Nuclear Factor-Kappa B and Other Oxidative Stress Biomarkers in Serum of Autistic Children

Abdel-Salam, Omar M.E.; Youness, Eman R.; Mohammed, Nadia A.; Elhamed, Walaa A. Abu

doi:10.4236/ojmip.2015.51002

Cited by 22 publications

(7 citation statements)

References 60 publications

(41 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This enzyme possesses an esterase and lactonase activities and is involved in xenobiotic metabolism (La Du, 1992) and in protection against oxidative and inflammatory events (Ng et al, 2008). The activity of PON-1 decreases in serum from patients with chronic liver disease (Keskin et al, 2009) and in a number of neurological disorders (Menini and Gugliucci, 2014;Abdel-Salam et al, 2015). We in addition demonstrated that brain butrylcholinesterase (BChE) activity was significantly inhibited by EtOH.…”

Section: Discussionmentioning

confidence: 88%

Methylene Blue Protects Against Acute Ethanol-Induced Oxidative Stress and Organ Damage

et al. 2021

Self Cite

View full text Add to dashboard Cite

Ethanol (EtOH) intake is an important global health problem which affects many organs such as the brain, liver and stomach. The aim of the study was to examine the effect of the redox dye methylene blue (MethyB) on oxidative stress and histologic damage to the liver, gastric mucosa, and brain induced high dose ethanol (EtOH). Male rats were treated with EtOH (2 ml/rat, 96%) via intragastric route (for two consecutive days). MethyB (20 or 40 mg/kg, intraperitoneally) was given immediately after EtOH administration. The control group received saline. Rats were euthanized three hours after the last treatment. Brain and liver levels of malondialdehyde (MDA), reduced glutathione (GSH), and paraoxonase-1 (PON-1) as well as brain 5-lipoxygenase (5-LOX) and butyrylcholinesterase (BChE) were determined. Histopathological assessment of brain, liver and gastric damage was done. Results indicated that compared to saline treated animals, EtOH caused significant increase in MDA, along with decreased GSH and PON-1 activity in brain and liver. Additionally, it significantly increased 5-LOX and decreased brain BChE activity. The EtOH group showed the presence of dead and red neurons, and damage of glial cells. The liver exhibited vacuolar degeneration, apoptotic hepatocytes and foci of necrosis. The gastric mucosa showed areas of tissue damage, mucosal atrophy, and loss of normal architecture of glandular cells. The EtOH induced biochemical and histopathological alterations were alleviated after treatment with MethyB at a dose-dependent manner. These results demonstrate that MethyB is able to protect against from acute effects of EtOH on brain, liver and gastric tissue via an antioxidant action. MethyB might be of value in reducing tissue injury in acute EtOH intoxication.

show abstract

Section: Discussionmentioning

confidence: 88%

Methylene Blue Protects Against Acute Ethanol-Induced Oxidative Stress and Organ Damage

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Furthermore, sialic acid modification of TLR4 can regulate the nuclear factor kappa-B (NF-κB) signaling pathway and lead to altered the protein expression level of cytokines ( 29 , 30 ). Significantly increased serum NF-κB concentration was found in children with ASD ( 31 ). Animal experiments have shown that TLR4 expression is increased in mice with maternal LPS exposure, and their offspring exhibit ASD-like behaviors ( 32 ).…”

Section: Discussionmentioning

confidence: 99%

Correlation Between Sialidase NEU1 mRNA Expression Changes in Autism Spectrum Disorder

