Nuclear hormone receptor BmFTZ-F1 is involved in regulating the fibroin heavy chain gene in the silkworm, Bombyx mori

Zhou, Chunyan; Zha, Xingfu; Shi, Pengju; Zhao, Peng; Wang, He; Zheng, Renwen; Xia, Qingyou

doi:10.1016/j.bbagen.2016.07.025

Cited by 22 publications

(25 citation statements)

References 27 publications

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“…However, their expression was low during the IV‐M stage. Thus, the periodic synthesis of silk proteins is regulated by some relevant genes, ensuring the normal function of silk gland cells and enhancing protein synthesis during the feeding stages . The ribosomal proteins that were downregulated at the IV‐M stage might contribute to decreased protein synthesis at the translation level, which has been confirmed in a previous study .…”

Section: Discussionsupporting

confidence: 74%

“…Thus, the periodic synthesis of silk proteins is regulated by some relevant genes, ensuring the normal function of silk gland cells and enhancing protein synthesis during the feeding stages. [27,[30][31][32] The ribosomal proteins that were downregulated at the IV-M stage might contribute to decreased protein synthesis at the translation level, which has been confirmed in a previous study. [33] Therefore, the periodic synthesis of silk proteins indicated that the silk gland had a reasonable time scale for ensuring the normal growth and development of B. mori.…”

Section: Discussionmentioning

confidence: 63%

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Comparative Proteome Analysis Reveals that Cuticular Proteins Analogous to Peritrophin‐Motif Proteins are Involved in the Regeneration of Chitin Layer in the Silk Gland of Bombyx mori at the Molting Stage

et al. 2018

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The silk gland of silkworm produces silk proteins during larval development. Many studies have long focused on the silk gland of the fifth instar larvae, but few have investigated this gland at other larval stages. In the present study, the silk gland proteomes of the fourth instar and fourth molt are analyzed using liquid chromatography-tandem mass spectrometry. In total, 2654 proteins are identified from the silk gland. A high abundance of ribosomal proteins and RR-motif chitin-binding proteins is identified during day 2 of the fourth instar (IV-2) larval developmental stage, and the expression of cuticular proteins analogous to peritrophin (CPAP)-motif chitin-binding proteins is higher during the fourth molt (IV-M). In all, nine enzymes are found to be involved in the chitin regeneration pathway in the silk gland. Among them, two chitinase and two chitin deacetylases are identified as CPAP-motif proteins. Furthermore, the expression of CPAP3-G, the most abundant CPAP-motif cuticular protein in the silk gland during the IV-M stage, is investigated using western blot and immunofluorescence analyses; CPAP3-G shows a reverse changing trend with chitin in the silk gland. The findings of this study suggest that CPAP-motif chitin-binding proteins are involved in the degradation of the chitin layer in the silk gland. The data have been deposited to the ProteomeXchange with identifier PXD008677.

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Section: Discussionsupporting

confidence: 74%

Section: Discussionmentioning

confidence: 63%

Comparative Proteome Analysis Reveals that Cuticular Proteins Analogous to Peritrophin‐Motif Proteins are Involved in the Regeneration of Chitin Layer in the Silk Gland of Bombyx mori at the Molting Stage

et al. 2018

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“…In order to reveal the molecular mechanisms of transcriptional regulation, especially of the fibroin genes, regulatory elements or regions upstream of the promoters of these genes were identified [12][13][14][15][16][17][18][19][20]. Correspondingly, many factors were identified to regulate the transcription of silk protein genes, including BmFkh/SGF-1 [21], SGF-2 [22], POU-M1/SGF-3 [23], SGF-4 [13], FBF-A1 [13], FMBP-1 [17,24], BmFTZ-F1 [25], BmSage [26] and BmDimm [27]. Most of them function as activators of silk protein genes.…”

Section: Introductionmentioning

confidence: 99%

“…BmDimm can interact with BmSage to regulate the transcription of FibH by binding to the E-box element and be upregulated by Krüppel homolog 1 (BmKr-h1) by the JH-signaling pathway [27]. Both FMBP-1 and BmFTZ-F1 could repress the activity of FibH promoter by binding to the upstream elements at the cellular level, suggesting that they may be repressors of the FibH gene [24,25].…”

