Nuclear mutants of yeast with reduced spontaneous mutability of the mitochondrial genome

Devin, A. B.; Koltovaya, N. A.

doi:10.1016/0165-7992(81)90051-8

Cited by 11 publications

(12 citation statements)

References 6 publications

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“…Devin and Koltovaya (66) began with a strain which produced 50% petite mutants and selected four nuclear mutations giving rise to a low level of petites (1% in three cases and 9% in the fourth). The four mutations are recessive and map to different complementation groups.…”

Section: No Compromise: Nuclear Mutants Killed By Mtdna Instability Amentioning

confidence: 99%

Maintenance and Integrity of the Mitochondrial Genome: a Plethora of Nuclear Genes in the Budding Yeast

Contamine

Picard

2000

Microbiol Mol Biol Rev

262

209

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SUMMARY Instability of the mitochondrial genome (mtDNA) is a general problem from yeasts to humans. However, its genetic control is not well documented except in the yeast Saccharomyces cerevisiae. From the discovery, 50 years ago, of the petite mutants by Ephrussi and his coworkers, it has been shown that more than 100 nuclear genes directly or indirectly influence the fate of the rho+ mtDNA. It is not surprising that mutations in genes involved in mtDNA metabolism (replication, repair, and recombination) can cause a complete loss of mtDNA (rho0 petites) and/or lead to truncated forms (rho−) of this genome. However, most loss-of-function mutations which increase yeast mtDNA instability act indirectly: they lie in genes controlling functions as diverse as mitochondrial translation, ATP synthase, iron homeostasis, fatty acid metabolism, mitochondrial morphology, and so on. In a few cases it has been shown that gene overexpression increases the levels of petite mutants. Mutations in other genes are lethal in the absence of a functional mtDNA and thus convert this petite-positive yeast into a petite-negative form: petite cells cannot be recovered in these genetic contexts. Most of the data are explained if one assumes that the maintenance of the rho+ genome depends on a centromere-like structure dispensable for the maintenance of rho− mtDNA and/or the function of mitochondrially encoded ATP synthase subunits, especially ATP6. In fact, the real challenge for the next 50 years will be to assemble the pieces of this puzzle by using yeast and to use complementary models, especially in strict aerobes.

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Section: No Compromise: Nuclear Mutants Killed By Mtdna Instability Amentioning

confidence: 99%

Maintenance and Integrity of the Mitochondrial Genome: a Plethora of Nuclear Genes in the Budding Yeast

Contamine

Picard

2000

Microbiol Mol Biol Rev

262

209

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“…No interactions between CDC28 and any of the aforementioned genes involved in feedback controls have been revealed so far (Weinert and Hartwell, 1993;Weinert et al, 1994). We have found that certain gene mutations in S. cerevisiae including cdc28-srm affect maintenance of different (and differently compartmentalized) genetic structures at once (Devin and Koltovaya, 1981;Devin et al, 1990). The cdc28-srm was accompanied by decreased mitotic stability of excess natural chromosomes and recombinant circular plasmids containing centromeres as well as decreased rates of spontaneous mitochondrial rho mutations (Devin et al, 1990).…”

Section: Cdc28mentioning

confidence: 90%

“…srm1 per se, like some other srm mutations, determines decreased spontaneous rho mutability (Devin and Koltovaya, 1981) without any appreciable effect on the cell growth or radiation sensitivity (Table 4, and Figure 1A and B). Double srm1 cdc28-srm mutants were less rho mutable than single srm1 or cdc28-srm mutants (Devin et al, 1990).…”

Section: Interaction Between Cdc28-srm and Srm1 Mutationsmentioning

confidence: 92%

“…Nuclear gene mutations designated as mmg and then redesignated as srm were accompanied by decreased spontaneous rho mutability (Devin and Koltovaya, 1981;Devin et al, 1990). Monospore clones derived from 71a cdc28-1, cdc28-srm cdc28-13, and cdc28-srm cdc28-1 hybrids were used to characterize the effects of the cdc28 mutations on the spontaneous rho mutagenesis at 30 C. As seen from Table 3, rho mutagenesis was differently affected by different cdc28 mutations.…”

Section: Effects Of Cdc28 Mutations On Spontaneous Rho Mutagenesismentioning

confidence: 99%

“…The yeast strains constructed in this work carry mutations srm1 (Devin and Koltovaya, 1981), cdc28-srm (Devin et al, 1990), and other gene mutations (their designations are given in parentheses), derived from strains 185-3-4 (cdc28-1), g160/2b (rad52-1) and …”

