Nuclear DNA replication and seed quality

Śliwińska, Elwira

doi:10.1017/s0960258508186275

Cited by 36 publications

(31 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Different methods of priming usually activate the cell cycle in seeds by inducing DNA synthesis [for review see 30]. This event marks the transition to Phase II of germination which is the physiological stage of the seed preferable after the enhancement treatments .…”

Section: Discussionmentioning

confidence: 99%

Melatonin applied to cucumber (Cucumis sativus L.) seeds improves germination during chilling stress

Posmyk¹,

Bałabusta²,

Wieczorek³

et al. 2009

Journal of Pineal Research

235

183

View full text Add to dashboard Cite

The relationship between germination and melatonin applied during osmo- and hydropriming was studied in cucumber seeds. The proportion of nuclei with different DNA contents, the mean ploidy and the (2C + 4C = 8C)/2C ratio in unprimed and primed, dry and imbibed at 10 degrees C seeds were established by flow cytometry. Thiobarbituric acid reactive substances and protein oxidation were also estimated. Melatonin and indole-3-acetic acid (IAA) concentrations in the seeds were determined using high-performance liquid chromatography with electrochemical detection. Being sensitive to chilling stress, seeds that germinated well (99%) at 25 degrees C showed only 30% germination at 15 degrees C, and almost no germination (4%) at 10 degrees C. Hydropriming in water improved seed germination to 50-60% at 15 degrees C and the addition of melatonin (25-100 M) also increased the rate of germination. Osmopriming in polyethylene glycol increased germination at 15 degrees C to 78%, and 98% when combined with 50 M melatonin. Osmoprimed seeds germinated even at 10 degrees C and reached 43%, and 83% when 50 M melatonin was applied. None of the treatments induced DNA synthesis, although during the first 24 hr of imbibition at 10 degrees C the mean ploidy and the (2C + 4C = 8C)/2C ratio increased, which is indicative of the advanced Phase II of germination. Hydro- and osmopriming slightly decreased IAA content in the seeds in most of the cases; only hydropriming with 100 and 500 M melatonin increased it. Melatonin protected membrane structure against peroxidation during chilling, but excessive melatonin levels in cucumber seeds (approximately 4 microg/g fresh weight) provoked oxidative changes in proteins. There is still lack of information explained clearly the role of melatonin in plant physiology. This molecule acts multidirectionally and usually is alliged to other compounds.

show abstract

Section: Discussionmentioning

confidence: 99%

Melatonin applied to cucumber (Cucumis sativus L.) seeds improves germination during chilling stress

Posmyk¹,

Bałabusta²,

Wieczorek³

et al. 2009

Journal of Pineal Research

235

183

View full text Add to dashboard Cite

show abstract

“…In this kind of cell, the proportion of 4C/2C nuclei in the embryo, during the maturation and germination of the seeds, has been used as vigor mark (Sliwinska, 2009), since 2 C cells (G1 stage) are more resistant to stress and have greater longevity, when compared with 4 C cells (G2 stage). Besides, the state change from desiccation tolerant to sensitive in orthodox seeds coincides with the cells entrance in the G2 stage, which may be one of the causes of desiccation sensibility (Boubriak et al, 1997).…”

Section: Dna Contentmentioning

confidence: 99%

Physiological, cellular and molecular aspects of the desiccation tolerance in Anadenanthera colubrina seeds during germination

