Nuclear bodies in the oocyte nucleus of ground beetles are enriched in snRNPs

Jaglarz, Mariusz K.

doi:10.1054/tice.2001.0193

Cited by 20 publications

(19 citation statements)

References 26 publications

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“…It has recently been speculated that the Cajal bodies in amphibian and insect oocytes represent storage structures that provide the snRNPs needed for early stages of embryogenesis (Gall 1991;Parfenov et al 1996;Jaglarz 2001). This hypothesis is supported by studies showing that Xenopus oocytes contain enough stored U1 and U2 snRNPs to supply all blastomere nuclei up to the midblastula stage (Forbes et al 1983), and the observation that in amphibians Cajal bodies are still present in mature oocytes in which transcription (and consequently splicing) has already been terminated (Gall 1991;Parfenov et al 1996).…”

Section: Discussionmentioning

confidence: 96%

“…Cajal bodies found in amphibian and insect oocytes are exceptionally large and often numerous (Gall 1991;Gall et al 1995Gall et al , 1999Bogolyubov et al 2000;Jaglarz 2001). It is believed that in female gametes the snRNPs accumulated in Cajal bodies (termed also nuclear bodies or endobodies) are not only used for the processing of nascent transcripts but serve as a source of processing factors for the future embryo (Gall 1991;Parfenov et al 1996;Bogolyubov et al 2000;Jaglarz 2001). Cajal bodies from Xenopus oocytes consist of a spherical matrix and associated B snurposomes that are composed of dense 20-30 nm particles -the transcriptosomes Gall 2000).…”

Section: Introductionmentioning

confidence: 99%

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Accessory nuclei revisited: the translocation of snRNPs from the germinal vesicle to the periphery of the future embryo

Biliński

Kloc

2002

Chromosoma

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Oocytes of certain insects contain peculiar organelles termed accessory nuclei (AN). These organelles originate by budding off from the envelope of the oocyte nucleus and contain 1-2 dense inclusions immersed in a translucent ground substance. We have demonstrated that in the wasp Vespula germanica each inclusion consists of two elements: a spherical body, and a hemispherical structure composed of numerous 20-30 nm particles. Immunoelectron microscopy and whole-mount in situ hybridization have shown that the inclusions contain AgNOR-staining proteins, p80-coilin, Sm proteins, and small nuclear RNAs (snRNAs). These results indicate that the inclusions and hemispherical structures are homologous to Cajal bodies and B-snurposomes of Xenopus germinal vesicles, respectively. During previtellogenesis, AN (together with their Cajal bodies) migrate to the cortical ooplasm of the oocyte where they reside at least until the onset of embryogenesis. We suggest that AN are vehicles for the transport and localization of snRNPs to the periphery of the oocyte, i.e., to the region where the blastoderm of the embryo develops and where there is a requirement for a high concentration of RNA-processing factors.

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Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Accessory nuclei revisited: the translocation of snRNPs from the germinal vesicle to the periphery of the future embryo

Biliński

Kloc

2002

Chromosoma

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“…They have been described in oocytes of different insects including the house cricket and mole cricket (Jörgensen 1913;Gall et al 1995;Tsvetkov et al 1997;Filek et al 2002), a dragonfly (Tsvetkov et al 1996), some beetles (Bogolyubov and Parfenov 2001;Jaglarz 2001), and the common wasp (Jabłońska and Biliński 2001;Biliński and Kloc 2002). However, no NBs containing snRNPs, SC35 protein and/or coilin were identified by immunoelectron microscopy in oocytes of the apple blossom weevil (Świątek and Jaglarz 2004).…”

Section: Discussionmentioning

confidence: 99%

Identification and dynamics of Cajal bodies in relation to karyosphere formation in scorpionfly oocytes

et al. 2005

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In oocyte nuclei of the scorpionfly, Panorpa communis, we have recently defined a population of nuclear bodies (NBs) that contain some components of Cajal bodies (CBs). In the present study, we used several criteria [presence of coilin, U7 snRNA, RNA polymerase II (pol II) and specific ultrastructure] to identify these NBs as CBs. The essential evidence for CB identification came from experiments with microinjection of fluorescein-tagged U7 snRNA. Consistent with the U7 data, we found pol II and pre-mRNA splicing factor, SC35, in Panorpa oocyte CBs. We show here that the dynamics of CBs differs from that in somatic cells and correlates with the level of oocyte chromosome condensation. We also found that the significant increase of CB size is accompanied by condensation of the chromosomes in the karyosphere, which is indicative of a decline in transcription. Using immunogold microscopy we determined that pol II and coilin are shared by CBs and the granular material associated with condensed chromosomes in the Panorpa karyosphere. The colocalization of pol II, U7 snRNA and splicing factors with CBs at the inactive stage of late oogenesis suggests that the latter may serve as storage domains for components that were earlier engaged in RNA transcription and processing.

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“…The nature of some characteristic inclusions observed in oocytes of different insects as the CBs are established now [2][3][4][5] whereas until recently little is known about insect oocyte IGC structure and functions. Insect oocyte IGCs have been described only in a beetle [6,7], the house cricket [8], a fleshfly [9] and some bugs [10].…”

Section: Introductionmentioning

confidence: 99%

Factors related to RNA polymerase II transcription are localized in interchromatin granule clusters of Panorpa communis oocytes.

Batalova

Parfenov²

2009

Folia Histochem Cytobiol.

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Abstract. Diplotene oocyte nucleus of the scorpionfly Panorpa communis is transcriptionally silent and contains numerous nuclear bodies including interchromatin granule clusters (IGCs). The latter consist of the granules of 30-50 nm in diameter and contain IGC marker protein SC35 as well as RNA polymerase II. In this study, we also localized in P. communis oocyte IGCs the transcription coactivators CBP/p300, TATA-binding protein (TBP) which is a component of the basal transcription factor TFIID and the basal transcription factor TFIIH. We belive that IGCs in transcriptionally inert P. communis oocytes are storage sites for the components of RNA polymerase II holoenzyme and other factors of RNA pol II transcription.

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Nuclear bodies in the oocyte nucleus of ground beetles are enriched in snRNPs

Cited by 20 publications

References 26 publications

Accessory nuclei revisited: the translocation of snRNPs from the germinal vesicle to the periphery of the future embryo

Accessory nuclei revisited: the translocation of snRNPs from the germinal vesicle to the periphery of the future embryo

Identification and dynamics of Cajal bodies in relation to karyosphere formation in scorpionfly oocytes

Factors related to RNA polymerase II transcription are localized in interchromatin granule clusters of Panorpa communis oocytes.

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