Nuclear Magnetic Resonance Spectroscopic Method for Determination of Penicillamine in Capsules

Nelson, James J

doi:10.1093/jaoac/64.5.1174

Cited by 2 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Numerous reports are available for the micro-level determination of sulfur-containing drugs and molecules in biological samples and pharmaceutical preparations [20][21][22][23]. The determination methods includes potentiometry [24], NMR-spectrometry [25], colorimetry [20], chromatography [26][27][28], flow injection analysis [29], fluorimetry [30], voltammetry [31] and spectrophotometry [32,33]. The high initial investment, timeconsuming processes, high costs of sample analysis, and heavy instrumentation are major concerns of many reported methods.…”

Section: Introductionmentioning

confidence: 99%

A Simple and Sensitive Inhibitory Kinetic Method for the Carbocisteine Determination

et al. 2021

View full text Add to dashboard Cite

Abstract. A fast, reproducible, and sensitive method is proposed for the kinetic determination of carbocisteine (CCys). The method depends on the inhibitory property of carbocisteine, which reduces the Hg2+ catalyzed substitution rate of cyanide from [Ru(CN)6]4- with N-R-salt (1-Nitroso-2-naphthol-3,6-disulfonic acid disodium salt) via forming a stable complex with Hg2+. Spectrophotometric measurements were carried out by recording the absorbance at 525 nm (λmax of [Ru(CN)5 Nitroso-R-Salt]3- complex) at a fixed time of 10 and 15 min under the optimized reaction conditions with [N-R-salt] = 4.5 × 10-4 M, I = 0.05 M (KNO3), Temp = 45.0 ± 0.2 o C, pH = 7.0 ± 0.03, [Hg2+] = 8.0 × 10-5 M and [Ru(CN)64-] = 4.25 × 10-5 M. With the proposed method, CCys can be determined quantitatively down to 3.0 × 10-6 M. This methodology can be effectively used for the rapid quantitative estimation of CCys in the pharmaceutical samples with good accuracy and reproducibility. The addition of common excipients in pharmaceuticals even up to 1000 times with [CCys] does not interfere significantly in the estimation of CCys. Resumen. Se propone un método rápido, reproducibley sensible para la determinación cinética de la carbocisteina (CCys). El método depende de la propiedad inhibitoria de la carbocisteina que reduce la tasa de sustitución catalizada por Hg2+ del cianuro de [Ru(CN)6]4- con la sal N-R (sal disódica del ácido 1-Nitroso-2-naftol-3,6-disulfónico) mediante la formación de un complejo estable con Hg2+. Las mediciones espectrofotométricas se llevaron a cabo registrando la absorbancia a 525 nm (λmax del complejo [Ru(CN)5 Sal-Nitroso-R]3-) en un tiempo fijo de 10 y 15 min en las condiciones de reacción optimizadas con [sal-NR] = 4.5 × 10-4 M, I = 0.05 M (KNO3), Temp = 45.0 ± 0.2 o C, pH = 7.0 ± 0.03, [Hg2+] = 8.0 × 10-5 M y [Ru(CN)64-] = 4.25 × 10-5 M. Con el método propuesto, CCys se puede determinar cuantitativamente hasta 3,0 × 10-6 M. Esta metodología se puede utilizar eficazmente para la estimación cuantitativa rápida de CCys en las muestras farmacéuticas con buena precisión y reproducibilidad. La adición de excipientes comunes en productos farmacéuticos incluso hasta 1000 veces con [CCys] no interfiere significativamente en la estimación de CCys.

show abstract

Section: Introductionmentioning

confidence: 99%

A Simple and Sensitive Inhibitory Kinetic Method for the Carbocisteine Determination

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Several methods have been reported for the analysis of D-Penicillamine in both pharmaceutical preparations and biological samples. These methods include colorimetry [3][4][5][6][7][8][9][10], fluorimetry [11], chromatography [12][13][14][15][16][17][18][19], flow injection analysis [20][21][22], electrophoresis [23][24][25], potentiometry [26], voltammetry [27][28][29] and NMR spectrometry [30]. Due to the lack of chromophore and or auxochrome in D-penicillamine molecule, direct spectrophotometry cannot be used for its analysis.…”

Section: Introductionmentioning

confidence: 99%

A New Spectrophotometric Method for Determination of Penicillamine in Pharmaceutical Formulation Using 1, 2-naphthoquine-4-sulfonate (NQS)

Elbashir¹,

Awad²

2013

J Pharmacovigilance

View full text Add to dashboard Cite

A rapid, simple and sensitive method for the determination of D-Penicillamine using sodium 1,2-naphthoquine-4-sulfonate (NQS) has been developed. The method is based on the fact that a brown product can be formed by the reaction between D-Penicillamine and sodium NQS in a buffer solution of a pH 12.0. Beer's law is obeyed in the range 10-30 µg/mL of D-Penicillamine at maximum wavelength of 425 nm. The linear regression equation of the calibration curve is A=0.1684+0.01624C (µg/mL), with a linear regression correlation coefficient of 0.997. The detection limit is 3.12 µg/mL and the recovery rate is in the range of 99.21-108.2%. The method has been successfully applied to the determination of D-Penicillamine in pharmaceutical formulation.

show abstract

Nuclear Magnetic Resonance Spectroscopic Method for Determination of Penicillamine in Capsules

Cited by 2 publications

References 0 publications

A Simple and Sensitive Inhibitory Kinetic Method for the Carbocisteine Determination

A Simple and Sensitive Inhibitory Kinetic Method for the Carbocisteine Determination

A New Spectrophotometric Method for Determination of Penicillamine in Pharmaceutical Formulation Using 1, 2-naphthoquine-4-sulfonate (NQS)

Contact Info

Product

Resources

About