Nuclear Magnetic Resonance Studies of Fracture Repair

Newman, Raymond J.; Duthie, Robert B.; Francis, M. J. O.

doi:10.1097/00003086-198509000-00044

Cited by 20 publications

(26 citation statements)

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“…Their method caused serious damage to the soft tissues surrounding the fracture. In contrast to Newman et al [17], who noted the rats were sick for 2 days following the fracture procedure, we observed the rats walking around on three legs, lifting the fractured leg, after only 1 day of convales cence. This may be explained by the lesser trauma to the soft tissues produced in our model.…”

Section: Discussioncontrasting

confidence: 99%

“…In the few studies that used a closed fracture model of the rat tibia the fracture was often produced by means of manual force [16] which in our opinion is difficult to control and reproduce in a standard manner. Newman et al [17] produced a closed tibial fracture with a crushing pair of pliers. Their method caused serious damage to the soft tissues surrounding the fracture.…”

Section: Discussionmentioning

confidence: 99%

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Closed Fracture Healing: A Rat Model

Patka

Haarman³

1995

Eur Surg Res

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A rat model for the study of closed fracture healing is described. Standard closed tibial fractures were produced with specially designed pliers in 24 rats. All fractures were located in the middle third of the tibia and healed without complications as delayed union, infection or soft tissue problems. The average angle of the fracture line with the axis of the tibia on lateral view was 80 ± 2° (SEM). The model is considered to be suited for the study of closed fracture healing processes. Closed intramedullary fracture fixation, can also be evaluated in this model.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Closed Fracture Healing: A Rat Model

Patka

Haarman³

1995

Eur Surg Res

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“…The acid tide of wound healing, i.e., the pH within wounds and fractures, change from acidic to neutral and alkaline pH as the healing process progress (18)(19)(20)(21)(22)(23)(24). The local environment is acidic during the initial stage of healing.…”

Section: Discussionmentioning

confidence: 99%

“…Previously, it has also been described that mineralization and bone repair is pH dependent. The early acidity within fracture hematomas is later followed by a change in pH towards alkalinity (19)(20)(21)(22)(23)(24). A low pH has also been reported during the early phase of wound healing, hence designated the acid tide (25).…”

Section: Introductionmentioning

confidence: 99%

Variation of pH in lysed platelet concentrates influence proliferation and alkaline phosphatase activity in human osteoblast-like cells

et al. 2007

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Interestingly, we also found BMP-2 in platelet lysate. However BMP-2 was only present in LPB pH 4.0. We suggest, based on these experimental findings, that acidic milieu influence platelets to release growth factors more potent to stimulate osteoblast proliferation than neutral and alkaline platelet preparations. Lysed platelet concentrates prepared at an alkaline pH might release additional components with stimulating effects pH of platelet concentrate and growth stimulation 4 resulting in other features than cell proliferation. This is the first report, to our knowledge, about the presence of BMP-2 in platelet lysate and about a pH dependent stimulatory effect of lysed platelet concentrates on human osteoblast-like cell proliferation. Lysed platelet concentrates, preincubated in acidic or alkaline buffers, may benefit fracture healing, implant fixation and might also be advantageous in the treatment of wounds with platelet constituents; however, this has to be investigated in extended experimental and clinical settings.pH of platelet concentrate and growth stimulation 5

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“…During wound healing, relatively acidic conditions prevail. 42,43 Low blood flow and poor oxygenation can result in a decreased ability to nourish bone cells and remove metabolic waste products such as lactic acid. 27,44 Conversely, patients with respiratory alkalosis have an increased rate of fracture healing.…”

Section: Discussionmentioning

confidence: 99%

Effects of pH on human bone marrow stromal cells in vitro: Implications for tissue engineering of bone

Kohn

Sarmadi

Helman

et al. 2002

J. Biomed. Mater. Res.

130

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The objective of this study was to address the hypothesis that changes in extracellular pH alter collagen gene expression, collagen synthesis, and alkaline phosphatase activity in bone marrow stromal cells (BMSCs). Potential effects of pH on cell function are of particular importance for tissue engineering because considerable effort is being placed on engineering biodegradable polymers that may generate a local acidic microenvironment on degradation. Human and murine single-cell marrow suspensions were plated at a density of 2 x 10(4) cells/cm(2). After 7 days in culture, the pH of the culture medium was adjusted to one of six ranges: > or = 7.8, 7.5.-7.7, 7.2-7.4, 6.9-7.1, 6.6-6.8, or < or = 6.5. After 48 h of exposure to an altered pH, alkaline phosphatase activity and collagen synthesis decreased significantly with decreasing pH. This decrease was two-to threefold as pH decreased from 7.5 to 6.6. In contrast, alpha1(I) procollagen mRNA levels increased two- to threefold as pH was decreased. The trend in osteocalcin mRNA expression was opposite to that of collagen. Small shifts in extracellular pH led to significant changes in the ability of BMSCs to express markers of the osteoblast phenotype. These pH effects potentially relate to the microenvironment supplied by a tissue-engineering scaffold and suggest that degrading polymer scaffolds may influence the biologic activity of the cells in the immediate environment.

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Nuclear Magnetic Resonance Studies of Fracture Repair

Cited by 20 publications

References 0 publications

Closed Fracture Healing: A Rat Model

Closed Fracture Healing: A Rat Model

Variation of pH in lysed platelet concentrates influence proliferation and alkaline phosphatase activity in human osteoblast-like cells

Effects of pH on human bone marrow stromal cells in vitro: Implications for tissue engineering of bone

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