Nuclear DNA constancy: A critical evaluation of some exceptions reported by lison and pasteels

Alfert, Max; Swift, Hewson

doi:10.1016/0014-4827(53)90232-8

Cited by 45 publications

(3 citation statements)

References 8 publications

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“…Protein interference in Feulgen reaction has also been referred to by some authors (1,6,21,25). They observed that the loss of protein in nuclei leads to higher Feulgen intensity.…”

Section: Discussionmentioning

confidence: 78%

Optimal Feulgen Hydrolysis for Microspectrophotometry

Tomonaga

Nomura

Awaya

1971

Acta Histochem. Cytochem

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“…Protein interference in Feulgen reaction has also been referred to by some authors (1,6,21,25). They observed that the loss of protein in nuclei leads to higher Feulgen intensity.…”

Section: Discussionmentioning

confidence: 78%

Optimal Feulgen Hydrolysis for Microspectrophotometry

Tomonaga

Nomura

Awaya

1971

Acta Histochem. Cytochem

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“…It has recently been reported that colchicine is not directly concerned with DNA synthesis in spite of blocking mitosis at the metaphase, (see the work of Bloch on the occurrence of polyploidy in the colchicine-treated fibroblast (4)). Since it has also been reported in various experiments that the synthesis of DNA takes place in the interphase period (1,12,19,62,64,69), it may be assumed that most of the liver cells in adult rats synthesize new molecules of DNA in the interphase period, and thereafter keep themselves continuously in the interphase condition without the formation of visible chromosomes. This consideration leads to the idea that the mechanism of the DNA synthesis is not directly linked to the mitotic mechanism.…”

Section: (15)mentioning

confidence: 99%

Microspectrophotometry of Cell Nuclei Stained With the Feulgen Reaction

Naora

1957

The Journal of Cell Biology

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1. DNA contents of the individual parenchymal nuclei of rat livers during postnatal growth were estimated by microspectrophotometric apparatus, and different ploidy classes of nuclei were classified by their DNA contents. With the same material the total number of parenchymal nuclei in the liver was counted microscopically. 2. If the DNA content of nuclei encountered most frequently in several tissues represents the diploid class, the ploidy classes of the rat liver cell nuclei correspond to di-, tri-, tetra-, and octoploid, with the di- and tetraploid ones predominating considerably. 3. In suckling rats (below 25 gm. of body weight) the liver parenchyma is composed almost exclusively of cells with diploid nuclei, whereas in young rats (above 80 gm.), of tetraploid nuclei. In the growth stage between 25 and 80 gm., there is a remarkable replacement of the diploid nuclei by the tetraploid ones. However, in the liver of adult rats weighing more than 150 gm., any increase or decrease in the frequency of diploid and tetraploid nuclei is hardly observable. In such rats, the nuclear population of the liver parenchyma seems to reach a cell-ecological equilibrium which is considered to be a stable one. 4. It is shown that such nuclear populations and the total number of nuclei in a liver are controlled by the growth state, and not by the age. 5. The decrease in the total number of diploid nuclei and the increase in tetraploid nuclei in the growing livers of rats weighing from 40 up to 130 gm. can both be explained by the hypothesis that the tetraploid nuclei originate from the interphase diploid nuclei without involving mitosis. This hypothesis implies that mitosis is confined to the reproduction of diploid cells alone. 6. It is suggested that, in general, the synthesis of DNA does not necessarily result in the formation of visible mitotic chromosomes. 7. Mitotic time and generation time of diploid nuclei and the percentage of the tetraploidization from diploid nuclei are calculated and discussed.

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“…In the previous experiments, the amount of thymine per egg, when determined as DNA, was found to be equivalent to 10X in which X was the amotmt per sperm. From experiments on other materials which indicated that the polar bodies each had the X amount of DNA (13,14), it was assumed that 3X of the total could be reasonably assigned to the polar bodies. In addition it was found that in spite of repeated washings there was a considerable number of somatic cells in the preparation (1).…”

Section: Discussionmentioning

confidence: 99%

Thymine in the Acid-Soluble Fraction of Arbacia Eggs

Marshak

1955

The Journal of Cell Biology

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Mature Arbacia eggs were extracted with cold dilute perchloric acid, the extract concentrated, and the concentrate digested in hot perchloric acid. Thymine was recovered from the digest by paper chromatography, and the amount per egg found to be about 5 times the amount per sperm. This was the amount expected from previous experiments and is believed to represent all or almost all of the thymine in the egg. The result supports previous observations that DNA is absent from the mature egg although present in the nucleus of the egg in the germinal vesicle stage. No thymine could be recovered from a similar extract of 5,000 times as many sperm of the same species. The observations are consistent with the theory that DNA and its derivatives act as metabolic antagonists of the corresponding ribose compounds.

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Nuclear DNA constancy: A critical evaluation of some exceptions reported by lison and pasteels

Cited by 45 publications

References 8 publications

Optimal Feulgen Hydrolysis for Microspectrophotometry

Optimal Feulgen Hydrolysis for Microspectrophotometry

Microspectrophotometry of Cell Nuclei Stained With the Feulgen Reaction

Thymine in the Acid-Soluble Fraction of Arbacia Eggs

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