Nuclear genome size analysis of<i>Agave tequilana</i>Weber

Palomino, Guadalupe; Doležel, Jaroslav; Méndez, Ignacio; Rubluo, A.

doi:10.1080/00087114.2003.10589305

Cited by 42 publications

(48 citation statements)

References 29 publications

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“…The results found in this study agree with those of PALOMINO et al (2003), who analysed the nuclear content of several cultivars of Agave tequilana using flow cytometry; results showed that the diploid cultivars "Lineño", "Azul Listado", "Azul", "Moraleño" and "Sigüin" had similar genome size, in addition the cultivar "Pata de Mula" had the largest genome size within all the diploid cultivars. The triploid "Bermejo" (3x) and tetra- ploid "Chato" (4x) had significant larger genome size than all the diploids.…”

Section: Karyotypic Analysis Of Eight Agave Tequilana Cultivars Is Susupporting

confidence: 81%

“…The research on chromosome number and karyotypes has revealed aspects on the inter-and intra-specific variation in Agave species (BANER- JEE and SHARMA 1988;1989;CASTORENA-SÁNCHEZ et al 1991;PALOMINO et al 2005); DNA nuclear content or genome size (PALOMINO et al 2003;ZONNEVELD 2003) and molecular markers such as randomly amplified polymorphic DNA (RAPD; GIL-VEGA et al 2001), and amplification fragment length polymorphism (AFLP; BARRAZA-MORALES et al 2006;GIL-VEGA et al 2006) have been used to elucidate its genetics.…”

Section: Introductionmentioning

confidence: 99%

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Karyotype studies in cultivars of Agave tequilana Weber

2008

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Section: Karyotypic Analysis Of Eight Agave Tequilana Cultivars Is Susupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Karyotype studies in cultivars of Agave tequilana Weber

2008

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“…This means that, once collected, plant material needs to be analyzed within a short time interval, constituting an important limitation for determining the nuclear DNA amount of plants growing in remote places and/or collected during long expeditions (Suda and Trávníček 2006), which is the case for most Echinops. Moreover, other parameters, such as leaf age and/or its position on the plant, can lead to variations in DNA amount measurements (Palomino et al 2003), which are probably in part explained by differences in secondary metabolism. Protocols for eluding the fresh material requirement by the use of frozen or dehydrated tissues (silica gel-preserved or herbarium vouchers) are receiving increased attention (Suda and Trávníček 2006;Popp et al 2008;Cires et al 2009;Dušková et al 2010;Temsch and Greilhuber 2010;Bainard et al 2011), although their accuracy for drawing comparisons between individuals of close genome sizes is still not clear (Cires et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Genome size and chromosome number in Echinops (Asteraceae, Cardueae) in the Aegean and Balkan regions: technical aspects of nuclear DNA amount assessment and genome evolution in a phylogenetic frame

et al. 2012

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This work focuses on the representatives of genus Echinops (Asteraceae, Cardueae) in the Aegean and Balkan regions, from the perspective of their genome evolution. Chromosome numbers were determined by orcein staining in 14 populations of nine taxa, and DNA contents were assessed by flow cytometry in 24 populations of nine taxa. A molecular phylogeny based on the internal transcribed spacer (ITS) and trnL-trnF and including first sequences for two taxa (Echinops sphaerocephalus subsp. taygeteus and E. spinosissimus subsp. neumayeri) provided a framework for discussing genome changes. From a methodological point of view, similar C-DNA value estimates were obtained when measuring, for a same population, fresh leaves from adult plants collected in the field and from cultivated seedlings. Conversely, despite giving the appearance of being correct (e.g., low coefficient of variation), genome size assessed using silica gel-preserved material differs significantly from values obtained for the same populations with fresh material. Nevertheless, silica gel-preserved material may still provide rough estimates of genome size for, e.g., inferring ploidy level. Suitablenon-silica gel-based-DNA amounts assessed for 23 populations range from 2C = 6.52 pg (E. spinosissimus subsp. neumayeri) to 2C = 9.37 pg (E. bannaticus). Chromosome counts were established for the first time for Echinops graecus (2n = 32), E. sphaerocephalus subsp. albidus (2n = 32), E. sphaerocephalus subsp. taygeteus (2n = ca. 30), and E. spinosissimus subsp. neumayeri (2n = 28). Genome size and chromosome number are confirmed as crucial parameters for deciphering lineage diversification within the genus Echinops.

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“…Correlation between genome size and ploidy level is common in plants (Palomino et al, 2003) and similar highly significant correlations were also found in buffalograss (Bouteloua dactyloides) (Johnson et al, 1998) and fine fescues (Festuca spp.) (Huff and Palazzo, 1998).…”

Section: Discussionmentioning

confidence: 66%

Determination of DNA content and relative 2C genome sizes of some promising commercial varieties of sugarcane using flow cytometer

Mondal¹,

De²

2017

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In this study, 2C deoxyribonucleic acid (DNA) content and the genome sizes (in pictograms [pg] and megabase pairs [Mbp], respectively) of 19 promising commercial varieties of sugarcane, the derivatives of man-made interspecific hybrids between cultivated and wild species were analyzed using flow cytometry. In this work, 2C nuclear DNA content was determined. Knowing the 2C nuclear DNA content, the unknown chromosome numbers of the varieties could be predicted. Large differences (65% variation) in DNA content (2C) of 19 varieties were detected, ranging, from 3.80 to 10.96 pg, which corresponds to a genome size ranging from 3724.00 to 10740.80 Mbp due to the variation of ploidy level and are considered the most complex genomes among crop plants. However, the relationship between chromosome number and genome size was highly significant (P < 0.001). In this study, internode diameter, sugar juice content and cane yield/ha are also positively correlated with DNA content. The estimated genome sizes would also yield information critical for sugarcane breeding and genome sequencing programs.

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Nuclear genome size analysis ofAgave tequilanaWeber

Cited by 42 publications

References 29 publications

Karyotype studies in cultivars of Agave tequilana Weber

Karyotype studies in cultivars of Agave tequilana Weber

Genome size and chromosome number in Echinops (Asteraceae, Cardueae) in the Aegean and Balkan regions: technical aspects of nuclear DNA amount assessment and genome evolution in a phylogenetic frame

Determination of DNA content and relative 2C genome sizes of some promising commercial varieties of sugarcane using flow cytometer

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