Nuclear magnetic resonance-based metabolomic analysis in the assessment of preclinical atherosclerosis in type 1 diabetes and preeclampsia

Amor, Antonio J.; Vinagre, Irene; Valverde, Maite; Urquizu, Xavier; Meler, E.; López, Edith; Alonso, Núria; Pané, Adriana; Giménez, Marga; Codina, Laura; Conget, Ignacio; Barahona, María José; Perea, Verónica

doi:10.1016/j.diabres.2020.108548

Cited by 6 publications

(9 citation statements)

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“…Importantly, glycemic optimization produced a favorable decrease in IDL-C and IDL-TG and thus atherogenic remnants, which in turn improved lipoprotein atherogenicity indexes in controlled T1D subjects. Because the lipoprotein pro le of T1D subjects used in our previous study were analyzed in subjects with a reasonable diabetic control [11], IDL could plausibly be within the normal range de ned by the non-diabetic subjects and thus not detected even in the case that serum IDL-C could have been eventually measured. Of note, these data highlight the importance of measuring serum IDL-C and IDL-TG to further characterize the atherogenic lipoprotein pro le in subjects with T1D and thus their contribution to elevated CVD risk.…”

Section: Discussionmentioning

confidence: 99%

“…An altered, proatherogenic lipoprotein pro le is often obscured by apparently normal serum concentrations of total triglycerides (TG), total cholesterol, low-density lipoprotein and high-density lipoprotein cholesterol (LDL-C and HDL-C, respectively) in subjects with poorly controlled T1D [2,[9][10][11].…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, normal LDL-C values may coexist with increased intermediate-density lipoproteins (IDL), and small and dense LDL (sdLDL) in T1D subjects [12,13]. Likewise, HDL-C values may be normal or even moderately elevated in T1D subjects [11,14,15], but HDL in diabetes may display poorer antiatherogenic properties [16,17], due at least in part to the cumulative associated glycoxidative modi cations of HDL proteins [14].…”

Section: Introductionmentioning

confidence: 99%

“…Advanced lipoprotein analysis using nuclear magnetic resonance (1H-NMR) spectroscopy allows the screening and characterization of circulating lipoprotein classes [11,18], and the identi cation of speci c lipoprotein characteristics, such as size and composition, provided by this advanced analysis may be potentially useful in timely and accurately predicting CVD risk in subjects with T1D.…”

Section: Introductionmentioning

confidence: 99%

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Outstanding Improvement of the Advanced Lipoprotein Profile in Subjects with New-Onset Type 1 Diabetes Mellitus After Achieving Optimal Glycemic Control

Castelblanco

Hernández

Ortega

et al. 2021

Preprint

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Background: An altered lipoprotein profile is often hidden in normolipidemic subjects with uncontrolled type 1 diabetes mellitus (T1D). Conceivably, its amelioration after glycemic control may be overlooked in controlled T1D subjects. In this regard, we investigated the influence of glycemic optimization on lipoprotein subfraction parameters in normolipidemic new-onset T1D subjects.Methods: Twelve subjects at onset of T1D (5 women and 7 men) were studied. Serum lipid and advanced lipoprotein profiles were determined by routine laboratory methods and 1H-NMR spectroscopy shortly after diabetes diagnosis (baseline), and after achieving optimal glycemic control (HbA1c<7%) at a follow-up of 11.3 to 25.6 weeks.Results: Advanced lipoprotein analysis revealed a significant reduction from baseline in plasma concentrations of triglycerides (TG) and cholesterol (C), and in apolipoprotein (Apo)B-containing lipoproteins of treated subjects (VLDL-TG: -21%, LDL-TG: -36%, IDL-TG: -32%, LDL-TG: -36%, P<0.05; VLDL-C: -46%, IDL-C: -46%, LDL-C: -16%; P<0.05). Additionally, neither VLDL-TG and LDL-TG nor LDL-C of T1D subjects at follow-up differed from those of non-diabetic subjects. Decreased VLDL and LDL lipids were mainly attributed to concomitant reductions in the concentration of medium-sized VLDL (-39%) and medium-sized LDL (34%) and, to a lesser extent, to large-sized LDL (13%), respectively. Notably, proatherogenic IDL characteristics and related surrogates of atherogenicity were resolved upon intensive glycemic control. However, neither the concentration of HDL-P, nor the relative proportion of HDL sizes was influenced by the glucose-lowering therapy.Conclusions: In otherwise normolipidemic T1D subjects according to the conventional lipid profile, the achievement of optimal glycemic control after diabetes onset is associated with normalization of profound derangements in ApoB-containing lipoproteins (VLDL, IDL, and LDL). Whether higher cardiovascular risk in poorly controlled T1D is partially mediated by these proatherogenic lipid alterations should be elucidated by further studies.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Outstanding Improvement of the Advanced Lipoprotein Profile in Subjects with New-Onset Type 1 Diabetes Mellitus After Achieving Optimal Glycemic Control

