Nuclear localization of vasoactive intestinal peptide (VIP) receptors in human breast cancer

Valdehíta, Ana; Bajo, Ana M.; Fernández‐Martínez, Ana B.; Arenas, María Isabel; Vacas, Eva; Valenzuela, Pedro L.; Ruíz-Villaespesa, Antonio; Prieto, Juan Carlos; Carmena, Marı́a J.

doi:10.1016/j.peptides.2010.07.024

Cited by 52 publications

(43 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some studies have shown nuclear and perinuclear localization of G proteincoupled receptors. 116,117 One specific group reported the localization of a NPY receptor in the nucleus membrane. However, in their studies the NPY Y 1 receptor was localized in the nuclear envelope of 20-week-old human fetal endocardial endothelial cells and cells of adult rat hearts.…”

Section: Discussionmentioning

confidence: 99%

Neuropeptide Y Receptors Y₁and Y₂are Present in Neurons and Glial Cells in Rat Retinal Cells in Culture

Santos-Carvalho

Aveleira²,

Elvas

et al. 2013

Invest. Ophthalmol. Vis. Sci.

View full text Add to dashboard Cite

PURPOSE. Neuropeptide Y (NPY) is one of the most abundant peptides in the central nervous system (CNS), including the retina. This peptide activates various different G-coupled receptors (NPY Y 1 , Y 2 , Y 4 , and Y 5 ) that are also present in the retina. However, the localization of NPY receptors in the several types of retinal cells is not completely known. In this study, we have looked at the distribution of NPY Y 1 and Y 2 receptors in rat retinal cells to reveal new perspectives on the role of NPY receptors in retina physiology. METHODS.Rat retinal neural cell cultures were prepared from newborn Wistar rats (P3-P5) and pure rat Müller cell culture was obtained after treatment of these cells with ascorbic acid. 1 This 36-amino acid peptide was first isolated from the pig brain in 1982 by Tatemoto.2 NPY is one of the most abundant peptides in the central nervous system (CNS), including the retina, and is involved in numerous physiologic functions, such as feeding, memory processing, and cognition.3,4 NPY actions are mediated by G protein-coupled receptors, which have been named NPY Y 1 , Y 2 , Y 4 , Y 5 , and y 6 . 3,5 NPY is expressed in the retina of mammals and nonmammals. [6][7][8][9][10][11][12] In rodent retinas, NPY-immunoreactivity (NPY-ir) is present in the inner retina, it is localized in cell bodies at both inner nuclear layer (INL) and ganglion cell layer (GCL), and is also present in processes located in the inner plexiform layer (IPL).11,13 NPY shows one of the highest degrees of phylogenetic preservation, compared with other neuropeptides, and does not show marked differences between species.14,16 From a consideration of the phylogenetic preservation, it is expected that the NPY and NPY receptor distribution in the retina does not vary significantly between species. NPY-ir is present in amacrine cells in the majority of species studied (fish, frogs, lizards, rodents, baboons, pigs, cats, chickens, and pigeons). Cats, dogs, dolphins, and humans also show NPY in their ganglion cells, 9,17-20 while some turtles, lizards, and frogs present NPY-ir in bipolar cells. 8,21,22 Few studies have analyzed the distribution of NPY receptors either in the retina or in specific retinal cell types. The presence of both Y 1 and Y 2 receptors in the mouse retina has been confirmed by mRNA analysis 13,23 ; however, it is unclear which cell types express these receptors. Moreover, in primary cultures of Müller cells isolated from the retina of guinea pig, a functional assay of cell proliferation suggested the presence of NPY Y 1 receptor in these glial cells. 24 In human retinal pigment epithelium (RPE), mRNA encoding for NPY Y 1 , Y 2 , and Y 5 receptors has been detected, while in bovine RPE only mRNAs encoding for NPY Y 1 and Y 2 receptors have been found. 25 Although the presence of NPY receptors in the rat retina has been described, in particular in NPY Y 1 , Y 2 , Y 4 , and Y 5 mRNA expression, 26,27 little is known about their localization. The NPY Y 1 receptor-ir is localized in horizontal and amacrine ce...

show abstract

Section: Discussionmentioning

confidence: 99%

Neuropeptide Y Receptors Y₁and Y₂are Present in Neurons and Glial Cells in Rat Retinal Cells in Culture

Santos-Carvalho

Aveleira²,

Elvas

et al. 2013

Invest. Ophthalmol. Vis. Sci.

