Nuclear localization of Meis1 in dermal papilla promotes hair matrix cell proliferation in the anagen phase of hair cycle

Namekata, Masato; Yamamoto, Masakuni; Goitsuka, Ryo

doi:10.1016/j.bbrc.2019.09.060

Cited by 6 publications

(6 citation statements)

References 24 publications

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“…Among these cells, dermal papilla cells (DPCs), hair follicle stem cells (HFSCs), and hair matrix cells (HMCs) are believed as the key components [9,10]. It is very clear that stage transitions of HF and cyclic hair growth virtually depend on dynamic alterations of these cells among finely modulated proliferation, differentiation, and apoptosis [11][12][13]. Previously, the usefulness of cultured DPCs and HFSCs serving as models to utilize presently deposited data have been demonstrated.…”

Section: Introductionmentioning

confidence: 99%

Cultivation of Hair Matrix Cells from Cashmere Goat Skins and Exemplified Applications

Wang

Zong

et al. 2020

Animals

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A functional interpretation of filtered candidates and predicted regulatory pathways related to cashmere growth from sequencing trials needs available cell models, especially for hair matrix cells (HMCs), whose continual proliferation and differentiation result in rapid hair growth. To fulfill such goals, we herein obtained primary goat HMCs via a microdissection-based method; optimized the selection of the culture medium and coating substances for better cell maintenance; and exemplified their usefulness through examining the effects of calcium and all-trans retinoic acid (ATRA) on cells using immunoblotting, flow cytometry, and other techniques. As a result, we successfully acquired primary and passaged goat HMCs with typical keratinocyte morphology. Calcium-free RPMI (Roswell Park Memorial Institute) 1640 and MEM (minimum Eagle’s medium) outperformed normal DMEM/F12 (Dulbecco’s modified Eagle’s medium/Nutrient Mixture F-12) on long-term cell maintenance, whereas serum-free media K-SFM and EpiLife failed to support cell growth. HMCs differed molecularly and morphologically from their neighbor dermal papilla cells on expressions of feature genes, such as HOXC13, and on characteristic keratinocyte-like appearances versus fibroblast shapes, respectively. Higher calcium concentrations significantly stimulated the expression of the genes (e.g., KRT1 and IVL) involved in keratinocyte differentiation and, promoted cell proliferation. Moreover, 10−5 M ATRA obviously boosted goat HMC expansions and changed their cell cycle distributions compared to the controls. Our study shines a light on researches exploring the mechanisms underlying the growth of cashmere.

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Section: Introductionmentioning

confidence: 99%

Cultivation of Hair Matrix Cells from Cashmere Goat Skins and Exemplified Applications

Wang

Zong

et al. 2020

Animals

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“…It has been reported that Meis1 is involved in the proliferation and migration of pulmonary artery smooth muscle cells (PASMCs) in hypoxia [ 56 ]. Recently, Meis1 has been proven to act as a transcription factor to promote hair matrix cell proliferation [ 57 ], suggesting that Meis1 has a unique function on the regulation of cell proliferation. However, in this study, we found that DjMeis1 RNAi planarians displayed normal mitotic activities compared to the control groups ( Figure 3 B–E), indicating that knockdown of DjMeis1 had no effect on the proliferation of neoblasts.…”

Section: Discussionmentioning

confidence: 99%

Meis1 Controls the Differentiation of Eye Progenitor Cells and the Formation of Posterior Poles during Planarian Regeneration

Wang

Sun

Liu

et al. 2023

IJMS

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As a member of TALE family, Meis1 has been proven to regulate cell proliferation and differentiation during cell fate commitment; however, the mechanism is still not fully understood. The planarian, which has an abundance of stem cells (neoblasts) responsible for regenerating any organ after injury, is an ideal model for studying the mechanisms of tissue identity determination. Here, we characterized a planarian homolog of Meis1 from the planarian Dugesia japonica. Importantly, we found that knockdown of DjMeis1 inhibits the differentiation of neoblasts into eye progenitor cells and results in an eyeless phenotype with normal central nervous system. Furthermore, we observed that DjMeis1 is required for the activation of Wnt signaling pathway by promoting the Djwnt1 expression during posterior regeneration. The silencing of DjMeis1 suppresses the expression of Djwnt1 and results in the inability to reconstruct posterior poles. In general, our findings indicated that DjMeis1 acts as a trigger for the activation of eye and tail regeneration by regulating the differentiation of eye progenitor cells and the formation of posterior poles, respectively.

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“…We have found that D-panthenol enhanced the growth of hDPCs and hORSCs (Figures 1A and 6D), inducing the expression of cell proliferation marker (Ki67, Figure 1B) in cultured hDPCs. Cellular growth in hair follicles has been reported as a significant characteristic of the anagen phase [38][39][40][41]. The dermal papilla goes through miniaturization when hair follicles proceed to catagen and telogen [42,43], after which it recovers its original size in the course of anagen initiation.…”

Section: Discussionmentioning

confidence: 99%

Dexpanthenol Promotes Cell Growth by Preventing Cell Senescence and Apoptosis in Cultured Human Hair Follicle Cells

Shin

Kim

Choi

et al. 2021

CIMB

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Dexpanthenol (D-panthenol) is a precursor of vitamin B5 (pantothenic acid) and is widely used for dietary supplements and topical applications. D-panthenol has long been used in hair care products for the purpose of anti-hair loss, its effects and the underlying mechanisms, however, were barely reported. In this study, the effects of D-panthenol on human hair follicle cells, including dermal papilla cells (hDPCs) and outer root sheath cells (hORSCs), were investigated. D-panthenol enhanced the cell viability, increasing the cellular proliferation marker Ki67 in cultured hDPCs. The markers for apoptosis (Caspase3/9) and cell senescence (p21/p16), reported to be expressed in aged or resting phase follicles, were significantly reduced by D-panthenol. Anagen-inducing factors (ALP; β-catenin; versican), which trigger or elongate the anagen phase, were stimulated by D-panthenol. On the other hand, D-panthenol reduced TGF-β1 expressions in both mRNA and protein levels. The expression of VEGF, which is important for peripheral blood vessel activation; was up-regulated by D-panthenol treatment. In cultured hORSCs, cell proliferation and viability were enhanced, while the mRNA expression of cell senescence markers (p21/p16) was significantly down-regulated. The expressions of both VEGF and its receptor (VEGFR) were up-regulated by D-panthenol. In conclusion, our data suggest that the hair growth stimulating activity of D-panthenol was exerted by increasing the cell viability, suppressing the apoptotic markers, and elongating the anagen phase in hair follicles.

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Nuclear localization of Meis1 in dermal papilla promotes hair matrix cell proliferation in the anagen phase of hair cycle

Cited by 6 publications

References 24 publications

Cultivation of Hair Matrix Cells from Cashmere Goat Skins and Exemplified Applications

Cultivation of Hair Matrix Cells from Cashmere Goat Skins and Exemplified Applications

Meis1 Controls the Differentiation of Eye Progenitor Cells and the Formation of Posterior Poles during Planarian Regeneration

Dexpanthenol Promotes Cell Growth by Preventing Cell Senescence and Apoptosis in Cultured Human Hair Follicle Cells

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