Nuclear magnetic resonance micro-imaging and X-radiography as possible techniques to study seed germination

Foucat, Loı̈c; Chavagnat, A.; Renou, Jean-Pierre

doi:10.1016/0304-4238(93)90043-p

Cited by 22 publications

(10 citation statements)

References 6 publications

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“…The interest in Xray test has been growing in seed technology because it analyses quickly internal seed morphology and provides the detection of possible embryo damage and abnormalities without seed destruction. In addition to these analysis characteristics, the X-ray test may be applied to the study of physiological changes during maturation, germination and conditioning processes (Simak, 1991;Foucat et al, 1993;Dell'Aquila, 2007a) and in breeding research (Bino et al, 1993). The viability of seed submitted to X-ray is not affected because non lethal radiation doses are generally used during the test (Bino et al, 1993;ISTA, 1995).…”

Section: Introductionmentioning

confidence: 99%

An assessment of mechanical and stink bug damage in soybean seed using X-ray analysis test

Pinto

Cícero²,

França-Neto³

et al. 2009

Seed Sci. Technol.

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The X-ray image analysis technique is a precise method that enables examination of regions that are damaged and/or altered in seed and their location and extension. It is a non-destructive method allowing the X-ray treated seed to be submitted to quality physiological tests. The objective of this study was to use X-ray image analysis technique to identify mechanical and stink bug damages in soybean seed. Seeds from different batches of the BRS184 cultivar were sorted by X-ray test and then were tested for germination with the aim to relate the damage with possible seed viability loss. Alternatively, the tetrazolium test was carried out to be compared with the X-ray test. Damage severity and location were considered for the interpretive analysis of X-ray images. The results showed that the X-ray image analysis technique was effective in detecting mechanical and stink bug damage in soybean seed.

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Section: Introductionmentioning

confidence: 99%

An assessment of mechanical and stink bug damage in soybean seed using X-ray analysis test

Pinto

Cícero²,

França-Neto³

et al. 2009

Seed Sci. Technol.

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“…Nuclear magnetic resonance (NMR) micro-imaging combined with X-radiography have been applied to the study of pepper seed internal morphology in quiescent and imbibing status (Foucat et al 1993). NMR spectroscopy has been also used in comparing spinspin relaxation time with germination of soybean and wheat (Krishnan et al 2004).…”

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confidence: 99%

Pepper seed germination assessed by combined X-radiography and computer-aided imaging analysis

Dell’Aquila¹

2007

Biol. plant.

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A lot of pepper seeds having 87 % germination were subjected to X-ray inspection using a non lethal dose of radiation. Seeds with less than 2.7 % (on the basis of total seed area) of free space area, i.e. the spaces between embryo and endosperm, were classified as highly viable seeds (97 -100 % germination) with the lowest level of abnormal seedlings. Seeds X-ray classified as good were subjected to a computerised image analysis to study seed imbibition and radicle elongation. The patterns of seed area increase, chosen as the most accurate indicator of seed swelling, resembled the triphasic curve of water uptake. The first phase was completed at 9 h followed by a second phase that varied widely in time until completion of germination between 52 and 96 h. The proportion of seeds with radicle protrusion between 52 -56 h and 64 -72 h assessed with the image analysis was significantly higher than that recorded using a conventional germination test. In addition, the rate of increase of seed area during the third phase of imbibition, mostly due to protrusion of the radicle tip and its growth, was highly correlated with the corresponding radicle elongation rate.Additional key words: Capsicum annuum L., internal seed morphology, seed swelling.

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“…Automatic acquisition was performed of a series of X-ray tomographic reconstructions over time on the seed. With X-ray tomography, only the contrast between air and tissues is present [19]. During the imbibition process, the volume of air is reduced and progressively shrinks to zero.…”

Section: Acquisition Protocolmentioning

confidence: 99%

Evaluation of 3D/2D Imaging and Image Processing Techniques for the Monitoring of Seed Imbibition

et al. 2018

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Seed imbibition is a very important process in plant biology by which, thanks to a simple water income, a dry seed may turn into a developing organism. In natural conditions, this process occurs in the soil, e.g., with difficult access for a direct observation. Monitoring the seed imbibition with non-invasive imaging techniques is therefore an important and possibly challenging task if one tries to perform it in natural conditions. In this report, we describe a set of four different imaging techniques that enable to addressing this task either in 3D or in 2D. For each technique, the following items are proposed. A detailed experimental protocol is provided to acquire images of the imbibition process. With the illustration of real data, the significance of the physical quantities measured in terms of their relation to the income of water in the seed is presented. Complete image analysis pipelines are then proposed to extract dynamic information on the imbibition process from such monitoring experiments. A final discussion compares the advantages and current limitations of each technique in addition to elements concerning the associated throughput and cost. These are criteria especially relevant in the field of plant phenotyping where large populations of plants are imaged to produce quantitatively significative traits after image processing.

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Nuclear magnetic resonance micro-imaging and X-radiography as possible techniques to study seed germination

Cited by 22 publications

References 6 publications

An assessment of mechanical and stink bug damage in soybean seed using X-ray analysis test

An assessment of mechanical and stink bug damage in soybean seed using X-ray analysis test

Pepper seed germination assessed by combined X-radiography and computer-aided imaging analysis

Evaluation of 3D/2D Imaging and Image Processing Techniques for the Monitoring of Seed Imbibition

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