Nuclear DNA content of Pinus sylvestris (L.) as determined by laser flow cytometry

Valkonen, Jari P. T.; Nygren, Markku; Ylönen, Anne; Mannonen, Leena

doi:10.1007/bf00132539

Cited by 19 publications

(16 citation statements)

References 29 publications

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“…On the contrary, Hall et al (2000) detected variations in some tropical hard pines. Valkonen et al (1994) also showed some individual variations in Pinus sylvestris in different tissues, which would suggest a methodological source of variation.…”

Section: Introductionmentioning

confidence: 91%

“…and other gymnosperms have been mostly obtained by flow cytometry Wakamiya et al 1993;Valkonen et al 1994;O'Brien et al 1996;Murray 1998;Bou Dagher-Kharrat et al 2001;Siljak-Yakovlev et al 2002). Estimates obtained using this method have not shown such large discrepancies among populations for the same species and seem to be more accurate and reliable.…”

Section: Introductionmentioning

confidence: 97%

“…Pinus species have often been investigated for their genome size (Miksche 1968;Dhir and Miksche 1974;Teoh and Rees 1976;Greilhuber 1988;Wakamiya et al 1993;Valkonen et al 1994;O'Brien et al 1996;Murray 1998, Hall et al 2000Joyner et al 2001), but there are still species of this genus whose DNA values are unknown. Different opinions exist as to the reality or not of constancy in genome size.…”

Section: Introductionmentioning

confidence: 99%

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Genome size and base composition of five Pinus species from the Balkan region

et al. 2003

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The 2C DNA content and base composition of five Pinus (2 n=24) species and two Pinus subspecies from the Balkan region have been estimated by flow cytometry. P. heldreichii (five populations) and P. peuce (one population) were assessed for the first time, as also were subspecies of P. nigra (three populations-two of subspecies nigra and one of subspecies dalmatica) along with P. sylvestris, and P. mugo from the same region. The 2C DNA values of these Pinus ranged from 42.5 pg to 54.9 pg (41.7-53.8 x 10(9)bp), and the base composition was quite stable (about 39.5% GC). Significant differences were observed between two subspecies of P. nigra and even between two populations of subsp. nigra. The two other species (P. sylvestris and P. mugo) had 2C values of 42.5 pg and 42.8 pg, respectively, while that of P. peuce was 54.9 pg. These genome sizes are in accordance with published values except for P. sylvestris, which was 20% below estimates made by other authors.

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Section: Introductionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Genome size and base composition of five Pinus species from the Balkan region

et al. 2003

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“…All leaf samples (Ϸ50 mg each) were collected from the three-leaf stage. Protoplasts were isolated as before (34), then resuspended in 1 ml of nuclear buffer {30 mM sodium citrate, pH 7.0͞45 mM MgCl 2 ͞20 mM Mops [3(N-morpholino)propanesulfonic acid]͞1% (wt/vol) Triton X-100͞5 l(ml) Ϫ1 ␤-mercaptoethanol} (35,36), to which 20 m(ml)…”

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confidence: 99%

Genome evolution of wild barley ( Hordeum spontaneum ) by BARE -1 retrotransposon dynamics in response to sharp microclimatic divergence

Kalendar

Tanskanen

Immonen

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

542

393

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The replicative spread of retrotransposons in the genome creates new insertional polymorphisms, increasing retrotransposon numbers and potentially both their share of the genome and genome size. The BARE-1 retrotransposon constitutes a major, dispersed, active component of Hordeum genomes, and BARE-1 number is positively correlated with genome size. We have examined genome size and BARE-1 insertion patterns and number in wild barley, Hordeum spontaneum, in Evolution Canyon, Lower Nahal Oren, Mount Carmel, Israel, along a transect presenting sharply differing microclimates. BARE-1 has been sufficiently active for its insertional pattern to resolve individuals in a way consonant with their ecogeographical distribution in the canyon and to distinguish them from provenances outside the canyon. On both slopes, but especially on the drier south-facing slope, a simultaneous increase in the BARE-1 copy number and a decrease in the relative number lost through recombination, as measured by the abundance of solo long terminal repeats, appear to have driven the BARE-1 share of the genome upward with the height and dryness of the slope. The lower recombinational loss would favor maintenance of more full-length copies, enhancing the ability of the BARE-1 family to contribute to genome size growth. These local data are consistent with regional trends for BARE-1 in H. spontaneum across Israel and therefore may reflect adaptive selection for increasing genome size through retrotransposon activity.

