Nuclear estrogen and progesterone receptors in the oviduct of heifers under natural and superovulated estrous cycles

Valle, Guilherme Ribeiro; Cassali, Geovanni Dantas; Nogueira, José Carlos; Castro, Antônio Carlos Leal de; Reis, Adelina M.; Cardoso, Fábio Maurício; Figueiredo, Cláudia Beatriz Oliveira; Nascimento, Evaldo

doi:10.1016/j.anireprosci.2006.08.026

Cited by 24 publications

(25 citation statements)

References 15 publications

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“…The E2 effect on the oviductal ERα and PR concentrations showed a biphasic course: An initial reduction of receptors was followed by restoration of the receptor concentration. In contrast, Valle et al (2007) found no correlation between the decrease and increase of ERα and the E2 concentration in the circulation.…”

Section: Discussioncontrasting

confidence: 42%

“…Our results confirm the findings of Brenner and Slayden (1994), who assume that steroid hormone receptors in the stromal cells of the primate oviduct contribute to the regulation of the activity of the oviductal ciliated cells. On the other hand, Valle et al (2007) found no different immunostaining of ciliated and secretory cells, and they maintain that steroid hormone receptors are present in both cell types. Furthermore, we found a weak positive expression for PR in the nuclei and cytoplasm of ciliated cells of the follicular phase, whereas they were negative in the mid-luteal phase.…”

Section: Discussionmentioning

confidence: 93%

“…Lipophilic steroids pass easily through the cell membrane (Giorgi 1980) and bind to specific cytosolic receptors (Chan and O'Malley 1976), which are translocated to the cell nucleus, interact with the chromatin, and induce the transcription of specific hormone-dependent genes (Grody et al 1982). Previous studies have shown that during normal estrous cycles, 17β-estradiol (E2) stimulates the production of ERα and PR, whereas in some species, progesterone (P4) causes an inhibitory effect on the synthesis of its own receptor (Meikle et al 2001;Reinhart et al 2003;Wu et al 1996;Valle et al 2007). …”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Cellular Expression and Localization of Estrogen Receptor α and Progesterone Receptor mRNA in the Bovine Oviduct Combining Laser-Assisted Microdissection, Quantitative PCR, and In Situ Hybridization

Kenngott

Vermehren

Sauer

et al. 2011

J Histochem Cytochem.

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The importance of using techniques that allow the study of pure populations of cells has been increasingly recognized. The authors used laser-assisted microdissection (LAM) in combination with quantitative real-time PCR (qPCR) to assess the relative expression of mRNAs encoding estrogen receptor α (ERα) and progesterone receptor (PR) in the different compartments of the bovine oviduct (epithelium, stroma, smooth muscle coat) during the follicular and mid-luteal phases of the estrus cycle. The localization of receptor mRNA was further studied using non-radioactive in situ hybridization (NISH). A special focus was on whether formalin fixation and paraffin embedding influence the quality and quantity of mRNA obtained from microdissected material. Distinct cyclic changes of the mRNA in the bovine oviduct were observed with elevated levels of PR mRNA transcripts in the epithelium and smooth muscle coat during the follicular phase. The expression of PR mRNA did not vary significantly in the stroma of the bovine oviduct during follicular and mid-luteal phases. In conclusion, the authors found that LAM with qPCR can precisely locate and accurately quantify mRNA expression in specific cell populations from formalin-fixed and paraffin-embedded oviductal tissue.

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Section: Discussioncontrasting

confidence: 42%

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

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Cellular Expression and Localization of Estrogen Receptor α and Progesterone Receptor mRNA in the Bovine Oviduct Combining Laser-Assisted Microdissection, Quantitative PCR, and In Situ Hybridization

Kenngott

Vermehren

Sauer

et al. 2011

J Histochem Cytochem.

