Nuclear fusion and irregular cytokinesis in binucleate and tetraploid cells ofVicia faba after caffeine treatment

Röper, W.

doi:10.1007/bf01953082

Cited by 7 publications

(4 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The phenomenon has been observed in Vicia faba (Roper 1976) and Allium cepa (Gimenez-Martin et al 1968) with binucleate cells obtained by a pulse treatment with caffeine.…”

Section: Disappearance Of Binucleate Cellsmentioning

confidence: 78%

Methyl-3 hydroxy-6 quinazoline dione-2-4, a new compound for labelling plant cells. Application to the study of two inhibitors of cytokinesis.

Deysson¹,

Lasselain²,

Pareyre³

1980

CYTOLOGIA

View full text Add to dashboard Cite

“…The phenomenon has been observed in Vicia faba (Roper 1976) and Allium cepa (Gimenez-Martin et al 1968) with binucleate cells obtained by a pulse treatment with caffeine.…”

Section: Disappearance Of Binucleate Cellsmentioning

confidence: 78%

Methyl-3 hydroxy-6 quinazoline dione-2-4, a new compound for labelling plant cells. Application to the study of two inhibitors of cytokinesis.

Deysson¹,

Lasselain²,

Pareyre³

1980

CYTOLOGIA

View full text Add to dashboard Cite

“…Compared with differentiating cells, these stem cells may have long cell cycle times (Clowes 1961). If induced binucleate cells are able to cellularize as the effect of caffeine recedes (Roper 1976), there could be a link between long cell cycles and resistance to doubling with caffeine. Doubling of cells in the apical meristem with caffeine may require longer treatments than those used here in order to preserve the binucleate condition between consecutive mitoses (a double hit).…”

Section: Discussionmentioning

confidence: 99%

“…Sister nuclei may then become polyploid either through nuclear fusion or, more usually, by entering mitosis together on a common metaphase plate (Gimenez-Martin et al 1968). Either way, treatment with caffeine over several cell cycles will generate a population of cells with two or more nuclei of increasing ploidy (Gimenez-Martin et al 1968;Liu et al 1995;Roper 1976;Kihlman and Levan 1949). A preliminary trial showed that wheat root tips treated with 1 g -~-l caffeine contained many binucleate cells with polyploid nuclei (J.B. Thomas, unpublished).…”

mentioning

confidence: 96%

See 1 more Smart Citation

Chromosome doubling of haploids of common wheat with caffeine

Thomas¹,

Chen²,

Howes³

1997

Genome

View full text Add to dashboard Cite

Treatment of dividing plant cells with caffeine inhibits their cytokinesis, thereby inducing the formation of binucleate cells that contain polyploid nuclei. This study was undertaken to determine whether caffeine treatments would induce chromosome doubling and seed set in haploids of common wheat (Triticum aestivum L. em. Thell.) through the further development of diploid nuclei. Ten sterile wheat haploid plants, obtained through the agency of corn (Zea mays L.) pollination, were multiplied by vegetative propagation (subdivision of well-tillered plants) to produce about 50 crowns per haploid. Washed and trimmed crowns were treated with 0.3, 1.0, 3.0, and 10.0 g∙L−1 caffeine for 3, 6, 12, and 24 h. While treatment with 0.3 g∙L−1 caffeine did not restore fertility, higher concentrations of caffeine resulted in pollen shedding and substantial seed set compared with untreated controls. Many combinations of caffeine concentration and duration produced comparable results; however, treatment with 3 g∙L−1 for 24 h was the most effective caffeine treatment on the basis of the number of seeds recovered, as well as the size and incidence of fertile sectors. Compared with a standard colchicine treatment (3-h immersion in 2 g∙L−1 colchicine dissolved in 0.5% dimethyl sulfoxide), all caffeine treatments produced fewer seeds, principally because colchicine-doubled sectors were often large, while caffeine-doubled sectors were numerous but generally small. In conclusion, caffeine produced useful numbers of seeds for all clones; gliadin banding patterns and chromosome counts indicated that genetic and cytogenetic stability of the doubling process were equal or superior for caffeine compared with colchicine.

show abstract

Interaction of Cell-Wall Formation and Cell Division in Higher Plant Cells

Meyer

Herth

1982

Cellulose and Other Natural Polymer Systems

View full text Add to dashboard Cite

Nuclear fusion and irregular cytokinesis in binucleate and tetraploid cells ofVicia faba after caffeine treatment

Cited by 7 publications

References 4 publications

Methyl-3 hydroxy-6 quinazoline dione-2-4, a new compound for labelling plant cells. Application to the study of two inhibitors of cytokinesis.

Methyl-3 hydroxy-6 quinazoline dione-2-4, a new compound for labelling plant cells. Application to the study of two inhibitors of cytokinesis.

Chromosome doubling of haploids of common wheat with caffeine

Interaction of Cell-Wall Formation and Cell Division in Higher Plant Cells

Contact Info

Product

Resources

About