Nuclear Architecture and Chromatin Dynamics in Interphase Nuclei of &lt;b&gt;&lt;i&gt;Arabidopsis thaliana&lt;/i&gt;&lt;/b&gt;

Prete, Stefania Del; Arpòn, Javier; Sakai, Kaori; Andrey, Philippe; Gaudin, Valérie

doi:10.1159/000363724

Cited by 21 publications

(15 citation statements)

References 215 publications

(291 reference statements)

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“…Collectively, these cytogenetic and molecular studies support the view that Arabidopsis nuclear genome organization follows geometrical and biophysical constraints but is otherwise random (Del Prete et al 2014;Schubert et al 2012), at least in somatic tissues.…”

Section: Introductionsupporting

confidence: 62%

Non-random chromosome arrangement in triploid endosperm nuclei

et al. 2016

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The endosperm is at the center of successful seed formation in flowering plants. Being itself a product of fertilization, it is devoted to nourish the developing embryo and typically possesses a triploid genome consisting of two maternal and one paternal genome complement. Interestingly, endosperm development is controlled by epigenetic mechanisms conferring parent-of-origin-dependent effects that influence seed development. In the model plant Arabidopsis thaliana, we have previously described an endosperm-specific heterochromatin fraction, which increases with higher maternal, but not paternal, genome dosage. Here, we report a detailed analysis of chromosomal arrangement and association frequency in endosperm nuclei. We found that centromeric FISH signals in isolated nuclei show a planar alignment that may results from a semi-rigid, connective structure between chromosomes. Importantly, we found frequent pairwise association of centromeres, chromosomal segments, and entire arms of chromosomes in 3C endosperm nuclei. These associations deviate from random expectations predicted by numerical simulations. Therefore, we suggest a non-random chromosomal organization in the triploid nuclei of Arabidopsis endosperm. This contrasts with the prevailing random arrangement of chromosome territories in somatic nuclei. Based on observations on a series of nuclei with varying parental genome ratios, we propose a model where chromosomes associate pairwise involving one maternal and one paternal complement. The functional implications of this predicted chromosomal arrangement are discussed.

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Section: Introductionsupporting

confidence: 62%

Non-random chromosome arrangement in triploid endosperm nuclei

et al. 2016

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“…Also the chromatin fibers inside keep mainly their position. But in addition, other vesicular structures (possibly nuclear bodies containing components involved in splicing, transcription or gene silencing; Del Prete et al, 2014) appear (Figure 6; Suppl. movie 1).…”

Section: Resultsmentioning

confidence: 99%

Chromatin associations in Arabidopsis interphase nuclei

2014

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The arrangement of chromatin within interphase nuclei seems to be caused by topological constraints and related to gene expression depending on tissue and developmental stage. In yeast and animals it was found that homologous and heterologous chromatin association are required to realize faithful expression and DNA repair. To test whether such associations are present in plants we analyzed Arabidopsis thaliana interphase nuclei by FISH using probes from different chromosomes. We found that chromatin fiber movement and variable associations, although in general relatively seldom, may occur between euchromatin segments along chromosomes, sometimes even over large distances. The combination of euchromatin segments bearing high or low co-expressing genes did not reveal different association frequencies probably due to adjacent genes of deviating expression patterns. Based on previous data and on FISH analyses presented here, we conclude that the global interphase chromatin organization in A. thaliana is relatively stable, due to the location of its 10 centromeres at the nuclear periphery and of the telomeres mainly at the centrally localized nucleolus. Nevertheless, chromatin movement enables a flexible spatial genome arrangement in plant nuclei.

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“…Plant nuclei differ from those in animal cells by components of the nuclear envelope [9][10][11] and exhibit a diverse and highly dynamic organization of their chromosome and chromatin content [recent reviews by [12][13][14][15]. Characteristic for several plant species is the tendency of heterochromatin to form clusters that appear condensed even in interphase.…”

Section: Organization Of Plant Nucleimentioning

confidence: 99%

“…The number, organization, and position of chromocenters in Arabidopsis nuclei varies with ploidy [20], cell type [12,21], between accessions [22], and can be affected in plants lacking chromatin modifiers or remodelers [23,24]. Furthermore, specific developmental phase transitions, which require important changes in gene expression, are accompanied by transient alterations of chromocenter organization [17].…”

Section: Changes In Chromocenters During Development and Stressmentioning

confidence: 99%

Stress-induced structural changes in plant chromatin

Probst

Scheid

2015

Current Opinion in Plant Biology

148

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Stress defense in plants is elaborated at the level of protection and adaptation. Dynamic changes in sophisticated chromatin substructures and concomitant transcriptional changes play an important role in response to stress, as illustrated by the transient rearrangement of compact heterochromatin structures or the modulation of chromatin composition and modification upon stress exposure. To connect cytological, developmental, and molecular data around stress and chromatin is currently an interesting, multifaceted, and sometimes controversial field of research. This review highlights some of the most recent findings on nuclear reorganization, histone variants, histone chaperones, DNA- and histone modifications, and somatic and meiotic heritability in connection with stress.

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Nuclear Architecture and Chromatin Dynamics in Interphase Nuclei of <b><i>Arabidopsis thaliana</i></b>

Cited by 21 publications

References 215 publications

Non-random chromosome arrangement in triploid endosperm nuclei

Non-random chromosome arrangement in triploid endosperm nuclei

Chromatin associations in Arabidopsis interphase nuclei

Stress-induced structural changes in plant chromatin

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