Nuclear DNA Content of Myxamoebae and Plasmodia in Six Non-Heterothallic Isolates of a Myxomycete, Didymium iridis

Therrien, C. D.; Bell, W. R.; Collins, O’Neil Ray

doi:10.2307/2441972

Cited by 8 publications

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“…show that in Didymium iridis apogamic lines can be developed from heterothallic ones. These results may help to explain the evolutionary relationships between reproductive systems in the Myxomycetes, especially in D. iridis, a taxon whose naturally occurring individual members may be either heterothallic, homothallic or apomictic (Therrien, Bell, and Collins, 1977). The new apogamic lines, along with those known in P. polycephalum (Adler and Holt, 1975) also provide useful material for the study of the function of the mating locus itself, as well as for future investigations on myxomycete speciation.…”

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confidence: 80%

Development of Apogamic Amoebae From Heterothallic Lines of a Myxomycete, Didymium Iridis

Mulleavy

Collins

1979

American J of Botany

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By making appropriate crosses between heterothallic sexual clones of Didymium iridis we can recover apogamic lines in the F1 generation. In this organism, heterothallic forms typically produce a haploid myxamoebal stage, but recently two diploid myxamoebal clones homozygous for mating types were discovered. When these are crossed, A2A2 x A5A5, tetraploid Plasmodia are produced which later yield diploid F1 meiospores. Sixty‐four percent of the single‐spore‐derived clones produce both myxamoebae and Plasmodia, while the remainder do not progress past the myxamoebal stage. These results are consistent with the predictions that from tetraploid nuclei, mating types should segregate in the meiospores in a ratio of 1A2A2:4A2A5:1A5A5, and that myxamoebae heterozygous, A2A5, for mating type should yield Plasmodia apogamously. As the means for verifying relative ploidy levels of myxamoebae and Plasmodia, nuclear DNA was measured with a scanning microspectrophotometer.

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confidence: 80%

Development of Apogamic Amoebae From Heterothallic Lines of a Myxomycete, Didymium Iridis

Mulleavy

Collins

1979

American J of Botany

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“…Such apomictic strains could be haploid or diploid during the entire cycle without any change in the nuclear content and, if diploid, could be homozygous or heterozygous, depending on the state of the sexual ancestors ( Table 1 ). Whether asexual lineages are truly apomictic or have a cryptic sexuality is still unclear, but some lines of evidence point towards true apomixis: isolates usually do not crossbreed [27] and measurements of DNA contents in myxamoebae and plasmodia are identical and appear to be 2n [30] . Cryptic sexuality may thus be only a hypothetical option that could not be ruled out by culture-based studies.…”

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confidence: 99%

Genetic Structure of Two Protist Species (Myxogastria, Amoebozoa) Suggests Asexual Reproduction in Sexual Amoebae

Fiore-Donno

Novozhilov²,

Meyer³

et al. 2011

PLoS ONE

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Plasmodial slime molds (Myxogastria or Myxomycetes) are common and widespread unicellular organisms that are commonly assumed to have a sexual life cycle culminating with the formation of often macroscopic fruiting bodies that efficiently disseminate spores. However, laboratory studies based on mating compatibility revealed the coexistence of asexual as well as sexual strains. To test this hypothesis in natural populations, we investigated the genetic variability of two species of the genus Lamproderma. Detailed ecological relevés were carried out in 2007 and 2009 in several deep ravines in the Elbsandsteingebirge (Saxony, south-eastern Germany). Morphological characters of 93 specimens of Lamproderma were recorded and genetic analyses, based on the small subunit ribosomal gene, the internal transcribed spacer 1 and partial elongation factor 1α sequences were carried out for 52 specimens. Genetic analyses showed the existence of two major clades, each composed of several discrete lineages. Most of these lineages were composed of several identical sequences (SSU, ITS 1 and EF-1α) which is explained best by an asexual mode of reproduction. Detrended Correspondence Analysis of morphological characters revealed two morphospecies that corresponded to the two major clades, except for one genotype (Lc6), thus challenging the morphospecies concept. Genetic patterns were not related to the geographical distribution: specimens belonging to the same genotype were found in distinct ravines, suggesting effective long-distance dispersal via spores, except for the Lc6 genotype which was found only in one ravine. Implications for the morphological and biological species concept are discussed.

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Cellular Interaction in Plasmodial Slime Moulds

Schrauwen¹

1984

Cellular Interactions

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Nuclear DNA Content of Myxamoebae and Plasmodia in Six Non-Heterothallic Isolates of a Myxomycete, Didymium iridis

Cited by 8 publications

References 0 publications

Development of Apogamic Amoebae From Heterothallic Lines of a Myxomycete, Didymium Iridis

Development of Apogamic Amoebae From Heterothallic Lines of a Myxomycete, Didymium Iridis

Genetic Structure of Two Protist Species (Myxogastria, Amoebozoa) Suggests Asexual Reproduction in Sexual Amoebae

Cellular Interaction in Plasmodial Slime Moulds

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