Section: Introductionmentioning

confidence: 99%

Transgenic Ectopic Overexpression of Broad Complex (BrC-Z2) in the Silk Gland Inhibits the Expression of Silk Fibroin Genes of Bombyx mori

Cong

Tao

Zhang

et al. 2020

Insects

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Bombyx mori silk protein genes are strictly turned on and off in different developmental stages under the hormone periodically change. The broad complex (BrC) is a transcription factor mediating 20-hydroxyecdysone action, which plays important roles during metamorphosis. Here, we observed that two isoforms of BmBrC (BmBrC-Z2 and BmBrC-Z4) exhibited contrasting expression patterns with fibroin genes (FibH, FibL and P25) in the posterior silk gland (PSG), suggesting that BmBrC may negatively regulate fibroin genes. Transgenic lines were constructed to ectopically overexpress BmBrC-Z2 in the PSG. The silk protein genes in the transgenic line were decreased to almost half of that in the wild type. The silk yield was decreased significantly. In addition, the expression levels of regulatory factors (BmKr-h1 and BmDimm) response to juvenile hormone (JH) signal were inhibited significantly. Then exogenous JH in the BmBrC-Z2 overexpressed lines can inhibit the expression of BmBrC-Z2 and activate the expression of silk protein genes and restore the silk yield to the level of the wild type. These results indicated that BmBrC may inhibit fibroin genes by repressing the JH signal pathway, which would assist in deciphering the comprehensive regulation mechanism of silk protein genes.

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“…S4c ). Ftz was reported to reduce the level silk protein gene transcription 37 ; thus, this factor might be involved in inhibiting the expression of Fib-L in BmE cells, but not in the silk gland. In any case, these findings indicate that transcriptional regulation of silk protein genes is a complex process.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide open chromatin regions and their effects on the regulation of silk protein genes in Bombyx mori

Zhang

Cheng

Jin

et al. 2017

Sci Rep

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Nucleosome-depleted open chromatin regions (OCRs) often harbor transcription factor (TF) binding sites that are associated with active DNA regulatory elements. To investigate the regulation of silk-protein genes, DNA molecules isolated from the silk glands of third-day fifth-instar silkworm larvae and embryo-derived (BmE) cells were subjected to formal dehyde-assisted isolation of regulatory elements (FAIRE) and high-throughput sequencing. In total, 68,000 OCRs were identified, and a number of TF-binding motifs were predicted. In particular, OCRs located near silk-protein genes contained potential binding sites for functional TFs. Moreover, many TFs were found to bind to clusters of OCRs upstream of silk-protein genes, and to regulate the expression of these genes. The expression of silk protein genes may be related not only to regulating TFs (such as fkh, Bmdimm, and Bmsage), but also to developmental and hormone-induced TFs (such as zen, eve, Br, and eip74ef). Elucidation of genome-wide OCRs and their regulatory motifs in silk protein genes will provide valuable data and clues for characterizing the mechanisms of transcriptional control of silk protein genes.

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Nuclear hormone receptor BmFTZ-F1 is involved in regulating the fibroin heavy chain gene in the silkworm, Bombyx mori

Cited by 22 publications

References 27 publications

Comparative Proteome Analysis Reveals that Cuticular Proteins Analogous to Peritrophin‐Motif Proteins are Involved in the Regeneration of Chitin Layer in the Silk Gland of Bombyx mori at the Molting Stage

Comparative Proteome Analysis Reveals that Cuticular Proteins Analogous to Peritrophin‐Motif Proteins are Involved in the Regeneration of Chitin Layer in the Silk Gland of Bombyx mori at the Molting Stage

Transgenic Ectopic Overexpression of Broad Complex (BrC-Z2) in the Silk Gland Inhibits the Expression of Silk Fibroin Genes of Bombyx mori

Genome-wide open chromatin regions and their effects on the regulation of silk protein genes in Bombyx mori

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