Nuclear DNA Microsatellites Reveal Genetic Variation but a Lack of Phylogeographical Structure in an Endangered Species, Fraxinus mandshurica, Across North-east China

Hu, Lijiang; Uchiyama, Kentaro; Shen, Hailong; Saito, Yoko; Tsuda, Yoshiaki; Ide, Yuji

doi:10.1093/aob/mcn074

Cited by 64 publications

(75 citation statements)

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“…In European studies of F. excelsior based on nSSR loci (Morand et al 2002;Heuertz et al 2001;FRAXIGEN 2005;Ferrazzini et al 2007), it was found that genetic diversity within populations and allelic richness were high. The same observation was reported for F. mandshurica in northeast China (Hu et al 2008). Moreover, two contrasting patterns of within and among population diversity between western and southern European populations of common ash have been observed (Heuertz et al 2004b;Ferrazzini et al 2007).…”

Section: Discussionsupporting

confidence: 81%

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Genetic variation of Fraxinus angustifolia natural populations in Greece based on nuclear and chloroplast microsatellite markers

2011

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Assessment of genetic variation within and among populations is an essential parameter for the effective conservation of forest genetic resources. In this work, the genetic diversity within and among natural populations of the forest tree species Fraxinus angustifolia Vahl in Greece was studied using selected nuclear and chloroplast microsatellite DNA loci. Eight natural populations of F. angustifolia were identified in different locations of the mainland of Greece, and a total of 230 individuals were studied. High polymorphism was observed within populations, while the genetic differentiation among populations was moderate. Intra-population diversity was correlated with geographical coordinates, but no isolation by distance was observed. Of the three haplotypes identified, only one was dominant. Putative ancestral haplotypes were found at small spatial scales suggesting that population expansions could have originated in the region. This study located in sympatry haplotypes that in other parts of Europe are in allopatry, reinforcing the notion of population expansions from the south of Balkans including Greece. Suggestions for conservation and management of F. angustifolia are also reported.

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Section: Discussionsupporting

confidence: 81%

“…Negative correlations of the genetic diversity with geographical gradients have been observed in F. mandshurica populations of NE China (Hu et al 2008). No evidence of correlation between geographical distance and genetic differentiation of the population studied was observed.…”

Section: Discussionmentioning

confidence: 73%

Genetic variation of Fraxinus angustifolia natural populations in Greece based on nuclear and chloroplast microsatellite markers

2011

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“…Similar results were also found for other wind-pollinated long-lived plants such as Fraxinus mandshurica Rupr. by Hu et al (2008).…”

Section: Phylogeography and Migration Routesmentioning

confidence: 93%

Genetic structure and phylogeography in Juniperus oxycedrus subsp. macrocarpa around the Mediterranean and Atlantic coasts of the Iberian Peninsula, based on AFLP and plastid markers

et al. 2011

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The Spanish populations of Juniperus oxycedrus subsp. macrocarpa are restricted to coastal dune habitats of the Mediterranean and Atlantic coasts. Here, we investigate the genetic structure in this taxon and the possible existence of detailed phylogeographic structure associated with the Strait of Gibraltar using data from amplified fragment length polymorphism (AFLP) and plastid markers for 14 populations in three regions: Andalusia, the Valencian Community and the Balearic Islands. Principal coordinate analysis (PCOA) data revealed a lack of clear genetic structure in J. oxycedrus subsp. macrocarpa based on AFLP. The highest AFLP genetic diversity was found in Andalusia. Plastid markers revealed 14 haplotypes, only two of them were shared among the regions. Andalusian populations had the highest number of exclusive haplotypes. Our results support Andalusia as a reservoir of genetic diversity for J. oxycedrus subsp. macrocarpa, and, as for other gymnosperms, this area could be considered as a genetic refugium. The frequent presence of common and exclusive haplotypes in Andalusia might indicate the colonisation of the Spanish Mediterranean coast from the Atlantic populations. Colonisation of these new areas could be promoted by biological factors, such as the existence of long-lived individuals or potential for seed dispersal. Finally, the Strait of Gibraltar did not constitute a natural barrier to the expansion of this species along the coast, as identical haplotypes are found on the Atlantic and Mediterranean coasts.

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“…for the argan forest based on alloenzymes (Fst=0.24) (El Mousadik and Petit, 1996) and based on ISSR markers (Gst= 0.40) (Mouhaddab et al, 2015) and (Gst= 0.39) (Yatrib et al, 2015). Compared to other deciduous woody plants with similar geographical distribution such as Fraxinus mandshrurica (He= 0.564; Fst= 0.010) (Hu et al, 2008), Acer mono (He= 0.737, Fst= 0.073) (Liu et al, 2014), the argan tree is considered as one of the most genetically differentiated forest resources. Hamrick et al, (1992) has reviewed plant alloenzymes literature in plant species, summarizing parameters of genetic diversity, with special attention to trees.…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity and population structure of the endangered argan tree (Argania spinosa L. Skeels) in morocco as revealed by SSR markers: Implication for conservation

Yatrib¹,

Belkadi²,

Medraoui³

et al. 2017

Aust J Crop Sci

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Argania spinosa L. is an endangered tree of great socio-economic and ecological value in Morocco. In this study, thirteen nuclear SSR primer pairs were used to assess the genetic diversity and structure of 24 natural populations, including 240 individuals, representing 4 geographic regions. A total of 245 alleles were detected with an average of 18.5 per locus ranging from 6 to 35. The polymorphism information content (PIC) was in the range of (0.487-0.936) showing the good discriminating power of the SSR loci used. The observed and the expected heterozygosity across all populations and loci ranged from 0.372 to 0.777 and from 0.486 to 0.735, respectively. Analysis of molecular variance (AMOVA) revealed that the main variation existed within populations (78%) rather than among populations (12%). The Mantel test displayed no significant correlation between the geographic distance and the genetic distances for all populations. The STRUCTURE analysis and UPGMA clustering grouped 240 samples from 24 populations into two subgroups. Implications of the results for argan tree conservations are also discussed in this paper.

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Nuclear DNA Microsatellites Reveal Genetic Variation but a Lack of Phylogeographical Structure in an Endangered Species, Fraxinus mandshurica, Across North-east China

Cited by 64 publications

References 50 publications

Genetic variation of Fraxinus angustifolia natural populations in Greece based on nuclear and chloroplast microsatellite markers

Genetic variation of Fraxinus angustifolia natural populations in Greece based on nuclear and chloroplast microsatellite markers

Genetic structure and phylogeography in Juniperus oxycedrus subsp. macrocarpa around the Mediterranean and Atlantic coasts of the Iberian Peninsula, based on AFLP and plastid markers

Genetic diversity and population structure of the endangered argan tree (Argania spinosa L. Skeels) in morocco as revealed by SSR markers: Implication for conservation

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