Zhang

Gu²,

He³

et al. 2022

Front. Psychiatry

View full text Add to dashboard Cite

Abnormal alterations in enzymes functioned in sialic acid modifications may be associated with ASD. In order to study the differences in peripheral blood sialidase (neuraminidase 1; NEU1) mRNA expression between autism spectrum disorder (ASD) children and healthy control, and to examine the correlation between NEU1 mRNA expression and the main behavioral phenotypes in children with ASD, we performed RT-qPCR to measure NEU1 mRNA expression in peripheral blood of 42 children with ASD and 42 healthy controls. In addition, we used the Autism Diagnostic Observation Schedule, Second Edition (ADOS-2) to measure and evaluate the behavioral phenotypes of children with ASD. Our results showed that NEU1 mRNA in the ASD group was significantly higher than in the control group (P < 0.0001). In addition, the ADOS-2 diagnostic scores of 42 children with ASD were correlated with their NEU1 mRNA expression results (R = 0.344, P = 0.0257). Moreover, in general, NEU1 mRNA expression was also positively correlated with the Social Affect (SA) of ADOS-2 (R = 0.3598, P = 0.0193) but not with the Restricted and Repetitive Behavior (RRB) (R = 0.15, P = 0.3432). Our results indicated that sialidase NEU1 mRNA was significantly increased in children with ASD, and its expression was correlated with the SA of children with ASD, which suggested that sialidase NEU1 may affect the SA of ASD. Our data highlighted the potential of NEU1 expression change may play an important role in ASD disease and lay the foundation for further studies on the relationship between NEU1 and ASD.

show abstract

“…Kishi et al (2016) reported that genetically reducing NF-κB signaling in Mecp2-null mice not only ameliorated cortical callosal projection neurons and dendritic complexity, but also substantially extended their normally shortened lifespan [31]. In studies with human samples, several reports indicate that NF-κB concentrations were elevated in the serum of subjects with ASD and postmortem samples of orbitofrontal cortex tissues [61,62].…”

Section: Discussionmentioning

confidence: 99%

Whole Exome Sequencing Identifies Novel De Novo Variants Interacting with Six Gene Networks in Autism Spectrum Disorder

Kim

Lim

et al. 2020

Genes

View full text Add to dashboard Cite

Autism spectrum disorder (ASD) is a highly heritable condition caused by a combination of environmental and genetic factors such as de novo and inherited variants, as well as rare or common variants among hundreds of related genes. Previous genome-wide association studies have identified susceptibility genes; however, most ASD-associated genes remain undiscovered. This study aimed to examine rare de novo variants to identify genetic risk factors of ASD using whole exome sequencing (WES), functional characterization, and genetic network analyses of identified variants using Korean familial dataset. We recruited children with ASD and their biological parents. The clinical best estimate diagnosis of ASD was made according to the Diagnostic and Statistical Manual of Mental Disorders (DSM-5TM), using comprehensive diagnostic instruments. The final analyses included a total of 151 individuals from 51 families. Variants were identified and filtered using the GATK Best Practices for bioinformatics analysis, followed by genome alignments and annotation to the reference genome assembly GRCh37 (liftover to GRCh38), and further annotated using dbSNP 154 build databases. To evaluate allele frequencies of de novo variants, we used the dbSNP, gnomAD exome v2.1.1, and genome v3.0. We used Ingenuity Pathway Analysis (IPA, Qiagen) software to construct networks using all identified de novo variants with known autism-related genes to find probable relationships. We identified 36 de novo variants with potential relations to ASD; 27 missense, two silent, one nonsense, one splice region, one splice site, one 5′ UTR, and one intronic SNV and two frameshift deletions. We identified six networks with functional relationships. Among the interactions between de novo variants, the IPA assay found that the NF-κB signaling pathway and its interacting genes were commonly observed at two networks. The relatively small cohort size may affect the results of novel ASD genes with de novo variants described in our findings. We did not conduct functional experiments in this study. Because of the diversity and heterogeneity of ASD, the primary purpose of this study was to investigate probable causative relationships between novel de novo variants and known autism genes. Additionally, we based functional relationships with known genes on network analysis rather than on statistical analysis. We identified new variants that may underlie genetic factors contributing to ASD in Korean families using WES and genetic network analyses. We observed novel de novo variants that might be functionally linked to ASD, of which the variants interact with six genetic networks.

show abstract

Nuclear Factor-Kappa B and Other Oxidative Stress Biomarkers in Serum of Autistic Children

Cited by 22 publications

References 60 publications

Methylene Blue Protects Against Acute Ethanol-Induced Oxidative Stress and Organ Damage

Methylene Blue Protects Against Acute Ethanol-Induced Oxidative Stress and Organ Damage

Correlation Between Sialidase NEU1 mRNA Expression Changes in Autism Spectrum Disorder

Whole Exome Sequencing Identifies Novel De Novo Variants Interacting with Six Gene Networks in Autism Spectrum Disorder

Contact Info

Product

Resources

About