View full text Add to dashboard Cite

show abstract

“…Consequently, strategies aiming to target these receptors for tumors imaging or therapy are under consideration [11]. An atypical nuclear localization of VIP receptors has been reported in a human colonic adenocarcinoma cell line [12] and VPAC1 nuclear receptors have been observed in human breast cancer [13] and renal carcinoma [14] cells. It has been hypothesized that nuclear membrane VPAC1/2 receptors could be involved in the proliferation, survival and differentiation of Th2 lymphocytes [15].…”

Section: Introductionmentioning

confidence: 99%

Atypical nuclear localization of VIP receptors in glioma cell lines and patients

Barbarin

Seité

Godet

et al. 2014

Biochemical and Biophysical Research Communications

View full text Add to dashboard Cite

“…Further investigations established the presence of neuropeptide receptors, e.g. VPAC1, VPAC2 and PACAP receptors, respectively, in prostate, breast and lung cancer tissues [24][25][26][27][28]. The occurrence of neuropeptides and their receptors within tumor may strongly suggest an autocrine pattern of signaling resulting in a faster tumor growth.…”

Section: Discussionmentioning

confidence: 99%

Plasticity of neuropeptidergic neoplasm cells in the primary and metastatic Merkel cell carcinoma

Godlewski

Kowalczyk

Kozielec

et al. 2013

Folia Histochem Cytobiol.

View full text Add to dashboard Cite

Merkel cell carcinoma (MCC) is a rare and highly aggressive cutaneous carcinoma with characteristics of neuroendocrine tumor. We performed immunohistochemical analysis to demonstrate the presence of various neuropeptides within cells of MCC resected from a 75-year old woman. The cells of primary tumor of cheek were compared with the cells of regional right submandibular metastatic tumor which was found eight months later. A double-staining IHC for the pan-neuronal marker, PGP 9.5, and selected neuropeptides in the tissue material obtained from both locations was performed. Single multipolar cells in the main mass of primary tumor stained positively for PGP 9.5 and such neuropeptides as GAL, VIP, PACAP, NPY and CGRP. Moreover, we demonstrated for the first time the presence of neuropeptides in metastatic MCC cells. In the metastatic tumor, cells showing the co-localization of PGP-9.5 and neuropeptides were more numerous, mostly of oval shape, and significantly smaller than in the primary tumor. Thus, the progression of MCC may be associated with the acquisition by its cells of new morphological and biological features.

show abstract

Nuclear localization of vasoactive intestinal peptide (VIP) receptors in human breast cancer

Cited by 52 publications

References 53 publications

Neuropeptide Y Receptors Y₁and Y₂are Present in Neurons and Glial Cells in Rat Retinal Cells in Culture

Neuropeptide Y Receptors Y₁and Y₂are Present in Neurons and Glial Cells in Rat Retinal Cells in Culture

Atypical nuclear localization of VIP receptors in glioma cell lines and patients

Plasticity of neuropeptidergic neoplasm cells in the primary and metastatic Merkel cell carcinoma

Contact Info

Product

Resources

About

Nuclear localization of vasoactive intestinal peptide (VIP) receptors in human breast cancer

Cited by 52 publications

References 53 publications

Neuropeptide Y Receptors Y1and Y2are Present in Neurons and Glial Cells in Rat Retinal Cells in Culture

Neuropeptide Y Receptors Y1and Y2are Present in Neurons and Glial Cells in Rat Retinal Cells in Culture

Atypical nuclear localization of VIP receptors in glioma cell lines and patients

Plasticity of neuropeptidergic neoplasm cells in the primary and metastatic Merkel cell carcinoma

Contact Info

Product

Resources

About

Neuropeptide Y Receptors Y₁and Y₂are Present in Neurons and Glial Cells in Rat Retinal Cells in Culture

Neuropeptide Y Receptors Y₁and Y₂are Present in Neurons and Glial Cells in Rat Retinal Cells in Culture