Nuclear Localization of Annexin A1 Correlates With Advanced Disease and Peritoneal Dissemination in Patients with Gastric Carcinoma

Abstract

Annexin A1 (ANXA1) is a multifunctional molecule, which mediates various important physiologic processes depending on its subcelluar localization. The purpose of this study was to investigate the expression of ANXA1 level and its subcellular localization in paired clinical samples of gastric adenocarcinoma and adjacent normal counterpart. The study also assesses the clinical significance of ANXA1 subcelluar localization in gastric adenocarcinoma. A total of 104 paired resected gastric adenocarcinoma and corresponding normal specimens were collected in this study. Expression of ANXA1 was examined by immunohistochemical staining. Both cytoplasmic and nuclear ANXA1 expression levels and their correlation with clinicopathological parameters were assessed. ANXA1 protein expression was positive in 72 of 104 (69.2%) normal tissues and 47 of 104 (45.2%) gastric adenocarcinoma tissues. ANXA1 staining was predominantly localized in the cytoplasm in all 72 ANXA1-positive normal specimens, whereas 12 ANXA1-positive gastric adenocarcinoma specimens showed positive nuclear staining. The positive nuclear staining correlated well with serosal invasion, peritoneal dissemination and TNM stage. Cases with positive nuclear staining presented more peritoneal dissemination (41.7%, 5/12) than those with negative nuclear staining (8.7%, 8/ 92; P ¼ 0.007). A logistic regression model revealed that positive ANXA1 nuclear staining had an independent association with peritoneal dissemination (P ¼ 0.039; hazards ratio, 9.499; 95% confidence interval, 1.159-77.815). These results indicated that ANXA1 is expressed in both gastric adenocarcinoma and normal tissues. In gastric adenocarcinoma tissues

Annexin A1 regulates EGFR activity and alters EGFR-containing tumour-derived exosomes in head and neck cancers

“…This further supports a growth suppressor role for ANXA1 in HNSCC. Cleavage and secretion of ANXA1 have recently been reported [16,43]. Extracellular ANXA1 acts as ligand for the formyl peptide receptors FPR1 and FPR2, and receptor activation has been associated with increases in cellular proliferation and migration [44].…”

Prognostic Significance of Annexin A1 Expression in Pancreatic Ductal Adenocarcinoma

“…Moreover, prognostic significance of annexin A1 nuclear staining showed a lower overall survival (Lin et al, 2008). Afterwards, Fengjia ZHU et al also found that positive nuclear staining of annexin A1 in gastric cancer tissue was connected with advanced disease stage and peritoneal dissemination (Zhu et al, 2010). As a result, alteration of annexin A1 subcellular distribution may also be involved in monitoring the progress of cancer and predicting the prognosis of patients.…”

Deregulation of Annexin-A1 and Galectin-1 Expression in Precancerous Gastric Lesions: Intestinal Metaplasia and Gastric Ulcer

“…Some of them found AnxA1 and Gal-1 downregulation [39–41], while others reported increase of these proteins in the lesions and tumor tissue [42–46]. …”