Nuclear granules recognized by some monoclonal antibodies against intermediate filament protein locate on chromosomes during mitosis

Kamei, Hiroya

doi:10.1016/0309-1651(89)90152-5

Cited by 5 publications

(2 citation statements)

References 26 publications

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“…PCR was performed for various cycles using WT1 primers (sense primer for exon 7, 5′-GGCATCTGAGACCAGT-GAGAA-3′; antisense primer for exon 10, 5′-GAGAGT-CAGACTTGAAAGCAGT-3′) with serial dilutions of the cDNA prepared from total RNA of K562 human leukemic cells which highly express WT1. PCR amplification for 35,29,25 Fig. 1, A-D.…”

Section: Methodsmentioning

confidence: 99%

Expression of the Wilms' Tumor Gene WT1 in Solid Tumors and Its Involvement in Tumor Cell Growth

Oji

Ogawa

Tamaki

et al. 1999

Japanese Journal of Cancer Research

197

172

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Section: Methodsmentioning

confidence: 99%

Expression of the Wilms' Tumor Gene WT1 in Solid Tumors and Its Involvement in Tumor Cell Growth

Oji

Ogawa

Tamaki

et al. 1999

Japanese Journal of Cancer Research

197

172

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“…Chronic myeloid leukemia K562 [ 43 ], acute myeloid leukemia Kasumi-1 [ 44 ], and acute promyelocytic leukemia HL60 [ 45 ] cell lines were cultured in RPMI1640 medium supplemented with 10% FBS. Lung cancer LU99B [ 46 ], gastric cancer AZ-521 [ 47 ], fibrosarcoma HT-1080 [ 48 ] and human embryonic kidney 293 [ 49 ] cell lines were cultured in Dulbecco’s modified essential medium supplemented with 10% FBS. All cell lines used in this study were obtained from Japan Health Sciences Foundation (Osaka, Japan).…”

Section: Methodsmentioning

confidence: 99%

Identification of a Novel C-Terminal Truncated WT1 Isoform with Antagonistic Effects against Major WT1 Isoforms

et al. 2015

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The Wilms’ tumor gene WT1 consists of 10 exons and encodes a zinc finger transcription factor. There are four major WT1 isoforms resulting from alternative splicing at two sites, exon 5 (17AA) and exon 9 (KTS). All major WT1 isoforms are overexpressed in leukemia and solid tumors and play oncogenic roles such as inhibition of apoptosis, and promotion of cell proliferation, migration and invasion. In the present study, a novel alternatively spliced WT1 isoform that had an extended exon 4 (designated as exon 4a) with an additional 153 bp (designated as 4a sequence) at the 3’ end was identified and designated as an Ex4a(+)WT1 isoform. The insertion of exon 4a resulted in the introduction of premature translational stop codons in the reading frame in exon 4a and production of C-terminal truncated WT1 proteins lacking zinc finger DNA-binding domain. Overexpression of the truncated Ex4a(+)WT1 isoform inhibited the major WT1-mediated transcriptional activation of anti-apoptotic Bcl-xL gene promoter and induced mitochondrial damage and apoptosis. Conversely, suppression of the Ex4a(+)WT1 isoform by Ex4a-specific siRNA attenuated apoptosis. These results indicated that the Ex4a(+)WT1 isoform exerted dominant negative effects on anti-apoptotic function of major WT1 isoforms. Ex4a(+)WT1 isoform was endogenously expressed as a minor isoform in myeloid leukemia and solid tumor cells and increased regardless of decrease in major WT1 isoforms during apoptosis, suggesting the dominant negative effects on anti-apoptotic function of major WT1 isoforms. These results indicated that Ex4a(+)WT1 isoform had an important physiological function that regulated oncogenic function of major WT1 isoforms.

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Location of nuclear antigen(s) recognized by DSB389 MAb, a monoclonal antibody against desmin, observed by confocal laser scanning fluorescence microscopy

Kamei

1990

Cell Biology International Reports

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The location of antigen(s) of DSB389 MAb, a monoclonal antibody which was raised against an intermediate filament protein, desmin, was investigated in HeLa S3 cells by indirect immuno-electron microscopy and by confocal laser scanning fluorescence microscopy. At interphase, the antigen(s) locates mainly in the intra-nuclear space adjacent to chromatin and sometimes near the nuclear periphery. At mitosis, they locate at the periphery of the chromosomes -first at each chromosome, then around the mass of chromosomes. The antigen(s) are thought to be a component of one type of nuclear matrix which is present outside both chromatin and chromosomes and which has some structural role(s) in organizing them in the nucleus or in the nuclear region.

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Nuclear granules recognized by some monoclonal antibodies against intermediate filament protein locate on chromosomes during mitosis

Cited by 5 publications

References 26 publications

Expression of the Wilms' Tumor Gene WT1 in Solid Tumors and Its Involvement in Tumor Cell Growth

Expression of the Wilms' Tumor Gene WT1 in Solid Tumors and Its Involvement in Tumor Cell Growth

Identification of a Novel C-Terminal Truncated WT1 Isoform with Antagonistic Effects against Major WT1 Isoforms

Location of nuclear antigen(s) recognized by DSB389 MAb, a monoclonal antibody against desmin, observed by confocal laser scanning fluorescence microscopy

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