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“…The distribution and heterogeneity of mast cells (Küther et al 1998;Eren et al 1999, Ozen et al 2002Vale et al 2009) and MHC II + cells (HLA-DP like, H42A) (Eren et al 2008(Eren et al , 2009 were studied previously in the genital tract of cows. In that study (Eren et al 2008) mast cell-like MHC II + cells were also observed in the sections prepared from oviduct, uterus and vagina.…”

Section: Discussionmentioning

confidence: 99%

MHC class II expression by mast cells in the genital tract of cows

et al. 2010

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This work examined the presence of MHC class II molecules expressing mast cells in oviduct, uterus and vaginal tissues in cows. The tissue samples of five cows were collected from a local slaughterhouse. Toluidine blue pH 0.5 (TB) and avidin-biotin peroxidase complex (ABC) staining procedures applied to adjacent sections from tissue samples. It was determined that some TB + cells were also gave positive reaction with strept ABC staining for MHC II molecules. To our knowledge this is the first evidence indicating the presence of MHC class II molecules expressing mast cells in the cow.

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“…İnek oviduktunda yapılan çalışmalarda (2,19), mast hücre sayısının hem foliküler hem de luteal dönemde, istmusda ampullaya göre fazla olduğundan, luteal fazda ise foliküler faza göre her iki bölgede de artış gösterdiğinden bahsedilmektedir. İnek oviduktunda (16) ve ovaryumunda (17) daha önce yapılan çalışmalarda Ab(+)/SO(-) mast hücrelerine rastlanmıştır.…”

Section: Introductionunclassified

Domuz oviduktunda mast hücreleri üzerinde ışık ve elektron mikroskobik çalışmalar

BAYRAKTAROĞLU¹

2014

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Özet: Bu çalışma; domuzlarda oviduktun değişik bölümlerinde bulunan mast hücrelerinin boyanma özelliklerini, ışık ve elektron mikroskobik yapılarıyla foliküler ve luteal fazlardaki sayısal dağılımlarını belirlemek amacıyla yapıldı. Çalışmada 6 adet sağlıklı, erişkin dişi domuzdan alınan ovidukt, materyal olarak kullanıldı. Domuz oviduktunun her üç bölgesindeki mast hücrelerinde Ab (Alcian blue) (+)/SO (Safranin O) (-) boyanma görüldü. Çalışmada domuz oviduktu üzerinde yapılan hücre sayımı ve istatistiksel sonuçlarına göre foliküler dönemde, birim alandaki mast hücresi sayısının oviduktun her üç bölgesinde de luteal döneme göre daha yüksek olduğu gözlendi. Oviduktun diğer bölümlerine nazaran özellikle fimbriya bölgesinde, birim alandaki mast hücresi sayısının her iki dönemde de fazla olduğu görüldü (p<0.05). Elektron mikroskobik incelemelerde mast hücrelerinin; elektron açık, elektron yoğun ve ince tanecikli elektron açık granüller olmak üzere 3 tip granül içerdiği saptandı.Anahtar sözcükler: Domuz, elektron mikroskop, mast hücresi, ovidukt. Light and electron microscopic studies on mast cell of the sow oviductSummary: The present study was aimed to evaluate staining properties, light and electron microscopic structures and numerical distribution, of mast cells which are located at different regions of the swine oviduct. In the present study, oviduct samples taken from 6 healthy adult sows were used as material. It was observed that mast cells in regions of the oviduct gave Ab (Alcian blue) (+)/SO (Safranin O) (-) reaction. Results of the cell counting and their statistical analyses indicated that in oestrous phase the number of mast cells were significantly higher than luteal phase in the three parts of the oviduct. At both phases, mast cell numbers in the unit area were higher in fimbria region than those of the remaning regions of the oviduct (p<0.05). Results of electron microscopic observations revealed that mast cells contained three types of granulles those were electron lucent, electron dense and fine grain electron lucent granules.

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Nuclear estrogen and progesterone receptors in the oviduct of heifers under natural and superovulated estrous cycles

Cited by 24 publications

References 15 publications

Cellular Expression and Localization of Estrogen Receptor α and Progesterone Receptor mRNA in the Bovine Oviduct Combining Laser-Assisted Microdissection, Quantitative PCR, and In Situ Hybridization

Cellular Expression and Localization of Estrogen Receptor α and Progesterone Receptor mRNA in the Bovine Oviduct Combining Laser-Assisted Microdissection, Quantitative PCR, and In Situ Hybridization

MHC class II expression by mast cells in the genital tract of cows

Domuz oviduktunda mast hücreleri üzerinde ışık ve elektron mikroskobik çalışmalar

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