Nuclear MMP‐9 role in the regulation of rat skeletal myoblasts proliferation

Zimowska, Małgorzata; Swierczynska, Marta M.; Ciemerych, Maria A.

doi:10.1111/boc.201300020

Cited by 16 publications

(16 citation statements)

References 46 publications

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“…Our previous analyses of selected aspects of crushinduced regeneration, 15,16,34,35,45 as well as in vitro differentiation of satellite cells isolated from EDL and soleus muscles 15,46,47 extensively documented existing differences. For example, focusing at the myogenic regulatory factors (MRFs), i.e., MyoD, Myf-5, MRF4, and myogenin, we have shown that they are expressed earlier during fast-twitch EDL muscle repair as compared to slow-twitch soleus muscle.…”

Section: Discussionmentioning

confidence: 99%

Inflammatory response during slow- and fast-twitch muscle regeneration

et al. 2016

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Section: Discussionmentioning

confidence: 99%

Inflammatory response during slow- and fast-twitch muscle regeneration

et al. 2016

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“…MMP-9 is involved in various physiological functions, such as tissue remodeling, cellular differentiation (Zimowska et al, 2013), cell-cell contact and cell migration (Kim et al, 2012), the release of cytokines and regulation of growth factor activity (Schonbeck et al, 1998), survival and apoptosis (Kowluru et al, 2011), angiogenesis, inflammation, and signaling (for a comprehensive review of MMP-9 function, see Vandooren et al, 2013; Verslegers et al, 2013). Aside from maintaining tissue homeostasis, MMP-9 plays a role in a range of pathologies (comprehensively reviewed in Rivera et al, 2010; Kaczmarek, 2013).…”

Section: Matrix Metalloproteinase-9mentioning

confidence: 99%

Matrix metalloproteinase-9 involvement in the structural plasticity of dendritic spines

2014

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Dendritic spines are the locus for excitatory synaptic transmission in the brain and thus play a major role in neuronal plasticity. The ability to alter synaptic connections includes volumetric changes in dendritic spines that are driven by scaffolds created by the extracellular matrix (ECM). Here, we review the effects of the proteolytic activity of ECM proteases in physiological and pathological structural plasticity. We use matrix metalloproteinase-9 (MMP-9) as an example of an ECM modifier that has recently emerged as a key molecule in regulating the morphology and dysmorphology of dendritic spines that underlie synaptic plasticity and neurological disorders, respectively. We summarize the influence of MMP-9 on the dynamic remodeling of the ECM via the cleavage of extracellular substrates. We discuss its role in the formation, modification, and maintenance of dendritic spines in learning and memory. Finally, we review research that implicates MMP-9 in aberrant synaptic plasticity and spine dysmorphology in neurological disorders, with a focus on morphological abnormalities of dendritic protrusions that are associated with epilepsy.

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“…The presence of nuclear MMP9 has been documented in several cell types, including human neuroblastoma cells, human endothelial cells (Sinha et al ., ), rodent myoblasts (Zimowska et al ., ) and murine macrophages (Marchant et al ., ). The nuclear MMP9 signal in spermatogonia and cells around the seminiferous epithelium, as illustrated in this study, may be associated with processes such as mitosis that require nuclear matrix degradation, a proposal that requires further investigation.…”

Section: Discussionmentioning

confidence: 99%

Activin A target genes are differentially expressed between normal and neoplastic adult human testes: clues to gonocyte fate choice

et al. 2018

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Background Human testicular germ cell tumours (TGCT) arise from germ cell neoplasia in situ (GCNIS) cells that originate from foetal germ cell precursors. Activin A is central to normal foetal testis development, and its dysregulation may contribute to TGCT aetiology. Objective (i) To test whether the expression profiles of activin A targets in normal and neoplastic human testes indicates functional links with TGCT progression. (ii) To investigate whether activin A levels influence MMP activity in a neoplastic germ cell line. Materials and Methods (1) Bouin's fixed, paraffin‐embedded human testes were utilized for PCR‐based transcript analysis and immunohistochemistry. Samples (n = 5 per group) contained the following: (i) normal spermatogenesis, (ii) GCNIS or (iii) seminoma. CXCL12, CCL17, MMP2 and MMP9 were investigated. (2) The human seminoma‐derived TCam‐2 cell line was exposed to activin A (24 h), and target transcripts were measured by qRT‐PCR (n = 4). ELISA (n = 4) and gelatin zymography (n = 3) showed changes in protein level and enzyme activity, respectively. Results (i) Cytoplasmic CXCL12 was detected in Sertoli and other somatic cells, including those surrounding seminoma cells. Anti‐CCL17 labelled only the cytoplasm of Sertoli cells surrounding GCNIS, while anti‐MMP2 and anti‐MMP9 labelled germline and epithelial‐like cells in normal and neoplastic testes. (ii) Exposing TCam‐2 cells to activin A (50 ng/mL) elevated MMP2 and MMP9 transcripts (fourfold and 30‐fold), while only MMP2 protein levels were significantly higher after activin A (5 ng/mL and 50 ng/mL) exposure. Importantly, gelatin zymography revealed activin A increased production of activated MMP2. Discussion Detection of CCL17 only in GCNIS tumours may reflect a change in Sertoli cell phenotype to a less mature state. Stimulation of MMP2 activity by activin A in TCam‐2 cells suggests activin influences TGCT by modulating the tumour niche. Conclusion This knowledge provides a basis for understanding how physiological changes that influence activin/TGF‐β superfamily signalling may alter germ cell fate.

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Nuclear MMP‐9 role in the regulation of rat skeletal myoblasts proliferation

Abstract: During myoblasts differentiation, MMP-9 that is localised in nuclei might be involved in the processes regulating cell cycle progression.

Cited by 16 publications

References 46 publications

Inflammatory response during slow- and fast-twitch muscle regeneration

Inflammatory response during slow- and fast-twitch muscle regeneration

Matrix metalloproteinase-9 involvement in the structural plasticity of dendritic spines

Activin A target genes are differentially expressed between normal and neoplastic adult human testes: clues to gonocyte fate choice

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