Section: Strains and Plasmidsmentioning

confidence: 99%

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Mutations of theCDC28 gene and the radiation sensitivity ofSaccharomyces cerevisiae

Koltovaya

Arman

Devin

1998

Yeast

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CDC28‐srm, a non‐temperature‐sensitive (ts) mutation in the CDC28 gene of Saccharomyces cerevisiae that affects fidelity of mitotic transmission of both mitochondrial and nuclear genetic structures (Devin et al., 1990), also affected cell growth and sensitivity to lethal effects of ionizing radiation. At 30°C CDC28‐13, a ts mutation, was without appreciable effects on spontaneous mitochondrial rho−‐mutagenesis, cell growth and radiation sensitivity, whereas all three cell characteristics mentioned were affected (although to a lesser degree than by CDC28‐srm) by CDC28‐1, another ts mutation. CDC28‐srm was without any significant effect on the rates of spontaneous nuclear gene mutations and γ‐ray‐induced mitotic recombination. An analysis of double mutants as regards their radiation sensitivity has revealed additive or even synergistic interactions between the CDC28‐srm mutation and every one of the rad6‐1 and rad52‐1 mutations. The rad9Δ allele was found to be epistatic to CDC28‐srm. These data suggest that the p34CDC28 protein is involved in the RAD9‐dependent feedback control of DNA integrity operating at the cell cycle checkpoints. © 1998 John Wiley & Sons, Ltd.

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NET1 and HFI1 genes of yeast mediate both chromosome maintenance and mitochondrial rho⁻ mutagenesis

Koltovaya

Guerasimova

Tchekhouta

et al. 2003

Yeast

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An increase in the mitochondrial rho − mutagenesis is a well-known response of yeast cells to mutations in numerous nuclear genes as well as to various kinds of stress. Despite extensive studies for several decades, the biological significance of this response is still not fully understood. The genetic approach to solving this enigma includes a study of genes that are required for the high incidence of spontaneous rho − mutants. We have obtained mutations of a few nuclear genes of that sort and found that mutations in certain genes, including CDC28, the central cell-cycle regulation gene, result in a decrease in spontaneous rho − mutability and simultaneously affect the maintenance of the yeast chromosomes and plasmids. Two more genes resembling CDC28 in this respect are identified in the present work as a result of the characterization of four new mutants. These two genes are NET1 and HFI1 which mediate important regulatory protein-protein interactions in the yeast cell. The effects of four mutations, including net1-srm and hfi1-srm, on the maintenance of the yeast mitochondrial genome, chromosomes and plasmids, as well as on the cell's sensitivity to ionizing radiation, are also described. The data presented suggest that the pleiotropic srm mutations determining coordinate changes in the fidelity of mitotic transmission of chromosomes, plasmids and mtDNA molecules identify genes that most probably operate high up in the hierarchy of the general genetic regulation of yeast.

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Nuclear mutants of yeast with reduced spontaneous mutability of the mitochondrial genome

Cited by 11 publications

References 6 publications

Maintenance and Integrity of the Mitochondrial Genome: a Plethora of Nuclear Genes in the Budding Yeast

Maintenance and Integrity of the Mitochondrial Genome: a Plethora of Nuclear Genes in the Budding Yeast

Mutations of theCDC28 gene and the radiation sensitivity ofSaccharomyces cerevisiae

NET1 and HFI1 genes of yeast mediate both chromosome maintenance and mitochondrial rho⁻ mutagenesis

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Nuclear mutants of yeast with reduced spontaneous mutability of the mitochondrial genome

Cited by 11 publications

References 6 publications

Maintenance and Integrity of the Mitochondrial Genome: a Plethora of Nuclear Genes in the Budding Yeast

Maintenance and Integrity of the Mitochondrial Genome: a Plethora of Nuclear Genes in the Budding Yeast

Mutations of theCDC28 gene and the radiation sensitivity ofSaccharomyces cerevisiae

NET1 and HFI1 genes of yeast mediate both chromosome maintenance and mitochondrial rho− mutagenesis

Contact Info

Product

Resources

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NET1 and HFI1 genes of yeast mediate both chromosome maintenance and mitochondrial rho⁻ mutagenesis