Nuclear magnetic resonance metabolomic profiling of urine provides a noninvasive alternative to the identification of biomarkers associated with endometriosis

Vicente-Muñoz, Sara; Morcillo, Inmaculada; Puchades‐Carrasco, Leonor; Payá, Vicente; Pellicer, António; Pineda‐Lucena, Antonio

doi:10.1016/j.fertnstert.2015.07.1149

Cited by 55 publications

(35 citation statements)

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“…Increased levels of uracil, or other uracil-containing metabolites (e.g., 2′-deoxyuridine) (Mondul et al 2015), have been found in previous PCa studies (Jiang et al 2010; McDunn et al 2013; Spur et al 2013; Sreekumar et al 2009) suggesting an important role of the metabolism of this compound in this disease. Alterations in the levels of pseudouridine have also been observed in other pathological processes (Rasmuson and Bjork 1995; Vicente-Munoz et al 2015; Masaki et al 2006) and have been associated with disease activity, tumor burden, and clinical status (Tamura et al 1987). …”

Section: Discussionmentioning

confidence: 94%

Non-invasive urinary metabolomic profiling discriminates prostate cancer from benign prostatic hyperplasia

et al. 2017

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IntroductionProstate cancer (PCa) is one of the most common malignancies in men worldwide. Serum prostate specific antigen (PSA) level has been extensively used as a biomarker to detect PCa. However, PSA is not cancer-specific and various non-malignant conditions, including benign prostatic hyperplasia (BPH), can cause a rise in PSA blood levels, thus leading to many false positive results.ObjectivesIn this study, we evaluated the potential of urinary metabolomic profiling for discriminating PCa from BPH.MethodsUrine samples from 64 PCa patients and 51 individuals diagnosed with BPH were analysed using 1H nuclear magnetic resonance (1H-NMR). Comparative analysis of urinary metabolomic profiles was carried out using multivariate and univariate statistical approaches.ResultsThe urine metabolomic profile of PCa patients is characterised by increased concentrations of branched-chain amino acids (BCAA), glutamate and pseudouridine, and decreased concentrations of glycine, dimethylglycine, fumarate and 4-imidazole-acetate compared with individuals diagnosed with BPH.ConclusionPCa patients have a specific urinary metabolomic profile. The results of our study underscore the clinical potential of metabolomic profiling to uncover metabolic changes that could be useful to discriminate PCa from BPH in a clinical context.

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Section: Discussionmentioning

confidence: 94%

Non-invasive urinary metabolomic profiling discriminates prostate cancer from benign prostatic hyperplasia

et al. 2017

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“…Metabolomics analysis in endometriosis has been performed in peripheral blood, peritoneal fluid, follicular fluid and urine [25][26][27][28]. According to the widely accepted theory of retrograde menstruation, the endometrium is the source of ectopic endometriotic foci.…”

Section: Discussionmentioning

confidence: 99%

Endometrium metabolomic profiling reveals potential biomarkers for diagnosis of endometriosis at minimal-mild stages

Liang

2018

Fertility and Sterility

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Background: The sensitivity and specificity of non-invasive diagnostic methods for endometriosis, especially at early stages, are not optimal. The clinical diagnostic indicator cancer antigen 125 (CA125) performs poorly in the diagnosis of minimal endometriosis, with a sensitivity of 24%. Therefore, it is urgent to explore novel diagnostic biomarkers. We evaluated the metabolomic profile variation of the eutopic endometrium between minimal-mild endometriosis patients and healthy women by ultra-high-performance liquid chromatography coupled with electrospray ionization high-resolution mass spectrometry (UHPLC-ESI-HRMS). Methods: Our study comprised 29 patients with laparoscopically confirmed endometriosis at stages I-II and 37 infertile women who underwent diagnostic laparoscopy combined with hysteroscopy from January 2014 to January 2015. Eutopic endometrium samples were collected by pipelle endometrial biopsy. The metabolites were quantified by UHPLC-ESI-HRMS. The best combination of biomarkers was then selected by performing step-wise logistic regression analysis with backward elimination. Results: Twelve metabolites were identified as endometriosis-associated biomarkers. The eutopic endometrium metabolomic profile of the endometriosis patients was characterized by a significant increase in the concentration of hypoxanthine, L-arginine, L-tyrosine, leucine, lysine, inosine, omega-3 arachidonic acid, guanosine, xanthosine, lysophosphatidylethanolamine and asparagine. In contrast, the concentration of uric acid was decreased. Metabolites were filtered by step-wise logistic regression with backward elimination, and a model containing uric acid, hypoxanthine, and lysophosphatidylethanolamine was constructed. Receiver-operating characteristic (ROC) analysis confirmed the prognostic value of these parameters for the diagnosis of minimal/mild endometriosis with a sensitivity of 66.7% and a specificity of 90.0%. Conclusions: Metabolomics analysis of the eutopic endometrium in endometriosis was effectively characterized by UHPLC-ESI-HRMS-based metabolomics. Our study supports the importance of purine and amino acid metabolites in the pathophysiology of endometriosis and provides potential biomarkers for semi-invasive diagnosis of early-stage endometriosis.

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“…Adrenal gland is the endocrine gland that plays an important role in the process of stress reactio, the hormones secreted by adrenal medulla and cortex are closely related to the activation of stress reaction [12]. Adrenal medullary secretion function is associated with the excitability of sympathetic nervous system, and the stress reaction caused by trauma could increase the excitability of sympathetic nervous system, which causes the massive release of NE and E and leads to vasoconstriction and myocardial contractility enhancement [13][14]; the adrenocortical secretion function is regulated by HPA axis, hypothalamus signal peptide acts on pituitary gland and stimulate the synthesis and release of ACTH, ACTH acts on adrenal cortex and causes massively secretion of Cor, and the Cor can on the one hand, enhance the ability of the body to endure trauma [15], and on the other hand promote glycogen decomposition and elevate blood sugar levels, and also stimulate the compensatory secretion of insulin [16][17]. In the study, analysis of the contents of adrenal gland-related endocrine hormones showed that serum NE, E, ACTH, Cor and INS levels of laparoscopic group 1d and 3d after surgery were significantly lower than those of laparotomy group.…”

Section: Discussionmentioning

confidence: 99%

Comparison between laparoscopic surgery and laparotomy for the treatment of acute ruptured ectopic pregnancy

Zhou

2017

JAD

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Nuclear magnetic resonance metabolomic profiling of urine provides a noninvasive alternative to the identification of biomarkers associated with endometriosis

Cited by 55 publications

References 49 publications

Non-invasive urinary metabolomic profiling discriminates prostate cancer from benign prostatic hyperplasia

Non-invasive urinary metabolomic profiling discriminates prostate cancer from benign prostatic hyperplasia

Endometrium metabolomic profiling reveals potential biomarkers for diagnosis of endometriosis at minimal-mild stages

Comparison between laparoscopic surgery and laparotomy for the treatment of acute ruptured ectopic pregnancy

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