonal expansion of pre-B cells, thereby culminating in cell-cycle arrest and rearrangement of light-chain loci. By using Irf4-/-Irf8-/- pre-B cells, we demonstrated that two pathways converge to synergistically drive light-chain rearrangement, but not simply as a consequence of cell-cycle exit. One pathway was directly dependent on transcription factor IRF-4, whose expression was elevated by pre-B cell receptor signaling. IRF-4 targeted the immunoglobulin 3'Ekappa and Elambda enhancers and positioned a kappa allele away from pericentromeric heterochromatin. The other pathway was triggered by attenuation of IL-7 signaling and activated the iEkappa enhancer via binding of the transcription factor E2A. IRF-4 also regulated expression of chemokine receptor Cxcr4 and promoted migration of pre-B cells in response to the chemokine ligand CXCL12. We propose that IRF-4 coordinates the two pathways regulating light-chain recombination by positioning pre-B cells away from IL-7-expressing stromal cells.

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Section: Immunoblottingmentioning

confidence: 99%

Section: Immunoblottingmentioning

confidence: 99%