Nuclear magnetic resonance study of lecithin-cholesterol interactions

Darke, A.; Finer, E.G.; Flook, A.G.; Phillips, Michael C.

doi:10.1016/0022-2836(72)90374-9

Cited by 236 publications

(95 citation statements)

References 37 publications

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“…lipid fatty acyl chains in the presence of cholesterol [2,3]. Moreover, using differential scanning calorimetry a decrease of order has been detected in the gel phase [4].…”

Section: Ln~mentioning

confidence: 99%

Effect of cholesterol on membrane microheterogeneity: a study using 1,6-diphenyl-1,3,5-hexatriene fluorescence lifetime distributions

Fiorini

Gratton

Curatola

1989

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metaboli

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“…lipid fatty acyl chains in the presence of cholesterol [2,3]. Moreover, using differential scanning calorimetry a decrease of order has been detected in the gel phase [4].…”

Section: Ln~mentioning

confidence: 99%

Effect of cholesterol on membrane microheterogeneity: a study using 1,6-diphenyl-1,3,5-hexatriene fluorescence lifetime distributions

Fiorini

Gratton

Curatola

1989

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metaboli

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“…It was recently suggested [5][6][7] that in bilayers of phosphatidylcholine and cholesterol a phase separation occurs at low cholesterol concentrations in that clusters of cholesterol-phosphatidylcholine complexes are present next to free phosphatidylcholine. If this lateral phase separation is comparable with that demonstrated in a mixture of phosphatidylcholines that shows monotectic behaviour this should be able to be visualized with freeze fracturing.…”

Section: The Influence Of Cholesterol On the Crystallization Behavioumentioning

confidence: 99%

The distribution of cholesterol in bilayers of phosphatidylcholines as visualized by freeze fracturing

Verkleij

Ververgaert

Kruyff

et al. 1974

Biochimica et Biophysica Acta (BBA) - Biomembranes

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SUMMARYThe crystallization behaviour of bilayers of synthetic phosphatidylcholines in the presence of cholesterol was investigated by freeze fracturing.I. Below the lipid-phase transition, cholesterol is randomly distributed over the lateral plane of a bilayer of the single species dimyristoylphosphatidylcholine, as far as can be detected within the lateral resolution of freeze fracturing.2. in an equimolar mixture of dilauroyl-and dimyristoylphosphatidylcholine, which shows cocrystallization, cholesterol is distributed randomly in the bilayers below the phase-transition temperature.3. In an equimolar mixture of dipalmitoyl and l-palmitoyl-2-oleoylphosphatidylcholine, which shows monotectic behaviour, cholesterol interacts preferentially with the liquid crystalline species 1-palmitoyl-2-oleoylphosphatidylcholine when the other component dipalmitoylphosphatidylcholine passes from the liquid crystalline to the gel state upon cooling the mixture.

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“…Spectroscopic data reveal that cholesterol restricts the thermal motion of fatty acid chains in a phospholipid bilayer above the gel-to-liquid transition temperature (14)(15)(16), and this causes a "condensation effect" and decreases permeability (17). An increase in bilayer microviscosity evidently accounts for the inhibit'ry effect of cholesterol on fusion between plasma membranes of cultured muscle cells (18) and synthetic phospholipid membranes (19).…”

mentioning

confidence: 99%

Studies on the mechanism of capacitation: albumin-mediated changes in plasma membrane lipids during in vitro incubation of rat sperm cells.

Davis¹,

Byrne²,

Bedigian³

1980

Proc. Natl. Acad. Sci. U.S.A.

145

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Plasma membrane isolated from rat sperm cells after incubation in vitro had a significantly lower cholesterol/phospholipid mole ratio when the medium contained serum albumin. Transfer of albumin-bound phospholipids to the membrane can largely account for this effect. The result is broadly consistent with a previously proposed model for albumin-induced destabilization of sperm membrane (capacitation) and its reversal by seminal plasma membrane vesicles. Albumin also decreased sialic acid and, more specifically, ganglioside levels, presumably by promoting release of sperm neuraminidase. Cholesteryl ester comprised up to 0.5 mol/mol of cholesterol in these plasma mem rane preparations. Mammalian sperm cells can express fertilizing capacity after incubation in vitro for an interval of usually a few hours in a chemically defined medium containing serum albumin (1-6). Although the molecular changes underlying transformation to a capacitated state are unclear, albumin specifically facilitates this process (4). Substantial changes in sperm plasma membrane proteins accompany incubation with albumin (7); however, they seem to involve proteolysis after the acrosome reaction. A clue that lipid binding by albumin might be involved in its ability to promote capacitation was provided by the observation that presaturation of the protein with cholesterolt abolished this capability (4). In view of this finding and the discovery that cholesterol-bearing decapitation factor (DF) vesicles from seminal plasma and synthetic vesicles containing the sterol block capacitation in rabbit and rat spermatozoa (8-12), changes in the lipid bilayer of the sperm plasma membrane seem to be necessary for expression of fertilizing capacity.One interpretation of these observations is that albumin decreases the cholesterol/phospholipid (Chol/PL) ratio in the sperm plasma membrane (13). Spectroscopic data reveal that cholesterol restricts the thermal motion of fatty acid chains in a phospholipid bilayer above the gel-to-liquid transition temperature (14-16), and this causes a "condensation effect" and decreases permeability (17). An increase in bilayer microviscosity evidently accounts for the inhibit'ry effect of cholesterol on fusion between plasma membranes of cultured muscle cells (18) and synthetic phospholipid membranes (19). Decreasing the Chol/PL ratio in sperm plasma mqmbrane, therefore, may be anticipated to promote fusion with the outer acrosomal membrane; this intracellular fusion occurring in the acrosome reaction is a precondition for mammalian fertilization (20). A vesicle-induced reversal of this putative change in the plasma membrane Chol/PL ratio might account for the ability of these vesicles to inhibit spermatozoan fertilizing capacity reversibly (11,12).To test this idea, plasma membrane preparations isolated from rat spermatozoa after incubation in vitro were partially characterized with respect to their lipid composition. It was anticipated bovine serum albumin would decrease the Chol/PL ratio in sperm plasma membrane i...

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Nuclear magnetic resonance study of lecithin-cholesterol interactions

Cited by 236 publications

References 37 publications

Effect of cholesterol on membrane microheterogeneity: a study using 1,6-diphenyl-1,3,5-hexatriene fluorescence lifetime distributions

Effect of cholesterol on membrane microheterogeneity: a study using 1,6-diphenyl-1,3,5-hexatriene fluorescence lifetime distributions

The distribution of cholesterol in bilayers of phosphatidylcholines as visualized by freeze fracturing

Studies on the mechanism of capacitation: albumin-mediated changes in plasma membrane lipids during in vitro incubation of rat sperm cells.

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