Nuclear factor I can functionally replace transcription factor Sp1 in a U2 small nuclear RNA gene enhancer

Janson, L.; Weller, P A; Pettersson, Ulf

doi:10.1016/0022-2836(89)90349-5

Cited by 27 publications

(29 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The octamer site is also important for transcription of the snRNA genes although their expression is not restricted to lymphoid cells (Mattaj et al, 1985;Ares et al, 1987;Ciliberto et al, 1987;Janson et al, 1987Janson et al, , 1989Dahlberg and Schenborn, 1988;Tanaka et al, 1988). Transcription of human U2 genes is controlled by a proximal sequence element (PSE) and a distal sequence element (DSE) which is also called the U2 enhancer (Westin et al, 1984;Ares et al, 1985;Mangin et al, 1986; for review see Dahlberg and Lund, 1987).…”

Section: Introductionmentioning

confidence: 99%

“…Transcription of human U2 genes is controlled by a proximal sequence element (PSE) and a distal sequence element (DSE) which is also called the U2 enhancer (Westin et al, 1984;Ares et al, 1985;Mangin et al, 1986; for review see Dahlberg and Lund, 1987). The basic enhancer unit of the human U2 snRNA gene contains Spl binding sites combined with an octamer sequence (Janson et al, 1987(Janson et al, , 1989Ares et al, 1987). Transient expression experiments in HeLa cells have shown that there is an absolute requirement of the octamer motif for U2 transcription (Ares et al, 1987;Janson et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

“…The basic enhancer unit of the human U2 snRNA gene contains Spl binding sites combined with an octamer sequence (Janson et al, 1987(Janson et al, , 1989Ares et al, 1987). Transient expression experiments in HeLa cells have shown that there is an absolute requirement of the octamer motif for U2 transcription (Ares et al, 1987;Janson et al, 1989). Although an Spl binding site alone is unable to stimulate transcription, its deletion results in a 5-fold reduction in transcription (Janson et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

“…Transient expression experiments in HeLa cells have shown that there is an absolute requirement of the octamer motif for U2 transcription (Ares et al, 1987;Janson et al, 1989). Although an Spl binding site alone is unable to stimulate transcription, its deletion results in a 5-fold reduction in transcription (Janson et al, 1989). Recently, it has been demonstrated that SpI and Oct-I bind to the U2 enhancer in a co-operative fashion suggesting that these two factors interact physically (Janson and Pettersson, 1990).…”

Section: Introductionmentioning

confidence: 99%

“…The involvement of octamer motifs in the activation of genes which are expressed in a tissue specific and ubiquitous manner presents an enigma since it appears from previous studies that the ubiquitous and the lymphoid specific octamer binding factors bind with identical affinity to any given octamer motif (Scheidereit et al, 1987 (Janson et al, 1989), were transfected together with an expression vector encoding Oct-2A into HeLa cells. As a positive control another reporter gene, OCTA(2), which carries a lymphoid specific promoter (Miller et al, 1988) Figure 1B shows that the transcription activities of the two U2 constructs did not change significantly when increasing amounts of Oct-2A expression vector were introduced into the cell (Figure iB, lanes 1-4 and lanes 6-9).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Both Oct-1 and Oct-2A contain domains which can activate the ubiquitously expressed U2 snRNA genes.

et al. 1991

View full text Add to dashboard Cite

The U2 snRNA genes, which are transcribed by RNA polymerase II at high levels in all tissues examined, require both a distal and a proximal sequence element for efficient expression. The distal sequence element which has many properties in common with transcriptional enhancers contains, in addition to Sp1 binding sites, an octamer binding site which mediates activation through interactions with the ubiquitous transcription factor Oct‐1. In the present study we have attempted to answer the question whether Oct‐1 contains a unique activating domain which is required for activation of snRNA genes or whether ubiquitously expressed and lymphoid specific octamer binding factors both have the capacity to activate snRNA transcription. Our results show that in the presence of Oct‐1, overexpression of Oct‐2A in HeLa or COS1 cells neither inhibits nor stimulates transcription of U2 constructions which contain octamer binding sites with or without an adjacent Sp1 binding site. Moreover, an Oct‐2A‐‐GAL4 fusion protein in which the DNA binding domain of Oct‐2A was substituted for by the one of the yeast transcription activator GAL4 activates transcription of a human U2 snRNA gene in which the octamer binding site was replaced by a GAL4 binding site. From the results it is concluded that both Oct‐1 and Oct‐2A contain domains which can activate the ubiquitously expressed U2 snRNA genes.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Both Oct-1 and Oct-2A contain domains which can activate the ubiquitously expressed U2 snRNA genes.

et al. 1991

View full text Add to dashboard Cite

show abstract

Cell-type-specific transcription factor interactions with cis-elements present in the mouse LDH/C proximal promoter region

Yang

Riley²,

Thomas³

1998

J. Exp. Zool.

View full text Add to dashboard Cite

Spliceosomal snRNAs, the Essential Players in pre‐mRNA Processing in Eukaryotic Nucleus: From Biogenesis to Functions and Spatiotemporal Characteristics

Su,

Wu,

Chen

et al. 2024

Advanced Biology

View full text Add to dashboard Cite

Spliceosomal small nuclear RNAs (snRNAs) are a fundamental class of non‐coding small RNAs abundant in the nucleoplasm of eukaryotic cells, playing a crucial role in splicing precursor messenger RNAs (pre‐mRNAs). They are transcribed by DNA‐dependent RNA polymerase II (Pol II) or III (Pol III), and undergo subsequent processing and 3′ end cleavage to become mature snRNAs. Numerous protein factors are involved in the transcription initiation, elongation, termination, splicing, cellular localization, and terminal modification processes of snRNAs. The transcription and processing of snRNAs are regulated spatiotemporally by various mechanisms, and the homeostatic balance of snRNAs within cells is of great significance for the growth and development of organisms. snRNAs assemble with specific accessory proteins to form small nuclear ribonucleoprotein particles (snRNPs) that are the basal components of spliceosomes responsible for pre‐mRNA maturation. This article provides an overview of the biological functions, biosynthesis, terminal structure, and tissue‐specific regulation of snRNAs.

show abstract

Nuclear factor I can functionally replace transcription factor Sp1 in a U2 small nuclear RNA gene enhancer

Cited by 27 publications

References 47 publications

Both Oct-1 and Oct-2A contain domains which can activate the ubiquitously expressed U2 snRNA genes.

Both Oct-1 and Oct-2A contain domains which can activate the ubiquitously expressed U2 snRNA genes.

Cell-type-specific transcription factor interactions with cis-elements present in the mouse LDH/C proximal promoter region

Spliceosomal snRNAs, the Essential Players in pre‐mRNA Processing in Eukaryotic Nucleus: From Biogenesis to Functions and Spatiotemporal Characteristics

Contact Info

Product

Resources

About