Nuclear magnetic resonance studies of polysaccharide structure and interactions

Casu, Benito

doi:10.1007/978-1-349-06369-7_1

Cited by 8 publications

(8 citation statements)

References 144 publications

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“…Such a shift has also been observed in other polysaccharides when substitution with sulphated groups occurred. 19 The signals relative to glucosamine (69É9 ppm), C 4 and glucuronic (73É0 ppm) and (74É1 ppm) show C 2 C 3 little variation of chemical shifts or of intensity, which indicates that a partial substitution may involve these positions.…”

Section: Sulphated Hyal Derivativesmentioning

confidence: 98%

Sulphated hyaluronic acids: a chemical and biological characterisation

et al. 1998

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Section: Sulphated Hyal Derivativesmentioning

confidence: 98%

Sulphated hyaluronic acids: a chemical and biological characterisation

et al. 1998

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“…1) of this fraction corresponded to that of a carbohydrate structure that had no nonsugar substituents and only one kind of anomeric proton. The chemical shift (4.9 ppm) of the signal that arose from the anomeric proton indicated a beta-anomeric configuration (8 LPS. We extracted LPS by the method of Darveau and Hancock (11), which was found to give high yields of both the smooth and rough types of LPS.…”

mentioning

confidence: 99%

Characterization of polysaccharides of Rhizobium meliloti exo mutants that form ineffective nodules

1988

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Mutants of Rhizobium meliloti SU47 with defects in the production of the Calcofluor-binding exopolysaccharide succinoglycan failed to gain entry into alfalfa root nodules. In order to define better the polysaccharide phenotypes of these exo mutants, we analyzed the periplasmic oligosaccharide cyclic (1-2)-p-D-glucan and lipopolysaccharide (LPS) in representative mutants. The exoC mutant lacked the glucan and had abnormal LPS which appeared to lack a substantial portion of the 0 side chain. The exoB mutant had a spectrum of LPS species which differed from those of both the wild-type parental strain and the exoC mutant. The presence of the glucan and normal LPS in the exoA, exoD, exoF, and exoH mutants eliminated defects in these carbohydrates as explanations for the nodule entry defects of these mutants. We also assayed for high-and low-molecular-weight succinoglycans. All of the exo mutants except exoD and exoH completely lacked both forms. For the Calcofluor-dim exoD mutant, the distribution of high-and low-molecular-weight forms depended on the growth medium. The haloless exoH mutant produced high-molecular-weight and only a trace of low-molecular-weight succinoglycan; the succinyl modification was missing, as was expected from the results of previous studies. The implications of these observations with regard to nodule entry are discussed.

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“…Those are complex spectra due to the nature of the studied material. The main signals can be assigned to the saccharide matrix carbons (Fernandez, Reguera, & Ortiz, 2001) but the broad one at 173.53 ppm is mainly due to the carbonyl groups of uronic acids (Casu, 1985). When this signal is decomposed (see Fig.…”

Section: Acidic Character Of the Corn Hull And Its Capacity For Ca Rementioning

confidence: 98%