Nuclear DNA Content as an Index Character Discriminating Taxa in the Genus Petunia sensu Jussieu (Solanaceae)

Mishiba, Kei‐ichiro; Ando, Toshio; Mii, Masahiro; Watanabe, Hitoshi; Kokubun, Hisashi; Hashimoto, Goro; Marchesi, Eduardo

doi:10.1006/anbo.2000.1122

Cited by 83 publications

(45 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After filtration through a 30-μm nylon mesh, crude nuclear samples were stained with 0.8 ml DAPI solution containing 10 mM Tris, 50 mM sodium citrate, 2 mM MgCl 2 , 1% (w/v) PVP K-30, 0.1% (v/v) Triton X-100, and 2 mg l -1 DAPI (pH 7.5) (Mishiba et al 2000 Relative DNA content was estimated by the ratio between the fluorescence intensity of the sample and internal standard: sample relative DNA content = sample peak mean / standard peak mean. The relative DNA content was then calculated for each sample as the average of the three replications.…”

Section: Plant Materialsmentioning

confidence: 99%

Ploidy distribution and DNA content variations of Lonicera caerulea (Caprifoliaceae) in Japan

2010

View full text Add to dashboard Cite

Ploidy level and geographical distribution were investigated in Japanese Lonicera caerulea L. Flow cytometric analysis revealed the presence of DNA diploid and DNA tetraploid plants in Japan. Chromosome observation confirmed that diploid and tetraploid plants showed 2n = 2x = 18 and 2n = 4x = 36, respectively. The DNA diploid populations were found only in lowland mires, Betsukai, Bekanbeushi, Kushiro and Kiritappu located in eastern Hokkaido. On the other hand, DNA tetraploid populations were distributed in a wide area of Hokkaido, and mainland of Japan. The habitats of DNA tetraploid plants were lowland to alpine region. The DNA content measurement with flow cytometry revealed significant differences in the relative DNA contents among DNA tetraploid populations. The relative DNA content within DNA tetraploid populations varied 1.157-fold at maximum, and might correlate with altitude indicating that DNA contents were smaller as altitude increases. The wide area of distribution in various environments of DNA tetraploid plants suggested the adaptability of the tetraploid plants. Although diploid and tetraploid populations were found, no triploid was detected, indicating crossing difficulty between diploid and tetraploid as confirmed by crossing experiment.

show abstract

Section: Plant Materialsmentioning

confidence: 99%

Ploidy distribution and DNA content variations of Lonicera caerulea (Caprifoliaceae) in Japan

2010

View full text Add to dashboard Cite

show abstract

“…After filtration through a 30 μm nylon mesh, crude nuclear samples were stained with 0.7 mL of DAPI (4',6-diamidino-2-phenylindole) solution containing 10 mM Tris, 50 mM sodium citrate, 2 mM MgCl 2 , 1% (w/v) polyvinylpyrrolidone (PVP) K-30, 0.1% (v/v) Triton X-100, and 2 mg·L −1 DAPI (pH 7.5) (Mishiba et al, 2000). After incubation for 30 s at room temperature, nuclear fluorescence was measured using a ploidy analyzer (Partec PA; Partec).…”

Section: ) Flow Cytometric Analysismentioning

confidence: 99%

Distribution, Ploidy Levels, and Fruit Characteristics of Three <i>Actinidia</i> Species Native to Hokkaido, Japan

Asakura

Hoshino

2016

Hortic J

View full text Add to dashboard Cite

The genus Actinidia includes widely-sold kiwifruit, and is thus horticulturally important. We investigated the distribution, ploidy levels, and fruit characteristics of the natural populations of three edible Actinidia species [Actinidia arguta (Siebold & Zucc.) Planch. ex Miq., Actinidia kolomikta (Maxim. & Rupr.) Maxim., and Actinidia polygama (Siebold & Zucc.) Planch. ex Maxim.] in Hokkaido, the northern island of Japan. Actinidia arguta and A. kolomikta were common, and their habitat ranges overlapped. Actinidia polygama was less common, and its habitat was mostly limited to lowland deciduous forests. Flow cytometric analysis revealed that all wild collections of A. kolomikta and A. polygama were diploid, and that A. arguta was tetraploid, suggesting a lack of intraspecific ploidy variation. Fruit shape varied from round to ovoid in A. arguta, ranged from ovoid to ellipsoidal in A. kolomikta, and was ellipsoidal in A. polygama. The fruit skin of all species was glabrous, and skin color was orange in A. polygama, green to dark green in A. kolomikta, and light to dark green in A. arguta. The fresh weight of A. kolomikta fruit was less than that of A. arguta, and the soluble solids content (SSC) of the fruits varied widely within species. One sample of A. arguta had extremely high SSC (average Brix of 30.8%). The ascorbic acid content (AAC) was the highest in A. kolomikta (up to 805 mg per 100 g fresh weight). Actinidia arguta and A. kolomikta germplasm may be useful for breeding new kiwifruit varieties for cultivation in cold-temperate regions.

show abstract

“…To induce a larger-flowered phenotype of petunia, it is desirable to identify G and elucidate the underlying molecular mechanism of flower enlargement and of these pleiotropic effects. For this purpose, a genetic approach using a linkage map (Bossolini et al, 2011) might be effective, although large genome size (1200-1500 Mbp, Mishiba et al, 2000) may be a barrier to the identification of G. It may be useful to perform transcriptome analysis between gg and Gg plants based on ample EST data deposited in public databases such as the DNA Data Bank of Japan (DDBJ, http://www.ddbj.nig.ac.jp/) to specify gene regulation networks influenced by G.…”

Section: ) Organ-dependent Effect Of the G Allelementioning

confidence: 99%

Large Flower Size: Molecular Basis and Role of Cytokinin

Nishijima

2012

J. Japan. Soc. Hort. Sci.

View full text Add to dashboard Cite

Large flower size is an important trait that influences the economic value of floricultural plants. Large flower size is conferred by two morphological changes i.e. increased petal number and individual petal size. Increased petal number is induced by enlargement of the floral meristem or homeotic conversion of stamens and carpels into petals. Genes regulating meristematic competence mediate enlargement of the floral meristem, while floral homeotic genes mediate the homeotic conversion of floral organs. Increased individual petal size is induced by increased cell size or cell number. Polyploidization of the nuclear genome increases petal cell size, while increased petal cell number is induced by enhanced expression of the genes involved in auxin signal transduction and cytokinin biosynthesis. In this review, the molecular mechanisms affecting flower size, elucidated mainly using model plants such as Arabidopsis, are summarized. Further, our research on the roles of cytokinin biosynthesis and signal transduction in increased flower size in petunia, induced by the single major gene Grandiflora, is discussed. Based on these results, a possible systematic breeding method for increasing flower size is discussed.

show abstract

Nuclear DNA Content as an Index Character Discriminating Taxa in the Genus Petunia sensu Jussieu (Solanaceae)

Cited by 83 publications

References 30 publications

Ploidy distribution and DNA content variations of Lonicera caerulea (Caprifoliaceae) in Japan

Ploidy distribution and DNA content variations of Lonicera caerulea (Caprifoliaceae) in Japan

Distribution, Ploidy Levels, and Fruit Characteristics of Three <i>Actinidia</i> Species Native to Hokkaido, Japan

Large Flower Size: Molecular Basis and Role of Cytokinin

Contact Info

Product

Resources

About