2017

View full text Add to dashboard Cite

During germination, orthodox seeds become gradually intolerant to desiccation, and for this reason, they are a good model for recalcitrance studies. In the present work, physiological, biochemical, and ultrastructural aspects of the desiccation tolerance were characterized during the germination process of Anadenanthera colubrina seeds. The seeds were imbibed during zero (control), 2, 8, 12 (no germinated seeds), and 18 hours (germinated seeds with 1 mm protruded radicle); then they were dried for 72 hours, rehydrated and evaluated for survivorship. Along the imbibition, cytometric and ultrastructural analysis were performed, besides the extraction of the heat-stable proteins. Posteriorly to imbibition and drying, the evaluation of ultrastructural damages was performed. Desiccation tolerance was fully lost after root protrusion. There was no increase in 4C DNA content after the loss of desiccation tolerance. Ultrastructural characteristics of cells from 1mm roots resembled those found in the recalcitrant seeds, in both hydrated and dehydrated states. The loss of desiccation tolerance coincided with the reduction of heat-stable proteins.Keywords: desiccation tolerance, forest seeds, drying.Aspectos fisiológicos, celulares e moleculares da tolerância à dessecação em sementes de Anadenanthera colubrina durante a germinação ResumoDurante a germinação, sementes ortodoxas tornam-se gradualmente intolerantes à dessecação, e por isso podem ser utilizadas como modelo para o estudo da recalcitrância. No presente trabalho realizou-se uma caracterização dos aspectos fisiológicos, bioquímicos e ultraestruturais da perda da tolerância à dessecação de sementes de Anadenanthera colubrina em processo germinativo. Para isso as sementes foram embebidas durante 0 (controle), 2,8,12 e aproximadamente 18 horas (sementes germinadas com 1 mm de radícula), secas por 72 horas, reidratadas e a sobrevivência avaliada. Ao longo da embebição foram realizadas análises citométricas, ultraestruturais e extração de proteínas resistentes ao calor e após embebição e secagem foram avaliados danos ultraestruturais. A tolerância à dessecação foi totalmente perdida após a protrusão radicular. Não houve aumento do conteúdo de DNA 4C quando a tolerância à dessecação foi perdida. Características ultraestruturais de células de radículas de 1 mm assemelharam-se às encontradas em sementes recalcitrantes tanto no estado hidratado quanto desidratado. A perda da tolerância à dessecação coincidiu com a redução do conteúdo de proteínas resistentes ao calor.Palavras-chave: tolerância à dessecação, sementes floretais, secagem.

show abstract

“…Among the techniques used to detect the changes that occur in the cells upon stress is the DNA electrophoresis (Faria et al 2005, Sliwinska 2009). The DNA cleavage into internucleosomal fragments and the DNA degradation in gel are accepted as a marker for programmed and passive cellular death in animal and plant cells, respectively (McCabe and Leaver 2000).…”

mentioning

confidence: 99%

Re-induction of desiccation tolerance after germination of Cedrela fissilis Vell. seeds

Masetto

Faria

Fraiz

2014

An. Acad. Bras. Ciênc.

View full text Add to dashboard Cite

This work aimed to characterize the re-induction of desiccation tolerance (DT) in germinated seeds, using polyethylene glycol (PEG 8000). Cell changes were investigated through cytological assays (cell viability and transmission electronic microscopy) as well as DNA integrity during loss and re-establishment of DT. The loss of DT was characterized by drying germinated seeds with different radicle lengths (1, 2, 3, 4 and 5 mm) in silica gel, decreasing the moisture content to ten percentage points intervals, followed by prehumidification (100% RH / 24 h) and rehydration. To re-induce DT, germinated seeds were treated for 72 h with PEG (-2.04 MPa) and PEG (-2.04 MPa) + ABA (100 μM) before dehydration. Germinated seeds did not tolerate desiccation to 10% moisture content, irrespectively of the radicle length. However, when incubated in PEG, those with 1 and 2 mm long radicle attained 71% and 29% survival, respectively. The PEG+ABA treatment was efficient to re-establish DT in seeds with 1 mm long radicles (100% survival). The ultrastructural assays of the cells of germinated seeds with 2 and 5 mm length confirmed the obtained physiological results. Germinated seeds of C. fissilis constitute a useful tool for desiccation tolerance investigations.

show abstract

Nuclear DNA replication and seed quality

Cited by 36 publications

References 67 publications

Melatonin applied to cucumber (Cucumis sativus L.) seeds improves germination during chilling stress

Melatonin applied to cucumber (Cucumis sativus L.) seeds improves germination during chilling stress

Physiological, cellular and molecular aspects of the desiccation tolerance in Anadenanthera colubrina seeds during germination

Re-induction of desiccation tolerance after germination of Cedrela fissilis Vell. seeds

Contact Info

Product

Resources

About