Castelblanco

Hernández

Ortega

et al. 2021

Preprint

View full text Add to dashboard Cite

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“…Atherosclerosis (AS) is a leading cause of coronary heart disease. [1][2][3][4] Vascular endothelial cells (VECs) are a major cell type that reside in the arterial walls, and aberrant behaviors of VECs may contribute to the development of AS. 5 A previous study found that excessive reactive oxygen species (ROS) produced as a result of external stimulation leads to oxidative stress injury, which is the main cause of endothelial dysfunction.…”

Section: Introductionmentioning

confidence: 99%

Effect of Ginsenoside Rh1 on Proliferation, Apoptosis, and Oxidative Stress in Vascular Endothelial Cells by Regulation of the Nuclear Erythroid 2-related Factor-2/Heme Oxygenase-1 Signaling Pathway

Jiang

et al. 2022

Journal of Cardiovascular Pharmacology

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:This study aimed to investigate the role of ginsenoside Rh1 in regulating the proliferation, apoptosis, and oxidative stress in oxidized low-density lipoprotein (ox-LDL)-treated human vascular endothelial cells (VECs) and the underlying mechanisms. VECs were treated with ox-LDL to generate an in vitro atherosclerosis model. The effect of ginsenoside Rh1 on cell viability and proliferation was examined by MTT and colony formation assays, respectively, and cell apoptosis was determined by flow cytometry and transferase dUTP nick end-labeling assay. The levels of reactive oxygen species, malondialdehyde, and superoxide dismutase activity were detected using biological assays. Finally, the effect of ginsenoside Rh1 on the levels of BAX and BCL-2 and the nuclear erythroid 2-related factor-2/heme oxygenase (HO)-1 signaling pathway was determined by quantitative real-time polymerase chain reaction and western blot assays. Treatment with ginsenoside Rh1 significantly increased the proliferation and decreased the apoptosis of ox-LDL–treated VECs in a dose-dependent manner. Moreover, ginsenoside Rh1 also relieved oxidative stress in ox-LDL–treated VECs by activating the Nrf2/HO-1 signaling pathway. Thus, ginsenoside Rh1 affects the proliferation, apoptosis, and oxidative stress in ox-LDL–treated VECs by activating the Nrf2/HO-1 signaling pathway.

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Lipidomic signatures in patients with early-onset and late-onset Preeclampsia

Huang,

Sun,

Zhou

et al. 2024

Metabolomics

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Background Preeclampsia is a pregnancy-specific clinical syndrome and can be subdivided into early-onset preeclampsia (EOPE) and late-onset preeclampsia (LOPE) according to the gestational age of delivery. Patients with preeclampsia have aberrant lipid metabolism. This study aims to compare serum lipid profiles of normal pregnant women with EOPE or LOPE and screening potential biomarkers to diagnose EOPE or LOPE. Methods Twenty normal pregnant controls (NC), 19 EOPE, and 19 LOPE were recruited in this study. Untargeted lipidomics based on ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was used to compare their serum lipid profiles. Results The lipid metabolism profiles significantly differ among the NC, EOPE, and LOPE. Compared to the NC, there were 256 and 275 distinct lipids in the EOPE and LOPE, respectively. Furthermore, there were 42 different lipids between the LOPE and EOPE, of which eight were significantly associated with fetal birth weight and maternal urine protein. The five lipids that both differed in the EOPE and LOPE were DGTS (16:3/16:3), LPC (20:3), LPC (22:6), LPE (22:6), PC (18:5e/4:0), and a combination of them were a potential biomarker for predicting EOPE or LOPE. The receiver operating characteristic analysis revealed that the diagnostic power of the combination for distinguishing the EOPE from the NC and for distinguishing the LOPE from the NC can reach 1.000 and 0.992, respectively. The association between the lipid modules and clinical characteristics of EOPE and LOPE was investigated by the weighted gene co-expression network analysis (WGCNA). The results demonstrated that the main different metabolism pathway between the EOPE and LOPE was enriched in glycerophospholipid metabolism. Conclusions Lipid metabolism disorders may be a potential mechanism of the pathogenesis of preeclampsia. Lipid metabolites have the potential to serve as biomarkers in patients with EOPE or LOPE. Furthermore, lipid metabolites correlate with clinical severity indicators for patients with EOPE and LOPE, including fetal birth weight and maternal urine protein levels.

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Nuclear magnetic resonance-based metabolomic analysis in the assessment of preclinical atherosclerosis in type 1 diabetes and preeclampsia

Cited by 6 publications

References 53 publications

Outstanding Improvement of the Advanced Lipoprotein Profile in Subjects with New-Onset Type 1 Diabetes Mellitus After Achieving Optimal Glycemic Control

Outstanding Improvement of the Advanced Lipoprotein Profile in Subjects with New-Onset Type 1 Diabetes Mellitus After Achieving Optimal Glycemic Control

Effect of Ginsenoside Rh1 on Proliferation, Apoptosis, and Oxidative Stress in Vascular Endothelial Cells by Regulation of the Nuclear Erythroid 2-related Factor-2/Heme Oxygenase-1 Signaling Pathway

Lipidomic signatures in patients with early-onset and late-onset Preeclampsia

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