Nuclear cloning of embryonal carcinoma cells

Blelloch, Robert; Hochedlinger, Konrad; Yamada, Yasuhiro; Brennan, Cameron; Kim, Minjung; Mintz, Beatrice; Chin, Lynda; Jaenisch, Rudolf

doi:10.1073/pnas.0405015101

Cited by 92 publications

(105 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Pioneering studies that investigated the plasticity of the cancer cell genome used nuclear cloning experiments (29,30). These studies showed that some cancer nuclei are competent for the initial developmental stage after nuclear transplantation.…”

Section: Discussionmentioning

confidence: 99%

Cellular context-dependent consequences of Apc mutations on gene regulation and cellular behavior

Hashimoto

Yamada

Semi

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

The spectrum of genetic mutations differs among cancers in different organs, implying a cellular context-dependent effect for genetic aberrations. However, the extent to which the cellular context affects the consequences of oncogenic mutations remains to be fully elucidated. We reprogrammed colon tumor cells in an Apc Min/+ (adenomatous polyposis coli) mouse model, in which the loss of the Apc gene plays a critical role in tumor development and subsequently, established reprogrammed tumor cells (RTCs) that exhibit pluripotent stem cell (PSC)-like signatures of gene expression. We show that the majority of the genes in RTCs that were affected by Apc mutations did not overlap with the genes affected in the intestine. RTCs lacked pluripotency but exhibited an increased expression of Cdx2 and a differentiation propensity that was biased toward the trophectoderm cell lineage. Genetic rescue of the mutated Apc allele conferred pluripotency on RTCs and enabled their differentiation into various cell types in vivo. The redisruption of Apc in RTC-derived differentiated cells resulted in neoplastic growth that was exclusive to the intestine, but the majority of the intestinal lesions remained as pretumoral microadenomas. These results highlight the significant influence of cellular context on gene regulation, cellular plasticity, and cellular behavior in response to the loss of the Apc function. Our results also imply that the transition from microadenomas to macroscopic tumors is reprogrammable, which underscores the importance of epigenetic regulation on tumor promotion. iPS cell | cancer epigenetics | plasticity | colon cancer | mouse model

show abstract

Section: Discussionmentioning

confidence: 99%

Cellular context-dependent consequences of Apc mutations on gene regulation and cellular behavior

Hashimoto

Yamada

Semi

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…To determine whether other pluripotent stem cell lines differ in developmental potential, we next asked whether ECCs could give rise to TSCs. ECCs can contribute to fetal development in chimeras [38][39][40][41], suggesting that ECCs and ESCs are comparable in terms of developmental potential. To determine whether ECCs can also give rise to TSCs following Cdx2 overexpression, we introduced the Cdx2ER expression plasmid, selected subclones expressing appropriate levels of Cdx2ER ( Supplementary Fig.…”

Section: Eccs Generate Cells With Tsc Properties Following Cdx2 Overementioning

confidence: 99%

Cdx2Efficiently Induces Trophoblast Stem-Like Cells in Naïve, but Not Primed, Pluripotent Stem Cells

Blij

Parenti

Tabatabai-Yazdi

et al. 2015

Stem Cells and Development

View full text Add to dashboard Cite

“…First, the epigenetic modifications in somatic genome can affect the efficiency of SCNT. For example, in the mouse model, compared with terminally differentiated somatic cells, both ESCs and neural stem cells were easier to be reprogrammed by oocytes (Blelloch et al 2004(Blelloch et al , 2006. Second, in cloned embryos, the abnormalities in overall epigenetic events just make a possibility for the occurrence of some epigenetic events, which intrinsically cannot occur in fertilized embryos.…”

Section: Resultsmentioning

confidence: 99%

Somatic cell reprogrammed by oocyte: process and barricade

Wang

et al. 2014

Animal Cells and Systems

View full text Add to dashboard Cite

Somatic cell nuclear transfer (SCNT) is a technology in which a somatic nucleus is transferred into the cytoplasm of a matured oocyte -reconstructed embryos have the capacity to develop to term. Why somatic cells can be reprogrammed by oocytes -the answer for this question must exist in the cytoplasm of matured oocytes. In this review, totipotent characters of matured oocytes were discussed, which may confer matured oocytes with the capacity to reprogram somatic nucleus. Moreover, the procedure of SCNT also makes a possibility for somatic nucleus to be reprogrammed by oocytes. Compared with fertilized embryos, embryos derived from SCNT exhibit low developmental ability; the barricades in reprogramming process and their possible reasons were also discussed. This review maybe can benefit the mechanism research of SCNT technology and can make contribution for improving the efficiency of this technology.

show abstract

Nuclear cloning of embryonal carcinoma cells

Cited by 92 publications

References 34 publications

Cellular context-dependent consequences of Apc mutations on gene regulation and cellular behavior

Cellular context-dependent consequences of Apc mutations on gene regulation and cellular behavior

Cdx2Efficiently Induces Trophoblast Stem-Like Cells in Naïve, but Not Primed, Pluripotent Stem Cells

Somatic cell reprogrammed by oocyte: process and barricade

Contact Info

Product

Resources

About