Nuclear import receptors and hnRNPK mediates nuclear import and stress granule localization of SIRLOIN

Yao, Jialin; Tu, Yingfeng; Shen, Congcong; Zhou, Qiao; Xiao, Hengyi; Jia, Da; Sun, Qingxiang

doi:10.1007/s00018-021-03992-7

Cited by 3 publications

(2 citation statements)

References 53 publications

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“…The third KH domain of hnRNP-K KH3 has been shown to bind to nucleic acids as an isolated domain, although, exhibiting lower affinity than the full-length protein ( 98 ). These previous studies have observed that the KH3 domains bind to TCCC or CCCC-rich as well as CCTC C/T-rich motifs ( 98 , 99 ). Two TCCC motifs are present in the sense LincRNA-p21 AluSx1 RNA in both the 5′-junction and 3′-three-way junctions.…”

Section: Discussionmentioning

confidence: 82%

Biophysical characterisation of human LincRNA-p21 sense and antisense Alu inverted repeats

D’Souza

Mrozowich

Badmalia

et al. 2022

Nucleic Acids Research

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Human Long Intergenic Noncoding RNA-p21 (LincRNA-p21) is a regulatory noncoding RNA that plays an important role in promoting apoptosis. LincRNA-p21 is also critical in down-regulating many p53 target genes through its interaction with a p53 repressive complex. The interaction between LincRNA-p21 and the repressive complex is likely dependent on the RNA tertiary structure. Previous studies have determined the two-dimensional secondary structures of the sense and antisense human LincRNA-p21 AluSx1 IRs using SHAPE. However, there were no insights into its three-dimensional structure. Therefore, we in vitro transcribed the sense and antisense regions of LincRNA-p21 AluSx1 Inverted Repeats (IRs) and performed analytical ultracentrifugation, size exclusion chromatography, light scattering, and small angle X-ray scattering (SAXS) studies. Based on these studies, we determined low-resolution, three-dimensional structures of sense and antisense LincRNA-p21. By adapting previously known two-dimensional information, we calculated their sense and antisense high-resolution models and determined that they agree with the low-resolution structures determined using SAXS. Thus, our integrated approach provides insights into the structure of LincRNA-p21 Alu IRs. Our study also offers a viable pipeline for combining the secondary structure information with biophysical and computational studies to obtain high-resolution atomistic models for long noncoding RNAs.

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Section: Discussionmentioning

confidence: 82%

Biophysical characterisation of human LincRNA-p21 sense and antisense Alu inverted repeats

D’Souza

Mrozowich

Badmalia

et al. 2022

Nucleic Acids Research

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show abstract

“…The structure of the KH domain of hnRNPK in association with ssDNA has been solved by X-ray crystallography (hnRNPK PDB: 7CRE). It was demonstrated to associate with RNA via multiple weak interactions ( Yao et al, 2021 ). Mutations in both copies of hnRNPK in diploid cells are embryonic lethal in mice ( Gallardo et al, 2015 ), whereas mutation of a single copy causes Au–Kline syndrome characterized by hypotonia, learning disability, and delayed development ( Au et al, 1993 ).…”

Section: Membrane Associations Of Kh Domain Proteinsmentioning

confidence: 99%

Nucleic acid-binding KH domain proteins influence a spectrum of biological pathways including as part of membrane-localized complexes

Hasan,

Jeannine Brady

2024

Journal of Structural Biology: X

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Assessing the role of intrinsic disorder in RNA-binding protein function: hnRNP K as a case study

Malaney

Benitez

Zhang

et al. 2022

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Nuclear import receptors and hnRNPK mediates nuclear import and stress granule localization of SIRLOIN

Cited by 3 publications

References 53 publications

Biophysical characterisation of human LincRNA-p21 sense and antisense Alu inverted repeats

Biophysical characterisation of human LincRNA-p21 sense and antisense Alu inverted repeats

Nucleic acid-binding KH domain proteins influence a spectrum of biological pathways including as part of membrane-localized complexes

Assessing the role of intrinsic disorder in RNA-binding protein function: hnRNP K as a case study

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