Nuclear magnetic resonance spectroscopy in solving the analytical problems of medicine: Analysis of cerebrospinal fluid

Kolokolova, T. N.; Savel’ev, O. Yu.; Sergeev, N. M.; Шпигун, О. А.; Sokolov, Konstantin V.; Skvortsova, Veronica I.

doi:10.1134/s106193481010014x

Cited by 4 publications

(1 citation statement)

References 17 publications

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“…For example, increased levels of oxalic and glycolic acids have been measured in CSF of patients with ethylene glycol poisoning or primary hyperoxaluria type 1 [3]. Increased concentrations of the 2and 3-hydroxybutyric, citric, and lactic acids were determined in the CSF of patients with ischemic stroke [12]. Increased concentrations of the 2and 3-hydroxybutyric, citric, and lactic acids were determined in the CSF of patients with ischemic stroke [12].…”

Section: Introductionmentioning

confidence: 99%

Determination of metabolic organic acids in cerebrospinal fluid by microchip electrophoresis

et al. 2014

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A new MCE method for the determination of oxalic, citric, glycolic, lactic, and 2- and 3-hydroxybutyric acids, indicators of some metabolic and neurological diseases, in cerebrospinal fluid (CSF) was developed. MCE separations were performed on a PMMA microchip with coupled channels at lower pH (5.5) to prevent proteins interference. A double charged counter-ion, BIS-TRIS propane, was very effective in resolving the studied organic acids. The limits of detection (S/N = 3) ranging from 0.1 to 1.6 μM were obtained with the aid of contact conductivity detector implemented directly on the microchip. RSDs for migration time and peak area of organic acids in artificial and CSF samples were <0.8 and <9.7%, respectively. Recoveries of organic acids in untreated CSF samples on the microchip varied from 91 to 104%. Elimination of chloride interference, a major anionic constituent of CSF, has been reached by two approaches: (i) the use of coupled channels microchip in a column switching mode when approximately 97-99% of chloride was removed electrophoretically in the first separation channel and (ii) the implementation of micro-SPE with silver-form resin prior to the MCE analysis, which selectively removed chloride from undeproteinized CSF samples.

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Section: Introductionmentioning

confidence: 99%

Determination of metabolic organic acids in cerebrospinal fluid by microchip electrophoresis

et al. 2014

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NMR metabolite quantification of a synthetic urine sample: an inter-laboratory comparison of processing workflows

et al. 2023

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Introduction Absolute quantification of individual metabolites in complex biological samples is crucial in targeted metabolomic profiling. Objectives An inter-laboratory test was performed to evaluate the impact of the NMR software, peak-area determination method (integration vs. deconvolution) and operator on quantification trueness and precision. Methods A synthetic urine containing 32 compounds was prepared. One site prepared the urine and calibration samples, and performed NMR acquisition. NMR spectra were acquired with two pulse sequences including water suppression used in routine analyses. The pre-processed spectra were sent to the other sites where each operator quantified the metabolites using internal referencing or external calibration, and his/her favourite in-house, open-access or commercial NMR tool. Results For 1D NMR measurements with solvent presaturation during the recovery delay (zgpr), 20 metabolites were successfully quantified by all processing strategies. Some metabolites could not be quantified by some methods. For internal referencing with TSP, only one half of the metabolites were quantified with a trueness below 5%. With peak integration and external calibration, about 90% of the metabolites were quantified with a trueness below 5%. The NMRProcFlow integration module allowed the quantification of several additional metabolites. The number of quantified metabolites and quantification trueness improved for some metabolites with deconvolution tools. Trueness and precision were not significantly different between zgpr- and NOESYpr-based spectra for about 70% of the variables. Conclusion External calibration performed better than TSP internal referencing. Inter-laboratory tests are useful when choosing to better rationalize the choice of quantification tools for NMR-based metabolomic profiling and confirm the value of spectra deconvolution tools.

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NMR and pattern recognition methods in metabolomics: From data acquisition to biomarker discovery: A review

Smolinska

Blanchet

Buydens

et al. 2012

Analytica Chimica Acta

349

307

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Nuclear magnetic resonance spectroscopy in solving the analytical problems of medicine: Analysis of cerebrospinal fluid

Cited by 4 publications

References 17 publications

Determination of metabolic organic acids in cerebrospinal fluid by microchip electrophoresis

Determination of metabolic organic acids in cerebrospinal fluid by microchip electrophoresis

NMR metabolite quantification of a synthetic urine sample: an inter-laboratory comparison of processing workflows

NMR and pattern recognition methods in metabolomics: From data acquisition to biomarker discovery: A review

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