Nuclear magnetic resonance and x-ray studies on micellar aggregates of sodium deoxycholate

Conte, Gennaro; Blasi, R. Di; Giglio, E.; Parretta, A.; Pavel, Nicolae Viorel

doi:10.1021/j150667a052

Cited by 104 publications

(86 citation statements)

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“…The best results were achieved by using 0.06 M NaCh, which is in agreement with a previous study (Ji et al, 2013). This could be due to the fact that such a concentration is nearly above the secondary CMC (0.05 M) reported for sodium cholate at 25 C (O'Connor et al, 1983;Conte et al, 1984;Santhanalakshmi et al, 2001), which favors the 123 I-Hyp/Hyp solubilization. At surfactant concentration above the CMC, the formed micelles are able to accommodate the hydrophobic molecule due to an increase in micelle-bound solutes, thus improving the solubility of the molecule in the medium.…”

Section: Discussionsupporting

confidence: 89%

“…At a certain concentration (0.013-0.018 M) known as critical micellar concentration (CMC), it forms micelles that act as an encapsulating agent for dietary fats, hydrophobic substances and guarantee efficient transport and absorption of poorly water soluble nutrients. Due to the enlargement of the size of the primary micelles caused by the hydrogen bonding forces among the hydrophilic regions, a secondary micellization occurs at higher concentration of NaCh (0.05 M) (O'Connor et al, 1983;Conte et al, 1984;Santhanalakshmi et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

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Sodium cholate, a solubilizing agent for the necrosis avid radioiodinated hypericin in rabbits with acute myocardial infarction

et al. 2014

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(2015) Sodium cholate, a solubilizing agent for the necrosis avid radioiodinated hypericin in rabbits with acute myocardial infarction, Drug Delivery, 22:3, 427-435, DOI: 10.3109/10717544.2013 I-Hyp) and investigated biodistribution and targetability of this formulation in rabbits with acute myocardial infarction (AMI). Materials and methods: Solubility of radioiodinated hypericin/hypericin (Hyp) in NaCh solutions was evaluated by microscopy. Hyp with 123 I-sodium iodide was performed using hydrogen peroxide as oxidant in 0.06 M NaCh. Radiochemical yield determination and purification were conducted using high performance liquid chromatography.123 I-Hyp was solubilized in 0.06 M NaCh containing 1.9 Â 10 À4 M Hyp. The formulation was macroscopically inspected and intravenously injected to five rabbits with AMI. At 24 h, biodistribution was evaluated by tissue gamma counting (TGC) and necrosis targetability was assessed by TGC, autoradiography, fluorescence examination and histology. Results: Microscopically NaCh at 0.06 M shows the best properties for solubilizing the radioiodinated Hyp/Hyp. 123I-Hyp in 0.06 M NaCh was achieved in 85% with radiochemical purity of 99% after purification. NaCh-dissolved 123 I-Hyp/Hyp shows no particles. By TGC, animals exhibited higher (p ¼ 0.003) radioactivity accumulation in AMI (0.8 ± 0.2% ID/g) than in normal myocardium (0.05 ± 0.02% ID/g), as confirmed by autoradiography, fluorescence measurement and histology. Among organs, the highest uptake of radioactivity was found in liver (15.7 ± 0.6% ID), large (9.7 ± 1.0% ID) and small (5.9 ± 0.6% ID) intestines. Conclusion: Necrosis avidity of NaCh-dissolved 123 I-Hyp/Hyp and its hepatobiliary excretion were demonstrated. The suitability of NaCh as solubilizing agent of 123 I-Hyp for hotspot imaging of AMI was proved.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Sodium cholate, a solubilizing agent for the necrosis avid radioiodinated hypericin in rabbits with acute myocardial infarction

et al. 2014

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“…26 When pH was varied, there was no precipitation below pH 6.8, but there was formation of Ca(HB) 2 at pH 7.1 fibers (elongated aggregates) of the Na / and Rb / salts of various bile acids. [41][42][43][44][45][46] The results, supported by potential and of CaB at pH 8.2 or higher. These findings were compatible with successive ionization of the two -COOH groups be-energy calculations, point to elongated helical structures for the bile salt aggregates, with the nonpolar groups at the petween pH 7.0 and 8.5.…”

Section: Structure Of Calcium and Copper Salts Of Bilirubinmentioning

confidence: 83%

New concepts in bilirubin and jaundice: Report of the Third International Bilirubin Workshop, April 6-8, 1995, Trieste, Italy

Tiribelli

Ostrow

1996

Hepatology

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only partial and temporary relief of hyperbilirubinemia; tocytic transporters of UCB and related organic anions extracorporeal liver assist devices have had some sucwere described. Special emphasis was given to the adencess in initial human studies; and inhibition of heme osine triphosphate (ATP)-dependent canalicular multioxygenase (HO) with metalloporphyrins, especially tin specific organic anion transporter that is defective in mesoporphyrin, which markedly decreases bilirubin production for prolonged periods, is a new alternative dipyrrole (Fig. 1). In solution, however, this structure is flexi- Received October 3, 1995; accepted June 6, 1996. ble, and small changes ({40Њ) in the interplanar angle (u)

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“…Na-DOC is a steroid with C-24 carboxylate group ( Figure 3a) and joines to a polar head group and like bile salts and forms small micelles. Micelle growth in bile saltsis documented by a lot of experimental evidence, based on viscosity and hydrogen bonding was noted as the driving force in the micellar aggregation process [15,16]. Such data pointed out the existence of a continuous process driven by the electrostatic repulsions [17].…”

Section: Resultsmentioning

confidence: 93%

Gelation of Sodium-Deoxycholate: Influenced by the Presence of a CorticosteroidBetamethasone-17-Valerate

Homan¹

2017

J. Drug

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This study was designed to evaluate the possible influences of betamethasone-17-valerate (BMV) on the gelation properties of sodium deoxycholate (Na-DOC), due to structural similarities as the monomer and the active agent. For this purpose gel formulations at two different concentrations, 0.05 and 0.1 % (w/w) were prepared and characterized by rheological and thermal analysis. The results indicated that the elastic moduli of the gels increased with increasing concentration of BMV in the system. This phenomenon was shown to be due to the modification of polymerization occurred by interactions between BMV and Na-DOC. The increment of BMV concentration in the formulation, caused a modification of the glass transition temperature (Tg) detected. Tg data pointed out a higher polymerization of Na-DOC with increasing amount of BMV. Transepidermal water loss measurements were also performed to evaluate possible skin irritation. It was observed that the degree of Na-DOC polymerization did not show any irritant effect on the skin.Overall, the results of this study clearly confirmed that incorporation of BMV into Na-DOC gel system produced a synergistic effect on product rheological characteristics, which would be beneficial for topical applications.

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Nuclear magnetic resonance and x-ray studies on micellar aggregates of sodium deoxycholate

Cited by 104 publications

References 4 publications

Sodium cholate, a solubilizing agent for the necrosis avid radioiodinated hypericin in rabbits with acute myocardial infarction

Sodium cholate, a solubilizing agent for the necrosis avid radioiodinated hypericin in rabbits with acute myocardial infarction

New concepts in bilirubin and jaundice: Report of the Third International Bilirubin Workshop, April 6-8, 1995, Trieste, Italy

Gelation of Sodium-Deoxycholate: Influenced by the Presence of a CorticosteroidBetamethasone-17-Valerate

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