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“…Recently employed techniques of fluorescent in situ hybridization (FISH) revealed organization of ribosomal genes in Pinus species [9,26,28,34]. Last decade was particularly marked by genome size investigations of Pinus species [2,20,29,39,40,48,50]. All these methods are very useful for consideration of phylogenetic and systematic relationships among pine species.…”

Section: Introductionmentioning

confidence: 99%

Chromosomal differentiation betweenPinus heldreichiiandPinus nigra

2006

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-Two tertiary relict pines, Pinus heldreichii and P. nigra, have been considered as taxonomically very close species. Both species have very discontinuous geographical distributions which overlap in some localities in Balkan Peninsula. The pattern of heterochromatin regions distribution (AT-, GC-rich and DAPI positive heterochromatin) and activity of nucleolar organizing regions (NORs) were analyzed for these two pines by fluorochrome banding and silver staining respectively. Morphometric data for karyotype of P. heldreichii were presented here for the first time. Chromomycin (CMA) banding pattern was particularly species specific, P. heldreichii possessed 12 and P. nigra 24 bands. In comparison with other species from subsection Pinus, P. heldreichii displayed particular CMA-banding pattern. Hoechst banding was less specific and similar to other pines. DAPI staining was applied after DNA denaturation/renaturation and revealed important differences in number and position of bands between the two species. Number of secondary constrictions (SCs), of NORs and of nucleoli also differed between P. heldreichii (10) and P. nigra (12). Our results proved a clear interspecific differentiation at the chromosomal level between the two species and add some data to discuss about the possible new subsectional placement of P. heldreichii. Pinus / heterochromatin / fluorochrome banding / G-C and A-T rich DNA / interspecific differentiationRésumé -Différentiation chromosomique entre Pinus heldreichii et Pinus nigra. Deux reliques tertiaires, Pinus heldreichii et P. nigra, sont considérées comme espèces taxonomiquement très proches. Leur distribution géographique est très discontinue, se chevauchant dans quelques localités des Balkans. La répartition des régions hétérochromatiques (AT-, GC-riche et hétérochromatine constitutive nonspécifique) et l'activité des organisateurs nucléolaires (NORs) sont analysées par fluorochrome banding et coloration au nitrate d'argent respectivement. Les données morphometriques du caryotype de P. heldreichii sont présentées ici pour la première fois. La distribution des bandes GC-riches est particulièrement spécifique, P. heldreichii possède 12 et P. nigra 24 bandes. En comparaison avec d'autres espèces de la subsection Pinus, P. heldreichii présente un chromomycin banding particulier. Les bandes AT-riches sont moins spécifiques et leur localisation est similaire aux autres pins. La coloration au DAPI, effectuée après la dénaturation/renaturation de l'ADN, révèle des différences importantes en nombre et position des bandes entre deux espèces. Le nombre des constrictions secondaires (SCs), des NORs et des nucléoles diffère également entre P. heldreichii (10) et P. nigra (12). Nos résultats montrent une différentiation nette au niveau chromosomique entre ces deux espèces et ouvrent la discussion sur un changement éventuel de subsection pour P. heldreichii. Pinus / hétérochromatine / fluorochrome banding / ADN riche en G-C et A-T / différentiation interspécifique

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Nuclear DNA content of Pinus sylvestris (L.) as determined by laser flow cytometry

Cited by 19 publications

References 29 publications

Genome size and base composition of five Pinus species from the Balkan region

Genome size and base composition of five Pinus species from the Balkan region

Genome evolution of wild barley ( Hordeum spontaneum ) by BARE -1 retrotransposon dynamics in response to sharp microclimatic divergence

Chromosomal differentiation betweenPinus heldreichiiandPinus nigra

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